Title- Genomic landscape of squamous cell carcinoma- Different genetic pathways culminating in a common phenotype

Title- Genomic landscape of squamous cell carcinoma- Different genetic pathways culminating in a common phenotype

Introduction: Squamous cell carcinomas (SqCCs) are the commonest stable tumors in people and are discovered throughout a number of organ programs. Though, built-in evaluation of genetic alterations expose similarities between SqCCs from varied physique websites, sure genes seem like extra regularly mutated in a given SqCC. These delicate variations could maintain the important thing to figuring out the differentiation traits and predicting aggressiveness of tumors.
Supplies and technique: Fifty-four instances of SqCCs, during which the first location of the tumor may very well be ascertained by medical and radiological findings, have been included on this research. Subsequent era sequencing knowledge was analyzed for recurrent genetic abnormalities.
Outcomes: Genetic alterations have been present in 219 genes within the 54 instances studied. TP53 mutations have been discovered to be extra frequent in pulmonary SqCCs (86.5%) as in comparison with non-pulmonary SqCCs (58.8%) (p<0.05). NOTCH gene household mutations and CREBBP mutations have been restricted to non-pulmonary SqCC (p<0.005) and have been mutated in 41.2% and 17.6% instances.
Conclusion: An in depth comparative evaluation of the genetic alterations recognized by sequencing recognized larger frequency of TP53 mutations in lung SqCCs as in comparison with non-pulmonary SqCCs. NOTCH and CREBPP mutations have been discovered to be absent in lung and head and neck SqCCs and extra frequent in SqCCs from different areas

The genetic foundation for the evolution of soma: mechanistic proof for the co-option of a stress-induced gene right into a developmental grasp regulator

In multicellular organisms with specialised cells, essentially the most important distinction amongst cell sorts is between reproductive (germ) cells and non-reproductive/somatic cells (soma). Though soma contributed to the marked enhance in complexity of many multicellular lineages, little is understood about its evolutionary origins. We’ve beforehand instructed that the evolution of genes answerable for the differentiation of somatic cells concerned the co-option of life historical past trade-off genes that in unicellular organisms enhanced survival at a price to speedy copy. Within the multicellular inexperienced alga, Volvox carteri, cell destiny is established early in growth by the differential expression of a grasp regulatory gene often known as regA.
A carefully associated RegA-Like Sequence (RLS1) is current in its single-celled relative, Chlamydomonas reinhardtiiRLS1 is expressed in response to emphasize, and we proposed that an environmentally induced RLS1-like gene was co-opted right into a developmental pathway within the lineage resulting in V. carteri. Nonetheless, the precise evolutionary situation answerable for the postulated co-option occasion stays to be decided. Right here, we present that along with being developmentally regulated, regA will also be induced by environmental cues, indicating that regA has maintained its ancestral regulation.
We additionally discovered that the absence of a practical RegA protein confers elevated sensitivity to emphasize, in keeping with RegA having a direct or oblique function in stress responses. Total, this research (i) supplies mechanistic proof for the co-option of an environmentally induced gene into a serious developmental regulator, (ii) helps the view that main morphological improvements can evolve through regulatory modifications and (iii) argues for the function of stress within the evolution of multicellular complexity.
 Title- Genomic landscape of squamous cell carcinoma- Different genetic pathways culminating in a common phenotype
Title- Genomic landscape of squamous cell carcinoma- Different genetic pathways culminating in a common phenotype

Chromosome-level de novo meeting of Coprinopsis cinerea A43mut B43mut pab1-1 #326 and genetic variant identification of mutants utilizing Nanopore MinION sequencing

The homokaryotic Coprinopsis cinerea pressure A43mut B43mut pab1-1 #326 is a extensively used experimental mannequin for developmental research in mushroom-forming fungi. It may possibly develop on outlined synthetic media and full the entire lifecycle inside two weeks. The mutations in mating sort components A and B end result within the particular function of clamp formation and fruiting with out mating. This function permits investigations and manipulations with a homokaryotic genetic background. Present genome meeting of pressure #326 was based mostly on short-read sequencing knowledge and was extremely fragmented, resulting in the bias in gene annotation and downstream analyses.
Right here, we report a chromosome-level genome meeting of pressure #326. Oxford Nanopore Expertise (ONT) MinION sequencing was used to get lengthy reads. Illumina brief reads was used to shine the sequences. A mixed meeting yield 13 chromosomes and a mitochondrial genome as particular person scaffolds. The meeting has 15,250 annotated genes with a excessive synteny with the C. cinerea pressure Okayama-7 #130. This meeting has nice enchancment on contiguity and annotations. It’s a appropriate reference for additional genomic research, particularly for the genetic, genomic and transcriptomic analyses in ONT lengthy reads. Single nucleotide variants and structural variants in six mutagenized and cisplatin-screened mutants may very well be recognized and validated. A 66 bp deletion in Ras GTPase-activating protein (RasGAP) was present in all mutants.

Recombinant Hepatitis C virus genotype 1a Genome polyprotein, partial

CSB-MP333180HFD 4998 mg Ask for price

Recombinant Hepatitis C virus genotype 1b Genome polyprotein,partial

AP73727 each Ask for price

Recombinant Hepatitis C virus genotype 1b Genome polyprotein,partial

AP73728 each Ask for price

Hepatitis C Genome polyprotein

1-CSB-RP184674Ba
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Description: Recombinant Hepatitis C Genome polyprotein expressed in E.coli

Recombinant Hepatitis C virus genotype 1a (isolate 1) (HCV) Genome polyprotein(E1),partial

AP70969 1mg
EUR 2826

Recombinant Hepatitis C Genome polyprotein

CSB-RP184674Ba 5430 mg Ask for price

Recombinant Hepatitis C Genome polyprotein

RPC24458-100ug 100ug
EUR 721.6

Recombinant Hepatitis C Genome polyprotein

RPC24458-20ug 20ug
EUR 448.1

Hepatitis E virus genotype 1 Non-structural polyprotein pORF1 (ORF1)

1-CSB-EP327230HGE
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Description: Recombinant Hepatitis E virus genotype 1 Non-structural polyprotein pORF1(ORF1),partial expressed in E.coli

Recombinant Hepatitis E virus genotype 1 Non-structural polyprotein pORF1 (ORF1)

AP71039 1mg
EUR 2826

Recombinant Hepatitis C Virus polyprotein, His, E.coli-1mg

QP8939-ec-1mg 1mg
EUR 2763.6

Equine Rhinitis A Virus Genome Polyprotein Antibody

abx345840-100g 100 µg Ask for price

Equine Rhinitis A Virus Genome Polyprotein Antibody

abx345840-20g 20 µg
EUR 250

Equine Rhinitis A Virus Genome Polyprotein Antibody

abx345840-50g 50 µg
EUR 350

Yellow fever virus Genome polyprotein

1-CSB-EP365905YAC1
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Description: Recombinant Yellow fever virus Genome polyprotein,partial expressed in E.coli

Recombinant Hepatitis C Virus polyprotein, His, E.coli-10ug

QP8939-ec-10ug 10ug
EUR 391.2

Recombinant Hepatitis C Virus polyprotein, His, E.coli-50ug

QP8939-ec-50ug 50ug
EUR 522

Recombinant Hepatitis C Virus polyprotein, His, E.coli-100ug

QP8939-ec-100ug 100ug
EUR 848.4

Recombinant Hepatitis C Virus polyprotein, His, E.coli-200ug

QP8939-ec-200ug 200ug
EUR 1338

Recombinant Hepatitis C Virus polyprotein, His, E.coli-500ug

QP8939-ec-500ug 500ug
EUR 1816.8

Recombinant Hepatitis C virus genotype 1a (isolate 1) polyprotein, His-SUMO, Mammal-1mg

QP6996-1mg 1mg
EUR 3037.2

Recombinant Hepatitis E virus genotype 1 Non-structural polyprotein pORF1 (ORF1), partial

CSB-EP327230HGE 2937 mg Ask for price

Recombinant Hepatitis C virus genotype 1b (strain HC-J4) polyprotein, His, Yeast-1mg

QP9739-ye-1mg 1mg
EUR 3296.4

Recombinant Hepatitis C virus genotype 1a (isolate 1) polyprotein, His-SUMO, Mammal-10ug

QP6996-10ug 10ug
EUR 426

Recombinant Hepatitis C virus genotype 1a (isolate 1) polyprotein, His-SUMO, Mammal-200ug

QP6996-200ug 200ug
EUR 1468.8

Recombinant Hepatitis C virus genotype 1a (isolate 1) polyprotein, His-SUMO, Mammal-20ug

QP6996-20ug 20ug
EUR 523.2

Recombinant Hepatitis C virus genotype 1a (isolate 1) polyprotein, His-SUMO, Mammal-500ug

QP6996-500ug 500ug
EUR 1936.8

Recombinant Hepatitis C virus genotype 1b (strain HC-J4) polyprotein, His, Yeast-100ug

QP9739-ye-100ug 100ug
EUR 945.6

Recombinant Hepatitis C virus genotype 1b (strain HC-J4) polyprotein, His, Yeast-10ug

QP9739-ye-10ug 10ug
EUR 434.4

Recombinant Hepatitis C virus genotype 1b (strain HC-J4) polyprotein, His, Yeast-200ug

QP9739-ye-200ug 200ug
EUR 1512

Recombinant Hepatitis C virus genotype 1b (strain HC-J4) polyprotein, His, Yeast-500ug

QP9739-ye-500ug 500ug
EUR 2164.8

Recombinant Hepatitis C virus genotype 1b (strain HC-J4) polyprotein, His, Yeast-50ug

QP9739-ye-50ug 50ug
EUR 576

Japanese encephalitis virus Genome polyprotein

1-CSB-EP189574Ba
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Description: Recombinant Japanese encephalitis virus Genome polyprotein,partial expressed in E.coli

Recombinant Zika virus Genome polyprotein, partial

CSB-EP3643GOZ3 7769 mg Ask for price

Japanese Encephalitis Virus Genome polyprotein Antibody

abx345000-100l 100 µl
EUR 350

Japanese Encephalitis Virus Genome polyprotein Antibody

abx345000-50l 50 µl
EUR 250

Bovine Viral Diarrhea Virus Genome Polyprotein Antibody

abx345377-100tests 100 tests
EUR 250

Recombinant West Nile virus Genome polyprotein, partial

CSB-EP356974WAF2 9323 mg Ask for price

Recombinant Yellow fever virus Genome polyprotein, partial

CSB-EP365905YAC1 3485 mg Ask for price

Recombinant Yellow fever virus Genome polyprotein,partial

AP77947 1mg
EUR 2826

Recombinant Yellow fever virus Genome polyprotein,partial

RPC22821-100ug 100ug
EUR 721.6

Recombinant Yellow fever virus Genome polyprotein,partial

RPC22821-1mg 1mg
EUR 2884.7

Recombinant Yellow fever virus Genome polyprotein,partial

RPC22821-20ug 20ug
EUR 448.1

Recombinant Japanese encephalitis virus Genome polyprotein, partial

CSB-EP189574Ba 2537 mg Ask for price

Recombinant Bovine viral diarrhea virus Genome polyprotein , partial

CSB-YP312464BKX1 6140 mg Ask for price

Recombinant Bovine viral diarrhea virus Genome polyprotein, partial

CSB-EP312464BKX1 2704 mg Ask for price

Recombinant Bovine viral diarrhea virus Genome polyprotein, partial

CSB-BP312464BKX1 139 mg Ask for price

Recombinant Bovine viral diarrhea virus Genome polyprotein,partial

RPC28130-100ug 100ug
EUR 1544.5

Recombinant Bovine viral diarrhea virus Genome polyprotein,partial

RPC28130-20ug 20ug
EUR 580.8

Recombinant Dengue virus type 2 Genome polyprotein, partial

CSB-YP530838DCF 7267 mg Ask for price

Recombinant Classical swine fever virus Genome polyprotein, partial

CSB-YP4953GLU 12085 mg Ask for price

Recombinant St. louis encephalitis virus Genome polyprotein, partial

CSB-EP357746SKT 9322 mg Ask for price

Recombinant Classical swine fever virus Genome polyprotein, partial

CSB-EP4953GLU 11663 mg Ask for price

Recombinant Classical swine fever virus Genome polyprotein, partial

CSB-BP4334GLU 10139 mg Ask for price

Classical Swine Fever Virus Genome Polyprotein (CSFV-GP) Protein

abx694331-10nmol 10 nmol
EUR 625

Classical Swine Fever Virus Genome Polyprotein (CSFV-GP) Protein

abx694331-5nmol 5 nmol
EUR 425

Recombinant Tick-borne encephalitis virus Genome polyprotein, partial

CSB-EP3056GKR 2646 mg Ask for price

Recombinant Dengue virus type 2 Genome polyprotein (Q1642N), partial

CSB-EP3044GLD(M) 11736 mg Ask for price

Genome polyprotein Antibody

20-abx300967
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Genome polyprotein Antibody

1-CSB-PA18549A0Rb
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  • 100ug
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Dengue virus. This antibody is Unconjugated. Tested in the following application: ELISA

Genome polyprotein Antibody

1-CSB-PA333180LA01HFD
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Hepatitis C virus genotype 1a. This antibody is Unconjugated. Tested in the following application: ELISA

Genome polyprotein Antibody

1-CSB-PA362073LA01HQD
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human rhinovirus A serotype 89. This antibody is Unconjugated. Tested in the following application: ELISA

Genome polyprotein Antibody

CSB-PA4643XA11GSV 100ul / 50ul
EUR 299
Description: Genome polyprotein

Genome polyprotein Antibody

CSB-PA4644XA11GSW 100ul / 50ul
EUR 299
Description: Genome polyprotein

Genome polyprotein Antibody

1-CSB-PA714772LA01HYH
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is Unconjugated. Tested in the following application: ELISA

Genome Polyprotein Antibody

20-abx109417
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Genome polyprotein Antibody

abx300967-100g 100 µg
EUR 362.5

Genome polyprotein Antibody

abx300967-20g 20 µg
EUR 162.5

Genome polyprotein Antibody

abx300967-50g 50 µg
EUR 250

Genome Polyprotein Antibody

abx109417-100l 100 µl
EUR 162.5

Recombinant Dengue virus 2 Genome polyprotein (T2493G,G2494S,I2762T), partial

CSB-EP2239GLD(M) 11735 mg Ask for price

Genome polyprotein Antibody (HRP)

20-abx300968
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  • 100 ug
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Genome polyprotein Antibody (HRP)

20-abx109095
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  • 100 ug
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Genome polyprotein Antibody (HRP)

abx300968-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (HRP)

abx300968-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (HRP)

abx300968-50g 50 µg
EUR 250

Genome polyprotein Antibody (HRP)

abx109095-100l 100 µl
EUR 162.5

Genome polyprotein Antibody (FITC)

20-abx300969
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  • 100 ug
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Genome polyprotein Antibody (FITC)

20-abx107678
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  • 100 ug
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Genome polyprotein Antibody (FITC)

abx300969-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (FITC)

abx300969-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (FITC)

abx300969-50g 50 µg
EUR 250

Genome polyprotein Antibody (FITC)

abx107678-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (FITC)

abx107678-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (FITC)

abx107678-50g 50 µg
EUR 250

Genome polyprotein Antibody (Biotin)

20-abx300970
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  • 100 ug
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Genome Polyprotein Antibody (Biotin)

20-abx105887
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  • 100 ug
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Genome polyprotein Antibody (Biotin)

20-abx106264
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  • 100 ug
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Genome polyprotein Antibody (Biotin)

abx300970-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (Biotin)

abx300970-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (Biotin)

abx300970-50g 50 µg
EUR 250

Genome Polyprotein Antibody (Biotin)

abx105887-100g 100 µg
EUR 362.5

Genome Polyprotein Antibody (Biotin)

abx105887-20g 20 µg
EUR 162.5

Genome Polyprotein Antibody (Biotin)

abx105887-50g 50 µg
EUR 250

Genome polyprotein Antibody (Biotin)

abx106264-100g 100 µg
EUR 362.5

Genome polyprotein Antibody (Biotin)

abx106264-20g 20 µg
EUR 162.5

Genome polyprotein Antibody (Biotin)

abx106264-50g 50 µg
EUR 250

Genome Polyprotein Polyclonal Antibody

A56130
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  • 100 µg
  • 20 ul
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  • 100 ul

Genome Polyprotein Polyclonal Antibody

A56743
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  • 100 µg
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  • 100 ul

Genome Polyprotein Polyclonal Antibody

A68253
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  • 100 µg
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  • 100 ul

Genome Polyprotein Polyclonal Antibody

A68254
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  • 100 µg
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  • 100 ul

Recombinant Tick-borne encephalitis virus European subtype Genome polyprotein, partial

CSB-EP318509TEM 11863 mg Ask for price

Anti-CrPV Genome polyprotein Polyclonal Antibody

AB1I144 0.1mg
EUR 399

Genome Polyprotein (HCV-Core) Antibody

33487-05111 150 ug
EUR 215

Rhinovirus A serotype 89 Genome polyprotein Antibody

20-abx109372
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
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Rhinovirus A serotype 89 Genome polyprotein Antibody

abx109372-100l 100 µl
EUR 162.5

Recombinant Tick-borne encephalitis virus Far Eastern subtype Genome polyprotein, partial

CSB-EP357187TEN 11862 mg Ask for price

Recombinant Tick-borne encephalitis virus Far Eastern subtype Genome polyprotein, partial

CSB-EP357187TENa0 11843 mg Ask for price

Recombinant Tick-borne encephalitis virus Far Eastern subtype Genome polyprotein, partial

CSB-EP357187TENc7 11839 mg Ask for price

Human rhinovirus A serotype 89 Genome polyprotein

1-CSB-YP362073HQD
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Description: Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial expressed in Yeast

Human rhinovirus A serotype 89 Genome polyprotein

1-CSB-EP362073HQD
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  • 100ug
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  • 1MG
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Description: Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial expressed in E.coli

Human rhinovirus A serotype 89 Genome polyprotein

1-CSB-EP362073HQDb0
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Description: Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial expressed in E.coli

Genome polyprotein Antibody, HRP conjugated

1-CSB-PA18549B0Rb
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Dengue virus. This antibody is HRP conjugated. Tested in the following application: ELISA

Genome polyprotein Antibody, HRP conjugated

1-CSB-PA333180LB01HFD
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Hepatitis C virus genotype 1a. This antibody is HRP conjugated. Tested in the following application: ELISA

Genome polyprotein Antibody, HRP conjugated

1-CSB-PA362073LB01HQD
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human rhinovirus A serotype 89. This antibody is HRP conjugated. Tested in the following application: ELISA

Genome polyprotein Antibody, HRP conjugated

1-CSB-PA714772LB01HYH
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is HRP conjugated. Tested in the following application: ELISA

Enterovirus 71 Genome polyprotein Antibody

20-abx109758
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  • 100 ug
  • 1 mg
  • 200 ug
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Enterovirus 71 Genome polyprotein Antibody

abx109758-100l 100 µl
EUR 162.5

Porcine Teschovirus 1 Genome Polyprotein Antibody

abx345841-100g 100 µg Ask for price

Porcine Teschovirus 1 Genome Polyprotein Antibody

abx345841-20g 20 µg
EUR 250

Porcine Teschovirus 1 Genome Polyprotein Antibody

abx345841-50g 50 µg
EUR 350

Genome polyprotein Antibody, FITC conjugated

1-CSB-PA18549C0Rb
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Dengue virus. This antibody is FITC conjugated. Tested in the following application: ELISA
To make a greater use of ONT sequencing platform, we modified a high-molecular-weight genomic DNA isolation protocol based mostly on magnetic beads for filamentous fungi. This research confirmed using MinION to assemble a fungal reference genome and to carry out downstream research in a person laboratory. An experimental workflow was proposed, from DNA isolation and complete genome sequencing, to genome meeting and variant calling. Our outcomes offered options and parameters for fungal genomic evaluation on MinION sequencing platform.

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