Differentiation-Associated Expression of Conventional Protein Kinase C Isoforms in Primary Cultures of Bone Marrow Cells Induced by M-CSF and G-CSF.

Differentiation-Associated Expression of Conventional Protein Kinase C Isoforms in Primary Cultures of Bone Marrow Cells Induced by M-CSF and G-CSF.

The Protein kinase C (PKC) -associated sign pathway performs essential roles in regulation of cell development, differentiation and apoptosis. The current research focuses on typical PKC (cPKC) expression and its regulation in major cultures of bone marrow cells induced to endure macrophage/granulocyte differentiation by macrophage colony-stimulating issue (M-CSF) or granular colony-stimulating issue (G-CSF).
By performing western blot evaluation with pan anti-PKC antibodies, we discovered that PKC is transiently induced by M-CSF, reaching a most degree by day 2, after which declines and diminishes by day 9 in major tradition of bone marrow cells. In distinction, the expression of PKC alongside G-CSF induced granulocytic differentiation of bone marrow stem cells is low and will increase regularly.
Reverse transcription-PCR (RT-PCR) assay was utilized to research the expression of PKC isoforms. PKCalpha is constitutively expressed in bone marrow cells independently of hematopoietic development components in cultures. PKC-gamma mRNA is undetectable. Equally, the expression of PKC-beta is transiently induced by M-CSF, but steadily elevated by G-CSF, in settlement with outcomes obtained from PKC protein expression.
Moreover, gel-shift assay confirmed that the activation of NF-kappaB is transiently induced by M-CSF however not by G-CSF. These information recommend that PKC expression is concerned in each macrophage and granulocyte differentiation by bone marrow dedicated stem cells. But, NF-kappaB activation is simply detected in macrophage and never granulocyte differentiation.
Thus, we conclude that the PKC-mediated signaling pathway is distinctly concerned in bone-marrow cell differentiation induced by M-CSF and G-CSF.

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