
Genetic variants of the human host influencing the coronavirus-associated phenotypes (SARS, MERS and COVID-19): rapid systematic review and field synopsis
The COVID 19th pandemic has strengthened interest in the biological mechanisms underlying the complex interactions between infectious agents and human hosts. The spectrum of phenotypes associated with SARS-CoV infection-2, from the absence of symptoms of severe systemic complications, raising questions about the extent to which the variable response to coronavirus (CoVs) is influenced by host genetic variability background.
To explore current knowledge about this question, we design a systematic review covers the scientific literature published from January 2003 to June 2020, to include the study of contemporary outbreaks caused by SARS-CoV-1, mer-CoV and SARS- CoV-2 (ie SARS, mer and COVID-19 disease). Studies were eligible if human genetic variants were tested as predictors of ad hoc phenotypes.An clinical protocol for a rapid review process is designed in accordance with the paradigm of PRISMA and registered in the database Prospero (ID: CRD42020180860).
Systematic workflow provided 32 articles eligible for data abstraction (28 on SARS, 1 in Mers, 3 in COVID-19) report data on 26 cohorts invention. Most studies considered a definite clinical diagnosis as the primary outcome, varies coupled with other results (severity is most often analyzed). Ten studies analyzed HLA haplotype (1 in patients with COVID-19) and did not provide a consistent signal relationship with phenotypes related diseases. Of the 22 articles that qualify investigating candidate genes (2 as associated with COVID-19), gen top ranking in a number of studies that ACE2, CLEC4M (L-SIGN), MBL, MxA (n = 3), ACE, CD209, FCER2 , OAS-1, TLR4, TNF-α (n = 2).
Only variants in the MBL and MxA found as a possible involvement in related CoV phenotype in at least two studies. The number of studies for each predictor is not enough to perform a meta-analyses.Studies collect large cohort from different ancestors needed to further clarify the role of genetic variants in determining the host response to infection CoVs. rigorous design and robust statistical method is guaranteed.

Classification discordant frequency variants in the Human Gene Mutation Database: Comparison of the American College of Medical Genetics and Genomics and ClinVar Guidelines
Purpose: variants of classification discordant between public databases is one of the well-documented limitations when interpreting the pathogenicity of variants. The purpose of this study was to determine the level of germline variants misannotation of Human Mutation Database Gen (HGMD) and concordance between database explanation.
Methods: We used a total of 188 106 classified variants (disease causing mutation [n = 179 454] and polymorphism [n = 8652]) in 6466 genes from HGMD. All variants were reanalyzed by the American College of Medical Genetics and Genomics (ACMG) guidelines and compared with the variant database ClinVar.
Recombinant Human Genome polyprotein, His-SUMO, E.coli-200ug |
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QP7370-ec-200ug | EnQuireBio | 200ug | EUR 634 |
Recombinant Human Genome polyprotein, His-SUMO, E.coli-500ug |
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QP7370-ec-500ug | EnQuireBio | 500ug | EUR 1060 |
Recombinant Human Genome polyprotein, His-SUMO, E.coli-50ug |
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QP7370-ec-50ug | EnQuireBio | 50ug | EUR 263 |
Recombinant Hepatitis C Virus polyprotein, His, E.coli-100ug |
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QP8939-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant Human Genome polyprotein, His, Yeast-10ug |
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QP7370-ye-10ug | EnQuireBio | 10ug | EUR 236 |
Recombinant Human Genome polyprotein, His, Yeast-1mg |
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QP7370-ye-1mg | EnQuireBio | 1mg | EUR 1885 |
Recombinant Human Genome polyprotein, His, Yeast-200ug |
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QP7370-ye-200ug | EnQuireBio | 200ug | EUR 744 |
Recombinant Human Genome polyprotein, His, Yeast-500ug |
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QP7370-ye-500ug | EnQuireBio | 500ug | EUR 1206 |
Recombinant Human Genome polyprotein, His, Yeast-50ug |
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QP7370-ye-50ug | EnQuireBio | 50ug | EUR 299 |
Genome polyprotein Antibody |
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1-CSB-PA333180LA01HFD | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Hepatitis C virus genotype 1a. This antibody is Unconjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody |
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1-CSB-PA714772LA01HYH | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is Unconjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody |
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1-CSB-PA18549A0Rb | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Dengue virus. This antibody is Unconjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody |
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1-CSB-PA362073LA01HQD | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human rhinovirus A serotype 89. This antibody is Unconjugated. Tested in the following application: ELISA |
Genome Polyprotein Antibody |
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20-abx109417 | Abbexa |
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Genome polyprotein Antibody |
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20-abx300967 | Abbexa |
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Recombinant Human TNNI2 Protein, His, E.coli-100ug |
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QP13776-HIS-100ug | EnQuireBio | 100ug | EUR 1261 |
Recombinant Human TNNI3 Protein, His, E.coli-100ug |
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QP13777-HIS-100ug | EnQuireBio | 100ug | EUR 218 |
Recombinant Human YWHAE Protein, His, E.coli-100ug |
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QP13985-HIS-100ug | EnQuireBio | 100ug | EUR 1261 |
Recombinant Human ACPP Protein, His, E.coli-100ug |
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QP10946-HIS-100ug | EnQuireBio | 100ug | EUR 1261 |
Recombinant Human ANGPTL3 Protein, His, E.coli-100ug |
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QP11018-HIS-100ug | EnQuireBio | 100ug | EUR 1261 |
Recombinant Human En Protein, His, E.coli-100ug |
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QP11784-HIS-100ug | EnQuireBio | 100ug | EUR 1261 |
Recombinant Human IGFBP7 Protein, His, E.coli-100ug |
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QP12384-HIS-100ug | EnQuireBio | 100ug | EUR 1261 |
Recombinant Human KLK5 Protein, His, E.coli-100ug |
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QP12507-HIS-100ug | EnQuireBio | 100ug | EUR 1261 |
Recombinant Human NARS Protein, His, E.coli-100ug |
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QP12794-HIS-100ug | EnQuireBio | 100ug | EUR 1261 |
Recombinant Human SLAMF1 Protein, His, E.coli-100ug |
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QP13518-HIS-100ug | EnQuireBio | 100ug | EUR 1261 |
Recombinant Human ALDH5A1, His-SUMO, E.coli-100ug |
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QP5645-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human COX4I1, His-SUMO, E.coli-100ug |
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QP5854-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human d2hgdh, His-SUMO, E.coli-100ug |
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QP7919-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human L2HGDH, His-SUMO, E.coli-100ug |
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QP8068-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human POLG2, His-SUMO, E.coli-100ug |
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QP8185-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human FDXR, His-SUMO, E.coli-100ug |
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QP6033-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human IDH3G, His-SUMO, E.coli-100ug |
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QP6194-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant E.coli ssbF Protein, His-SUMO, E.coli-100ug |
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QP7083-100ug | EnQuireBio | 100ug | EUR 771 |
Recombinant hepatitis Non-structural polyprotein pORF1 Protein, His, E.coli-100ug |
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QP7125-100ug | EnQuireBio | 100ug | EUR 771 |
Recombinant E.coli Agmatinase Protein, His-SUMO, E.coli-100ug |
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QP5622-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant E.coli nrdB Protein, His-SUMO, E.coli-100ug |
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QP6953-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant E.coli plsC Protein, His-SUMO, E.coli-100ug |
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QP7206-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant E.coli fkpA Protein, His-SUMO, E.coli-100ug |
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QP7261-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant E.coli nrdA Protein, His-SUMO, E.coli-100ug |
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QP7262-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant E.coli ruvA Protein, His-SUMO, E.coli-100ug |
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QP7321-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant E.coli fabF Protein, His-SUMO, E.coli-100ug |
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QP7326-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant E.coli LptA Protein, His-SUMO, E.coli-100ug |
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QP7331-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant E.coli Exopolyphosphatase Protein, His-SUMO, E.coli-100ug |
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QP7336-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant E.coli ruvB Protein, His-SUMO, E.coli-100ug |
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QP7392-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant E.coli ruvC Protein, His-SUMO, E.coli-100ug |
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QP7393-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant E.coli tetR Protein, His-SUMO, E.coli-100ug |
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QP7416-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Hepatitis C Genome polyprotein |
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1-CSB-RP184674Ba | Cusabio |
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Description: Recombinant Hepatitis C Genome polyprotein expressed in E.coli |
Genome Polyprotein Antibody (Biotin) |
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20-abx105887 | Abbexa |
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Genome polyprotein Antibody (Biotin) |
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20-abx106264 | Abbexa |
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Genome polyprotein Antibody (FITC) |
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20-abx107678 | Abbexa |
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Genome polyprotein Antibody (HRP) |
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20-abx109095 | Abbexa |
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Genome Polyprotein Polyclonal Antibody |
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A56130 | EpiGentek | 100 µg | EUR 570.55 |
Description: Ask the seller for details |
Genome Polyprotein Polyclonal Antibody |
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A56743 | EpiGentek | 100 µg | EUR 570.55 |
Description: Ask the seller for details |
Genome Polyprotein Polyclonal Antibody |
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A68253 | EpiGentek | 100 µg | EUR 570.55 |
Description: Ask the seller for details |
Genome Polyprotein Polyclonal Antibody |
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A68254 | EpiGentek | 100 µg | EUR 570.55 |
Description: reagents widely cited |
Genome polyprotein Antibody (HRP) |
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20-abx300968 | Abbexa |
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Genome polyprotein Antibody (FITC) |
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20-abx300969 | Abbexa |
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Genome polyprotein Antibody (Biotin) |
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20-abx300970 | Abbexa |
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Human rhinovirus 1A Genome polyprotein |
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1-CSB-YP326367HQA | Cusabio |
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Description: Recombinant Human rhinovirus 1A Genome polyprotein,partial expressed in Yeast |
Recombinant Zika Envelope Protein, His, E.coli-100ug |
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QP13997-HIS-100ug | EnQuireBio | 100ug | EUR 218 |
Recombinant Zika NS1 Protein, His, E.coli-100ug |
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QP14001-HIS-100ug | EnQuireBio | 100ug | EUR 218 |
Recombinant Mouse GSTP2 Protein, His, E.coli-100ug |
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QP12063-HIS-100ug | EnQuireBio | 100ug | EUR 1261 |
Recombinant Human CD274 Protein, His, E.coli-100ug |
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QP11319-HIS-EC-100ug | EnQuireBio | 100ug | EUR 1261 |
Recombinant Human AGRP Protein, His-SUMO, E.coli-100ug |
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QP5623-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human Adenosylhomocysteinase Protein, His-SUMO, E.coli-100ug |
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QP5629-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human AKR1C2 Protein, His-SUMO, E.coli-100ug |
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QP5637-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human AKT2 / Protein, His-SUMO, E.coli-100ug |
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QP5641-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human Asporin Protein, His-SUMO, E.coli-100ug |
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QP5687-ec-100ug | EnQuireBio | 100ug | EUR 571 |
Recombinant Human ATF2 Protein, His-SUMO, E.coli-100ug |
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QP5688-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human ATP5D Protein, His-SUMO, E.coli-100ug |
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QP5695-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human BCAT2 Protein, His-SUMO, E.coli-100ug |
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QP5707-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human BCL10 Protein, His-SUMO, E.coli-100ug |
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QP5709-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human BTN3A2 Protein, His-SUMO, E.coli-100ug |
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QP5730-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human BTN3A3 Protein, His-SUMO, E.coli-100ug |
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QP5731-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human CACNA2D1 Protein, His-SUMO, E.coli-100ug |
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QP5745-ec-100ug | EnQuireBio | 100ug | EUR 418 |
Recombinant Human CD7 Protein, His-SUMO, E.coli-100ug |
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QP5794-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human CDK5 Protein, His-SUMO, E.coli-100ug |
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QP5806-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human CEACAM6 Protein, His-SUMO, E.coli-100ug |
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QP5813-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human CEACAM7 Protein, His-SUMO, E.coli-100ug |
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QP5814-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human CIAO1 Protein, His-SUMO, E.coli-100ug |
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QP5836-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human CRIP2 Protein, His-SUMO, E.coli-100ug |
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QP5869-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human CYP11A1 Protein, His-SUMO, E.coli-100ug |
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QP5902-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human DCX Protein, His-SUMO, E.coli-100ug |
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QP5920-ec-100ug | EnQuireBio | 100ug | EUR 571 |
Recombinant Human Desmoplakin Protein, His-SUMO, E.coli-100ug |
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QP5952-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human EIF2AK2 Protein, His-SUMO, E.coli-100ug |
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QP5969-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human EIF3G Protein, His-SUMO, E.coli-100ug |
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QP5972-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human EIF4EBP3 Protein, His-SUMO, E.coli-100ug |
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QP5974-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human EML2 Protein, His-SUMO, E.coli-100ug |
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QP5982-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human BPHL Protein, His-SUMO, E.coli-100ug |
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QP7794-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human Vasculin Protein, His-SUMO, E.coli-100ug |
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QP7795-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human CHD1L Protein, His-SUMO, E.coli-100ug |
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QP7802-ec-100ug | EnQuireBio | 100ug | EUR 571 |
Recombinant Human VWA5B2 Protein, His-SUMO, E.coli-100ug |
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QP7823-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human ACBD4 Protein, His-SUMO, E.coli-100ug |
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QP7825-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human FANK1 Protein, His-SUMO, E.coli-100ug |
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QP7827-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Recombinant Human ABHD5 Protein, His-SUMO, E.coli-100ug |
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QP7831-ec-100ug | EnQuireBio | 100ug | EUR 571 |
Recombinant Human PTPMT1 Protein, His-SUMO, E.coli-100ug |
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QP7832-ec-100ug | EnQuireBio | 100ug | EUR 408 |
Results: When variants are classified by ACMG guidelines, misclassification was observed at 3.47% (2289 / 65,896) of variants. Overall suitability between HGMD and ClinVar is 97.62% (52,499 / 53,780) of the variants studied.
Conclusion: A variant in the database should be used with caution when variant pathogenicity interpreted. This study reveals misannotation frequency of variants and explanations HGMD compatibility between databases in depth.