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Title- Genomic landscape of squamous cell carcinoma- Different genetic pathways culminating in a common phenotype
Introduction: Squamous cell carcinomas (SqCCs) are the commonest stable tumors in people and are discovered throughout a number of organ programs. Though, built-in evaluation of genetic alterations expose similarities between SqCCs from varied physique websites, sure genes seem like extra regularly mutated in a given SqCC. These delicate variations could maintain the important thing to figuring out the differentiation traits and predicting aggressiveness of tumors.
Supplies and technique: Fifty-four instances of SqCCs, during which the first location of the tumor may very well be ascertained by medical and radiological findings, have been included on this research. Subsequent era sequencing knowledge was analyzed for recurrent genetic abnormalities.
Outcomes: Genetic alterations have been present in 219 genes within the 54 instances studied. TP53 mutations have been discovered to be extra frequent in pulmonary SqCCs (86.5%) as in comparison with non-pulmonary SqCCs (58.8%) (p<0.05). NOTCH gene household mutations and CREBBP mutations have been restricted to non-pulmonary SqCC (p<0.005) and have been mutated in 41.2% and 17.6% instances.
Conclusion: An in depth comparative evaluation of the genetic alterations recognized by sequencing recognized larger frequency of TP53 mutations in lung SqCCs as in comparison with non-pulmonary SqCCs. NOTCH and CREBPP mutations have been discovered to be absent in lung and head and neck SqCCs and extra frequent in SqCCs from different areas
The genetic foundation for the evolution of soma: mechanistic proof for the co-option of a stress-induced gene right into a developmental grasp regulator
In multicellular organisms with specialised cells, essentially the most important distinction amongst cell sorts is between reproductive (germ) cells and non-reproductive/somatic cells (soma). Though soma contributed to the marked enhance in complexity of many multicellular lineages, little is understood about its evolutionary origins. We’ve beforehand instructed that the evolution of genes answerable for the differentiation of somatic cells concerned the co-option of life historical past trade-off genes that in unicellular organisms enhanced survival at a price to speedy copy. Within the multicellular inexperienced alga, Volvox carteri, cell destiny is established early in growth by the differential expression of a grasp regulatory gene often known as regA.
A carefully associated RegA-Like Sequence (RLS1) is current in its single-celled relative, Chlamydomonas reinhardtii. RLS1 is expressed in response to emphasize, and we proposed that an environmentally induced RLS1-like gene was co-opted right into a developmental pathway within the lineage resulting in V. carteri. Nonetheless, the precise evolutionary situation answerable for the postulated co-option occasion stays to be decided. Right here, we present that along with being developmentally regulated, regA will also be induced by environmental cues, indicating that regA has maintained its ancestral regulation.
We additionally discovered that the absence of a practical RegA protein confers elevated sensitivity to emphasize, in keeping with RegA having a direct or oblique function in stress responses. Total, this research (i) supplies mechanistic proof for the co-option of an environmentally induced gene into a serious developmental regulator, (ii) helps the view that main morphological improvements can evolve through regulatory modifications and (iii) argues for the function of stress within the evolution of multicellular complexity.

Chromosome-level de novo meeting of Coprinopsis cinerea A43mut B43mut pab1-1 #326 and genetic variant identification of mutants utilizing Nanopore MinION sequencing
The homokaryotic Coprinopsis cinerea pressure A43mut B43mut pab1-1 #326 is a extensively used experimental mannequin for developmental research in mushroom-forming fungi. It may possibly develop on outlined synthetic media and full the entire lifecycle inside two weeks. The mutations in mating sort components A and B end result within the particular function of clamp formation and fruiting with out mating. This function permits investigations and manipulations with a homokaryotic genetic background. Present genome meeting of pressure #326 was based mostly on short-read sequencing knowledge and was extremely fragmented, resulting in the bias in gene annotation and downstream analyses.
Right here, we report a chromosome-level genome meeting of pressure #326. Oxford Nanopore Expertise (ONT) MinION sequencing was used to get lengthy reads. Illumina brief reads was used to shine the sequences. A mixed meeting yield 13 chromosomes and a mitochondrial genome as particular person scaffolds. The meeting has 15,250 annotated genes with a excessive synteny with the C. cinerea pressure Okayama-7 #130. This meeting has nice enchancment on contiguity and annotations. It’s a appropriate reference for additional genomic research, particularly for the genetic, genomic and transcriptomic analyses in ONT lengthy reads. Single nucleotide variants and structural variants in six mutagenized and cisplatin-screened mutants may very well be recognized and validated. A 66 bp deletion in Ras GTPase-activating protein (RasGAP) was present in all mutants.
Genome polyprotein Antibody |
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1-CSB-PA333180LA01HFD | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Hepatitis C virus genotype 1a. This antibody is Unconjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody |
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1-CSB-PA362073LA01HQD | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human rhinovirus A serotype 89. This antibody is Unconjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody |
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1-CSB-PA714772LA01HYH | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is Unconjugated. Tested in the following application: ELISA |
Goat Anti-Hepatitis A Virus (Strain HM175) IgG |
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HAV11-A | Alpha Diagnostics | 100 ul | EUR 548.4 |
Human rhinovirus A serotype 89 Genome polyprotein |
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1-CSB-YP362073HQD | Cusabio |
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Description: Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial expressed in Yeast |
Human rhinovirus A serotype 89 Genome polyprotein |
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1-CSB-EP362073HQD | Cusabio |
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Description: Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial expressed in E.coli |
Human rhinovirus A serotype 89 Genome polyprotein |
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1-CSB-EP362073HQDb0 | Cusabio |
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Description: Recombinant Human rhinovirus A serotype 89 Genome polyprotein,partial expressed in E.coli |
Genome Polyprotein Antibody |
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20-abx109417 | Abbexa |
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Genome polyprotein Antibody |
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20-abx300967 | Abbexa |
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Duck Hepatitis Virus (DHV) ELISA Kit |
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abx054976-96tests | Abbexa | 96 tests | EUR 801.6 |
Rhinovirus A serotype 89 Genome polyprotein Antibody |
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20-abx109372 | Abbexa |
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Genome polyprotein Antibody, HRP conjugated |
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1-CSB-PA18549B0Rb | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Dengue virus. This antibody is HRP conjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody, FITC conjugated |
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1-CSB-PA18549C0Rb | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Dengue virus. This antibody is FITC conjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody, Biotin conjugated |
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1-CSB-PA18549D0Rb | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Dengue virus. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody, HRP conjugated |
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1-CSB-PA333180LB01HFD | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Hepatitis C virus genotype 1a. This antibody is HRP conjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody, FITC conjugated |
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1-CSB-PA333180LC01HFD | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Hepatitis C virus genotype 1a. This antibody is FITC conjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody, Biotin conjugated |
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1-CSB-PA333180LD01HFD | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Hepatitis C virus genotype 1a. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody, HRP conjugated |
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1-CSB-PA362073LB01HQD | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human rhinovirus A serotype 89. This antibody is HRP conjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody, FITC conjugated |
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1-CSB-PA362073LC01HQD | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human rhinovirus A serotype 89. This antibody is FITC conjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody, Biotin conjugated |
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1-CSB-PA362073LD01HQD | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human rhinovirus A serotype 89. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Human rhinovirus 1A Genome polyprotein |
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1-CSB-YP326367HQA | Cusabio |
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Description: Recombinant Human rhinovirus 1A Genome polyprotein,partial expressed in Yeast |
Genome polyprotein Antibody, HRP conjugated |
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1-CSB-PA714772LB01HYH | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is HRP conjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody, FITC conjugated |
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1-CSB-PA714772LC01HYH | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is FITC conjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody, Biotin conjugated |
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1-CSB-PA714772LD01HYH | Cusabio |
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Description: A polyclonal antibody against Genome polyprotein. Recognizes Genome polyprotein from Human enterovirus 71. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Genome polyprotein Antibody (HRP) |
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20-abx109095 | Abbexa |
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Genome Polyprotein Antibody (Biotin) |
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20-abx105887 | Abbexa |
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Genome polyprotein Antibody (Biotin) |
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20-abx106264 | Abbexa |
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Genome polyprotein Antibody (FITC) |
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20-abx107678 | Abbexa |
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Genome polyprotein Antibody (HRP) |
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20-abx300968 | Abbexa |
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Genome polyprotein Antibody (FITC) |
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20-abx300969 | Abbexa |
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Genome polyprotein Antibody (Biotin) |
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20-abx300970 | Abbexa |
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Genome Polyprotein Polyclonal Antibody |
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A68253-020 | EpiGentek | 20 ul | EUR 117.7 |
Genome Polyprotein Polyclonal Antibody |
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A68253-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody |
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A68253-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody |
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A68254-020 | EpiGentek | 20 ul | EUR 117.7 |
Genome Polyprotein Polyclonal Antibody |
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A68254-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody |
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A68254-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody |
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A56130-020 | EpiGentek | 20 ul | EUR 117.7 |
Genome Polyprotein Polyclonal Antibody |
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A56130-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody |
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A56130-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody |
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A56743-020 | EpiGentek | 20 ul | EUR 117.7 |
Genome Polyprotein Polyclonal Antibody |
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A56743-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody |
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A56743-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody |
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A56130 | EpiGentek |
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Genome Polyprotein Polyclonal Antibody |
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A56743 | EpiGentek |
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Genome Polyprotein Polyclonal Antibody |
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A68253 | EpiGentek |
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Genome Polyprotein Polyclonal Antibody |
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A68254 | EpiGentek |
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Hepatitis E virus genotype 1 Non-structural polyprotein pORF1 (ORF1) |
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1-CSB-EP327230HGE | Cusabio |
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Description: Recombinant Hepatitis E virus genotype 1 Non-structural polyprotein pORF1(ORF1),partial expressed in E.coli |
Rhinovirus A serotype 89 Genome polyprotein Antibody (HRP) |
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20-abx108723 | Abbexa |
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Rhinovirus A serotype 89 Genome polyprotein Antibody (FITC) |
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20-abx107303 | Abbexa |
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Rabbit Anti-Hemagglutinin HA1 Influenza A Virus (H11N2; A/duck/Yangzhou/906/2002) IgG |
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H11N2-01-A | Alpha Diagnostics | 100 ul | EUR 578.4 |
Hepatitis A Virus protein |
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30R-AH015 | Fitzgerald | 1 ml | EUR 453.6 |
Description: Purified native Hepatitis A Virus protein |
Hepatitis A Virus protein |
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30-1929 | Fitzgerald | 1 ml | EUR 159.6 |
Description: Purified Native Hepatitis A Virus protein |
Hepatitis A Virus antibody |
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10-2693 | Fitzgerald | 1 mg | EUR 198 |
Description: Mouse Monoclonal Hepatitis A Virus antibody |
Hepatitis A Virus antibody |
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10-H15A | Fitzgerald | 100 ug | EUR 462 |
Description: Mouse monoclonal Hepatitis A Virus antibody |
Hepatitis A Virus antibody |
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10-H15B | Fitzgerald | 100 ug | EUR 462 |
Description: Mouse monoclonal Hepatitis A Virus antibody |
Hepatitis A Virus Antibody |
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abx021766-1mg | Abbexa | 1 mg | EUR 2114.4 |
Hepatitis A Virus Antibody |
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abx021767-1mg | Abbexa | 1 mg | EUR 2890.8 |
Genome Polyprotein (HCV-Core) Antibody |
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33487-05111 | AssayPro | 150 ug | EUR 313.2 |
Enterovirus 71 Genome polyprotein Antibody |
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20-abx109758 | Abbexa |
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Recombinant Hepatitis C Virus polyprotein, His, E.coli-100ug |
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QP8939-ec-100ug | EnQuireBio | 100ug | EUR 848.4 |
Recombinant Hepatitis C Virus polyprotein, His, E.coli-10ug |
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QP8939-ec-10ug | EnQuireBio | 10ug | EUR 391.2 |
Recombinant Hepatitis C Virus polyprotein, His, E.coli-1mg |
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QP8939-ec-1mg | EnQuireBio | 1mg | EUR 2763.6 |
Recombinant Hepatitis C Virus polyprotein, His, E.coli-200ug |
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QP8939-ec-200ug | EnQuireBio | 200ug | EUR 1338 |
Recombinant Hepatitis C Virus polyprotein, His, E.coli-500ug |
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QP8939-ec-500ug | EnQuireBio | 500ug | EUR 1816.8 |
Recombinant Hepatitis C Virus polyprotein, His, E.coli-50ug |
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QP8939-ec-50ug | EnQuireBio | 50ug | EUR 522 |
Virus Hepatitis A Virus (HAV) Protein |
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abx670259-1ml | Abbexa | 1 ml | EUR 427.2 |
Duck Hepatitis Virus Antibody (DHV-Ab) ELISA Kit |
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abx054977-96tests | Abbexa | 96 tests | EUR 801.6 |
Mouse Anti-Hepatitis A Virus (VP3) IgG |
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HAV12-M | Alpha Diagnostics | 100 ul | EUR 578.4 |
Sindbis virus Structural polyprotein |
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1-CSB-CF361018SHZ | Cusabio |
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Description: Recombinant Sindbis virus Structural polyprotein,partial expressed in in vitro E.coli expression system |
Sindbis virus Structural polyprotein |
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1-CSB-CF361018SHZb2 | Cusabio |
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Description: Recombinant Sindbis virus Structural polyprotein,partial expressed in in vitro E.coli expression system |
Turnip mosaic virus Polyprotein |
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1-CSB-EP2708TJM | Cusabio |
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Description: Recombinant Turnip mosaic virus Polyprotein,partial expressed in E.coli |
Goat Anti-Hepatitis A virus (anti-HAV) IgG |
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HAV11-S | Alpha Diagnostics | 100 ul | EUR 343.2 |
Recombinant Hepatitis A Virus VP1 |
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7-07384 | CHI Scientific | 100µg | Ask for price |
Recombinant Hepatitis A Virus VP1 |
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7-07385 | CHI Scientific | 500µg | Ask for price |
Recombinant Hepatitis A Virus VP1 |
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7-07386 | CHI Scientific | 1000µg | Ask for price |
Recombinant Hepatitis A Virus P2C |
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7-07393 | CHI Scientific | 100µg | Ask for price |
Recombinant Hepatitis A Virus P2C |
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7-07394 | CHI Scientific | 500µg | Ask for price |
Recombinant Hepatitis A Virus P2C |
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7-07395 | CHI Scientific | 1000µg | Ask for price |
Recombinant Hepatitis A Virus P3C |
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7-07402 | CHI Scientific | 100µg | Ask for price |
Recombinant Hepatitis A Virus P3C |
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7-07403 | CHI Scientific | 500µg | Ask for price |
Recombinant Hepatitis A Virus P3C |
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7-07404 | CHI Scientific | 1000µg | Ask for price |
Recombinant Hepatitis A Virus VP3 |
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7-07405 | CHI Scientific | 100µg | Ask for price |
Recombinant Hepatitis A Virus VP3 |
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7-07406 | CHI Scientific | 500µg | Ask for price |
Recombinant Hepatitis A Virus VP3 |
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7-07407 | CHI Scientific | 1000µg | Ask for price |
Hepatitis A Virus (HAV) Antibody |
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abx024089-1mg | Abbexa | 1 mg | EUR 1412.4 |
Hepatitis A Virus (HAV) Antibody |
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abx024090-1mg | Abbexa | 1 mg | EUR 1412.4 |
Hepatitis A Virus (HAV) Antibody |
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abx024091-1mg | Abbexa | 1 mg | EUR 1412.4 |
Hepatitis A Virus VP3 Protein |
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20-abx260118 | Abbexa |
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Hepatitis A Virus VP1 Protein |
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20-abx260119 | Abbexa |
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Hepatitis A Virus P2C Protein |
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20-abx260121 | Abbexa |
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Hepatitis A Virus P3C Protein |
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20-abx260122 | Abbexa |
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Hepatitis A Virus (HAV) Antigens |
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HAV17-N-10 | Alpha Diagnostics | 10 ug | Ask for price |
Hepatitis A virus PCR kit |
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PCR-H495-48R | Bioingentech | 50T | EUR 987.6 |
Hepatitis A virus PCR kit |
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PCR-H495-96R | Bioingentech | 100T | EUR 1335.6 |
HAVCR1 Human, Hepatitis A Virus Cellular Receptor 1 Human Recombinant Protein, HEK |
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PROTQ96D42-1 | BosterBio | Regular: 10ug | EUR 380.4 |
Description: HAVCR1 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (Ser21-Thr288) containing a total of 283 amino acids, having a calculated molecular mass of 30.5kDa. HAVCR1 is fused to a 2 aa N-terminal linker, a 2 aa C-terminal linker and a 6 aa His tag at C-Terminus. |
Enterovirus 71 Genome polyprotein Antibody (HRP) |
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20-abx108220 | Abbexa |
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Enterovirus 71 Genome polyprotein Antibody (Biotin) |
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20-abx105382 | Abbexa |
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Enterovirus 71 Genome polyprotein Antibody (FITC) |
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20-abx106801 | Abbexa |
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Genome Polyprotein Polyclonal Antibody, Biotin Conjugated |
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A68250-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody, Biotin Conjugated |
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A68250-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody, FITC Conjugated |
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A68251-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody, FITC Conjugated |
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A68251-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody, HRP Conjugated |
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A68252-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody, HRP Conjugated |
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A68252-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody, HRP Conjugated |
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A68255-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody, HRP Conjugated |
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A68255-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody, FITC Conjugated |
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A68256-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody, FITC Conjugated |
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A68256-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody, Biotin Conjugated |
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A68257-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody, Biotin Conjugated |
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A68257-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody, HRP Conjugated |
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A56131-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody, HRP Conjugated |
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A56131-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody, FITC Conjugated |
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A56132-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody, FITC Conjugated |
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A56132-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody, Biotin Conjugated |
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A56133-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody, Biotin Conjugated |
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A56133-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody, HRP Conjugated |
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A56744-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody, HRP Conjugated |
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A56744-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody, FITC Conjugated |
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A56745-050 | EpiGentek | 50 ul | EUR 302.5 |
Genome Polyprotein Polyclonal Antibody, FITC Conjugated |
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A56745-100 | EpiGentek | 100 ul | EUR 423.5 |
Genome Polyprotein Polyclonal Antibody, Biotin Conjugated |
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A56746-050 | EpiGentek | 50 ul | EUR 302.5 |
×
To make a greater use of ONT sequencing platform, we modified a high-molecular-weight genomic DNA isolation protocol based mostly on magnetic beads for filamentous fungi. This research confirmed using MinION to assemble a fungal reference genome and to carry out downstream research in a person laboratory. An experimental workflow was proposed, from DNA isolation and complete genome sequencing, to genome meeting and variant calling. Our outcomes offered options and parameters for fungal genomic evaluation on MinION sequencing platform.
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