Impacts of genomic networks governed by human-specific regulatory sequences and genetic loci harboring fixed human-specific neuro-regulatory single nucleotide mutations on phenotypic traits of modern humans

Impacts of genomic networks governed by human-specific regulatory sequences and genetic loci harboring fixed human-specific neuro-regulatory single nucleotide mutations on phenotypic traits of modern humans

recent advances in the identification and characterization of the human DNA sequence-specific regulation set the stage for a global assessment of their impact on the physiology and pathology of modern humans. Gene set enrichment analysis (GSEA) on 8405 35 074 human genes associated with neuro-regulatory-specific single nucleotide changes (hsSNCs) revealed numerous significant association with morphological structure, physiological processes and pathological conditions of modern man.

Significantly enhanced properties including more than 1000 different anatomical regions of the adult human brain, different types of cells and tissues, more than 200 human disorders are common, and more than 1000 records of rare diseases. HsSNCs thousands of genes connected with neuro-regulation have been identified, which represent an important genetic elements of autosomal inheritance and child survival phenotype. A total of 1,494 hsSNC-linked genes associated with either autosomal dominant or recessive, and 2273 genes related hsSNC has been linked to premature death, lethal embryos, as well as pre, peri, neo, and post-natal lethal phenotype of both complete and incomplete penetration.

Differential GSEA implemented on hsSNC-linked loci and genes associated to identify a set of 7990 hsSNC-target gene linked to a different class the evolution of human-specific regulatory sequence (hypersensitivity reaction). In particular, the expression of the majority of these genes (genes 5389; 67%) set by the sequence of stem cell-related retroviral (scar) and scar-regulated genes were arrested dominant fraction (91%) of the association of phenotype that was significantly associated with hsSNCs. MGI interrogation of databases revealed mouse models are available tailored to the exact experimental definition of the functional effects hsSNCs and scars on the causal genes that affect the phenotype of thousands of mammals and human beings are involved in hundreds of common and rare disorders.

These observations indicate that the dominant nature of certain men thrive under regulatory control combinations of different classes of a HSR and neuro-regulatory hsSNCs locus storing fixed in humans, is different from other primates, and is located in the region of chromatin vary accessed during brain development ,

 Impacts of genomic networks governed by human-specific regulatory sequences and genetic loci harboring fixed human-specific neuro-regulatory single nucleotide mutations on phenotypic traits of modern humans
Impacts of genomic networks governed by human-specific regulatory sequences and genetic loci harboring fixed human-specific neuro-regulatory single nucleotide mutations on phenotypic traits of modern humans

Genetic Diversity of Human Immunodeficiency Virus Type 1 in asymptomatic blood donors in Islamabad, Pakistan

The goal serological testing for human immunodeficiency virus (HIV) is mandatory under the security laws in the blood of Pakistan; Therefore, the existing data on the prevalence of HIV in the blood donor. However, little is known about the molecular epidemiology of HIV in the blood donor population.

Therefore, this study was designed to study the genetic diversity of HIV-1 infection in treatment-naïve population of apparently healthy blood donors in Islamabad, Pakistan. Materials and Methods A total of 85 736 blood donors tested for HIV by chemiluminescence immunoassay. All samples were positive donor were analyzed for the presence of various HIV genotypes (types and subtypes). viral ribonucleic acid extracted from blood samples from HIV-positive donors and reverse transcribed into a complementary deoxyribonucleic acid (cDNA).

Recombinant Human rhinovirus 1A Genome polyprotein, partial

CSB-YP326367HQA 6222 mg Ask for price

Recombinant Human rhinovirus 1A Genome polyprotein,partial

AP72911 each Ask for price

Recombinant Human rhinovirus 1A Genome polyprotein, partial

CSB-EP326367HQA 11613 mg Ask for price

Recombinant Human rhinovirus 1B Genome polyprotein, partial

CSB-EP320160HQB 10401 mg Ask for price

Recombinant Human rhinovirus 1A Genome polyprotein,partial

RPC25135-100ug 100ug
EUR 573.1

Recombinant Human rhinovirus 1A Genome polyprotein,partial

RPC25135-20ug 20ug
EUR 288.8

Rhinovirus A serotype 89 Genome polyprotein Antibody

20-abx109372
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Rhinovirus A serotype 89 Genome polyprotein Antibody

abx109372-100l 100 µl
EUR 162.5

Rhinovirus A serotype 89 Genome polyprotein Antibody (HRP)

20-abx108723
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  • 100 ug
  • 1 mg
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Rhinovirus A serotype 89 Genome polyprotein Antibody (HRP)

abx108723-100g 100 µg
EUR 362.5

Rhinovirus A serotype 89 Genome polyprotein Antibody (HRP)

abx108723-20g 20 µg
EUR 162.5

Rhinovirus A serotype 89 Genome polyprotein Antibody (HRP)

abx108723-50g 50 µg
EUR 250

Rhinovirus A serotype 89 Genome polyprotein Antibody (FITC)

20-abx107303
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  • 100 ug
  • 1 mg
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Rhinovirus A serotype 89 Genome polyprotein Antibody (FITC)

abx107303-100g 100 µg
EUR 362.5

Rhinovirus A serotype 89 Genome polyprotein Antibody (FITC)

abx107303-20g 20 µg
EUR 162.5

Rhinovirus A serotype 89 Genome polyprotein Antibody (FITC)

abx107303-50g 50 µg
EUR 250

Recombinant Human rhinovirus A serotype 89 Genome polyprotein, partial

CSB-YP362073HQD 6387 mg Ask for price

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial

AP71163 1mg
EUR 1978

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial

AP72984 each Ask for price

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial

AP77542 1mg
EUR 1978

Recombinant Human rhinovirus A serotype 89 Genome polyprotein, partial

CSB-EP362073HQD 3389 mg Ask for price

Recombinant Human rhinovirus A serotype 89 Genome polyprotein, partial

CSB-EP362073HQDb0 3390 mg Ask for price

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial

RPC22779-100ug 100ug
EUR 460.9

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial

RPC22779-1mg 1mg
EUR 2237.8

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial

RPC22779-20ug 20ug
EUR 258.7

Recombinant Human rhinovirus A serotype 89 Genome polyprotein, partial, Biotinylated

CSB-EP362073HQD-B 7058 mg Ask for price

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial,Biotinylated

RPC27677-100ug 100ug
EUR 614.3

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial,Biotinylated

RPC27677-1mg 1mg
EUR 2373.6

Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial,Biotinylated

RPC27677-20ug 20ug
EUR 468

Equine Rhinitis A Virus Genome Polyprotein Antibody

abx345840-100g 100 µg Ask for price

Equine Rhinitis A Virus Genome Polyprotein Antibody

abx345840-20g 20 µg
EUR 250

Equine Rhinitis A Virus Genome Polyprotein Antibody

abx345840-50g 50 µg
EUR 350

Genome Polyprotein Antibody

20-abx109417
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  • 100 ug
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Genome polyprotein Antibody

20-abx300967
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Genome polyprotein Antibody

1-CSB-PA18549A0Rb
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Dengue virus. This antibody is Unconjugated. Tested in the following application: ELISA

Genome polyprotein Antibody

1-CSB-PA333180LA01HFD
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  • 100ug
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Hepatitis C virus genotype 1a. This antibody is Unconjugated. Tested in the following application: ELISA

Genome polyprotein Antibody

1-CSB-PA362073LA01HQD
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  • 100ug
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human rhinovirus A serotype 89. This antibody is Unconjugated. Tested in the following application: ELISA

Genome polyprotein Antibody

CSB-PA4643XA11GSV 100ul / 50ul
EUR 299
Description: Genome polyprotein

Genome polyprotein Antibody

CSB-PA4644XA11GSW 100ul / 50ul
EUR 299
Description: Genome polyprotein

Genome polyprotein Antibody

1-CSB-PA714772LA01HYH
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is Unconjugated. Tested in the following application: ELISA

Genome Polyprotein Antibody

abx109417-100l 100 µl
EUR 162.5

Genome polyprotein Antibody

abx300967-100g 100 µg
EUR 362.5

Genome polyprotein Antibody

abx300967-20g 20 µg
EUR 162.5

Genome polyprotein Antibody

abx300967-50g 50 µg
EUR 250

Recombinant Human Genome polyprotein, His-SUMO, E.coli-10ug

QP7370-ec-10ug 10ug
EUR 240

Recombinant Human Genome polyprotein, His-SUMO, E.coli-50ug

QP7370-ec-50ug 50ug
EUR 315.6

Recombinant Human Genome polyprotein, His-SUMO, E.coli-1mg

QP7370-ec-1mg 1mg
EUR 1958.4

Recombinant Human Genome polyprotein, His-SUMO, E.coli-100ug

QP7370-ec-100ug 100ug
EUR 489.6

Recombinant Human Genome polyprotein, His-SUMO, E.coli-200ug

QP7370-ec-200ug 200ug
EUR 760.8

Recombinant Human Genome polyprotein, His-SUMO, E.coli-500ug

QP7370-ec-500ug 500ug
EUR 1272

Genome polyprotein Antibody (HRP)

20-abx109095
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  • 100 ug
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Genome polyprotein Antibody (HRP)

20-abx300968
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  • 100 ug
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Hepatitis C Genome polyprotein

1-CSB-RP184674Ba
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Description: Recombinant Hepatitis C Genome polyprotein expressed in E.coli

Genome polyprotein Antibody (HRP)

abx109095-100l 100 µl
EUR 162.5

Genome polyprotein Antibody (HRP)

abx300968-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (HRP)

abx300968-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (HRP)

abx300968-50g 50 µg
EUR 250

Genome polyprotein Antibody (FITC)

20-abx107678
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  • 100 ug
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Genome polyprotein Antibody (FITC)

20-abx300969
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  • 100 ug
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Genome polyprotein Antibody (FITC)

abx107678-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (FITC)

abx107678-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (FITC)

abx107678-50g 50 µg
EUR 250

Genome polyprotein Antibody (FITC)

abx300969-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (FITC)

abx300969-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (FITC)

abx300969-50g 50 µg
EUR 250

Genome Polyprotein Antibody (Biotin)

20-abx105887
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Genome polyprotein Antibody (Biotin)

20-abx106264
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  • 100 ug
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Genome polyprotein Antibody (Biotin)

20-abx300970
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  • 100 ug
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Genome Polyprotein Antibody (Biotin)

abx105887-100g 100 µg
EUR 362.5

Genome Polyprotein Antibody (Biotin)

abx105887-20g 20 µg
EUR 162.5

Genome Polyprotein Antibody (Biotin)

abx105887-50g 50 µg
EUR 250

Genome polyprotein Antibody (Biotin)

abx106264-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (Biotin)

abx106264-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (Biotin)

abx106264-50g 50 µg
EUR 250

Genome polyprotein Antibody (Biotin)

abx300970-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (Biotin)

abx300970-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (Biotin)

abx300970-50g 50 µg
EUR 250

Genome Polyprotein Polyclonal Antibody

A68253
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  • 100 µg
  • 20 ul
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  • 100 ul

Genome Polyprotein Polyclonal Antibody

A68254
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  • 100 µg
  • 20 ul
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  • 100 ul

Genome Polyprotein Polyclonal Antibody

A56130
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  • 100 µg
  • 20 ul
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  • 100 ul

Genome Polyprotein Polyclonal Antibody

A56743
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  • 100 µg
  • 20 ul
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  • 100 ul

Recombinant Human parechovirus 2 Genome polyprotein, partial

CSB-EP529147HXJ 9583 mg Ask for price

Recombinant Human parechovirus 1 Genome polyprotein

CSB-YP734409HCAV 9748 mg Ask for price

Yellow fever virus Genome polyprotein

1-CSB-EP365905YAC1
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  • 100ug
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  • 1MG
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  • 50ug
Description: Recombinant Yellow fever virus Genome polyprotein,partial expressed in E.coli

Genome Polyprotein (HCV-Core) Antibody

33487-05111 150 ug
EUR 215

Recombinant Human Genome polyprotein, His, Yeast-1mg

QP7370-ye-1mg 1mg
EUR 2262

Recombinant Human Genome polyprotein, His, Yeast-10ug

QP7370-ye-10ug 10ug
EUR 283.2

Recombinant Human Genome polyprotein, His, Yeast-50ug

QP7370-ye-50ug 50ug
EUR 358.8

Recombinant Human Genome polyprotein, His, Yeast-100ug

QP7370-ye-100ug 100ug
EUR 576

Recombinant Human Genome polyprotein, His, Yeast-200ug

QP7370-ye-200ug 200ug
EUR 892.8

Recombinant Human Genome polyprotein, His, Yeast-500ug

QP7370-ye-500ug 500ug
EUR 1447.2

Enterovirus 71 Genome polyprotein Antibody

20-abx109758
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
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Genome polyprotein Antibody, HRP conjugated

1-CSB-PA18549B0Rb
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Dengue virus. This antibody is HRP conjugated. Tested in the following application: ELISA

Genome polyprotein Antibody, HRP conjugated

1-CSB-PA333180LB01HFD
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Hepatitis C virus genotype 1a. This antibody is HRP conjugated. Tested in the following application: ELISA

Genome polyprotein Antibody, HRP conjugated

1-CSB-PA362073LB01HQD
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human rhinovirus A serotype 89. This antibody is HRP conjugated. Tested in the following application: ELISA

Genome polyprotein Antibody, HRP conjugated

1-CSB-PA714772LB01HYH
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is HRP conjugated. Tested in the following application: ELISA

Enterovirus 71 Genome polyprotein Antibody

abx109758-100l 100 µl
EUR 162.5

Recombinant Hepatitis C Genome polyprotein

CSB-RP184674Ba 5430 mg Ask for price

Genome polyprotein Antibody, FITC conjugated

1-CSB-PA18549C0Rb
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Dengue virus. This antibody is FITC conjugated. Tested in the following application: ELISA

Genome polyprotein Antibody, FITC conjugated

1-CSB-PA333180LC01HFD
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Hepatitis C virus genotype 1a. This antibody is FITC conjugated. Tested in the following application: ELISA

Genome polyprotein Antibody, FITC conjugated

1-CSB-PA362073LC01HQD
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human rhinovirus A serotype 89. This antibody is FITC conjugated. Tested in the following application: ELISA

Genome polyprotein Antibody, FITC conjugated

1-CSB-PA714772LC01HYH
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  • 100ug
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is FITC conjugated. Tested in the following application: ELISA

Recombinant Hepatitis C Genome polyprotein

RPC24458-100ug 100ug
EUR 721.6

Recombinant Hepatitis C Genome polyprotein

RPC24458-20ug 20ug
EUR 448.1

Genome polyprotein Antibody, Biotin conjugated

1-CSB-PA18549D0Rb
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Dengue virus. This antibody is Biotin conjugated. Tested in the following application: ELISA

Genome polyprotein Antibody, Biotin conjugated

1-CSB-PA333180LD01HFD
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Hepatitis C virus genotype 1a. This antibody is Biotin conjugated. Tested in the following application: ELISA

Genome polyprotein Antibody, Biotin conjugated

1-CSB-PA362073LD01HQD
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human rhinovirus A serotype 89. This antibody is Biotin conjugated. Tested in the following application: ELISA

Genome polyprotein Antibody, Biotin conjugated

1-CSB-PA714772LD01HYH
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is Biotin conjugated. Tested in the following application: ELISA

Japanese encephalitis virus Genome polyprotein

1-CSB-EP189574Ba
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  • 100ug
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  • 1MG
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  • 50ug
Description: Recombinant Japanese encephalitis virus Genome polyprotein,partial expressed in E.coli

Recombinant Human parechovirus 1 Genome polyprotein, partial

CSB-EP734409HCAV 9651 mg Ask for price

Enterovirus 71 Genome polyprotein Antibody (HRP)

20-abx108220
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Enterovirus 71 Genome polyprotein Antibody (HRP)

abx108220-100g 100 µg
EUR 362.5

Enterovirus 71 Genome polyprotein Antibody (HRP)

abx108220-20g 20 µg
EUR 162.5

Enterovirus 71 Genome polyprotein Antibody (HRP)

abx108220-50g 50 µg
EUR 250

Recombinant JEV Genome polyprotein (aa 2528-3432) Protein

VAng-Wyb3447-1mgEcoli 1 mg (E. coli)
EUR 9926.4
Description: Japanese encephalitis virus (strain Jaoars982) Genome polyprotein, recombinant protein.

Enterovirus 71 Genome polyprotein Antibody (FITC)

20-abx106801
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
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Enterovirus 71 Genome polyprotein Antibody (FITC)

abx106801-100g 100 µg
EUR 362.5

Enterovirus 71 Genome polyprotein Antibody (FITC)

abx106801-20g 20 µg
EUR 162.5

Enterovirus 71 Genome polyprotein Antibody (FITC)

abx106801-50g 50 µg
EUR 250

Recombinant Human parechovirus 2 Genome polyprotein, partial, Biotinylated

CSB-EP897051HHAL-B 10843 mg Ask for price

Anti-CrPV Genome polyprotein Polyclonal Antibody

AB1I144 0.1mg
EUR 399

Enterovirus 71 Genome polyprotein Antibody (Biotin)

20-abx105382
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  • 100 ug
  • 1 mg
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Recombinant Zika virus Genome polyprotein, partial

CSB-EP3643GOZ3 7769 mg Ask for price

Enterovirus 71 Genome polyprotein Antibody (Biotin)

abx105382-100g 100 µg
EUR 362.5

Enterovirus 71 Genome polyprotein Antibody (Biotin)

abx105382-20g 20 µg
EUR 162.5

Enterovirus 71 Genome polyprotein Antibody (Biotin)

abx105382-50g 50 µg
EUR 250

Hepatitis C virus genotype 1b Genome polyprotein

1-CSB-YP530838HVQ(A3)
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  • 100ug
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  • 1MG
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Description: Recombinant Hepatitis C virus genotype 1b Genome polyprotein,partial expressed in Yeast

Hepatitis C virus genotype 1b Genome polyprotein

1-CSB-YP530838HVQ(A4)
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  • 100ug
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  • 1MG
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Description: Recombinant Hepatitis C virus genotype 1b Genome polyprotein,partial expressed in Yeast

Hepatitis C virus genotype 1a Genome polyprotein

1-CSB-EP333180HFD
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  • 100ug
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  • 1MG
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  • 50ug
Description: Recombinant Hepatitis C virus genotype 1a Genome polyprotein,partial expressed in E.coli

Hepatitis C virus genotype 1a Genome polyprotein

1-CSB-MP333180HFD
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  • 100ug
  • 1MG
  • 200ug
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Description: Recombinant Hepatitis C virus genotype 1a Genome polyprotein,partial expressed in Mammalian cell

Porcine Teschovirus 1 Genome Polyprotein Antibody

abx345841-100g 100 µg Ask for price

Porcine Teschovirus 1 Genome Polyprotein Antibody

abx345841-20g 20 µg
EUR 250

Porcine Teschovirus 1 Genome Polyprotein Antibody

abx345841-50g 50 µg
EUR 350

Genome Polyprotein Polyclonal Antibody, HRP Conjugated

A68252
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  • 100 µg
  • 50 ul
  • 100 ul

Genome Polyprotein Polyclonal Antibody, HRP Conjugated

A68255
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  • 100 µg
  • 50 ul
  • 100 ul

Genome Polyprotein Polyclonal Antibody, HRP Conjugated

A56131
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  • 100 µg
  • 50 ul
  • 100 ul

Genome Polyprotein Polyclonal Antibody, HRP Conjugated

A56744
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  • 100 µg
  • 50 ul
  • 100 ul

Recombinant Dengue virus type 2 Genome polyprotein, partial

CSB-YP530838DCF 7267 mg Ask for price

Genome Polyprotein Polyclonal Antibody, FITC Conjugated

A68251
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  • 100 µg
  • 50 ul
  • 100 ul

Genome Polyprotein Polyclonal Antibody, FITC Conjugated

A68256
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  • 100 µg
  • 50 ul
  • 100 ul

Genome Polyprotein Polyclonal Antibody, FITC Conjugated

A56132
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  • 100 µg
  • 50 ul
  • 100 ul

Genome Polyprotein Polyclonal Antibody, FITC Conjugated

A56745
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  • 100 µg
  • 50 ul
  • 100 ul

Recombinant Enterovirus A71 Genome polyprotein, partial

CSB-EP3114GLA 7385 mg Ask for price

Recombinant Enterovirus A71 Genome polyprotein, partial

CSB-EP3114GLAa2 7384 mg Ask for price

Recombinant Enterovirus A71 Genome polyprotein, partial

CSB-EP3114GLAe0 7511 mg Ask for price

Recombinant Enterovirus A71 Genome polyprotein, partial

CSB-EP3114GLAe1 7917 mg Ask for price

Recombinant Enterovirus A71 Genome polyprotein,partial

RPC28548-100ug 100ug
EUR 801.9

Recombinant Enterovirus A71 Genome polyprotein,partial

RPC28548-1mg 1mg
EUR 2885.2

Recombinant Enterovirus A71 Genome polyprotein,partial

RPC28548-20ug 20ug
EUR 448.1

Recombinant Enterovirus A71 Genome polyprotein,partial

RPC28549-100ug 100ug
EUR 801.9

Recombinant Enterovirus A71 Genome polyprotein,partial

RPC28549-1mg 1mg
EUR 2885.2

Recombinant Enterovirus A71 Genome polyprotein,partial

RPC28549-20ug 20ug
EUR 448.1

The cDNA of all positive donor were then analyzed for the presence of various HIV genotypes (types and subtypes) using the primer specific subtype in a nested polymerase chain reaction. Products powered run on ethidium bromide-stained 2% agarose gel and visualized using ultraviolet Transilluminator. A particular subtype is assigned to a specific subtype samples if the reaction made the band a very intense 20% compared to the band made by subtype-independent reaction.

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