fragment of Rv3194c positioned on mycobacterial cell flooring successfully prevents adhesion of recombinant Mycobacterium smegmatis, and ensures a model new
September 25, 2020
Microscale Perfusion-based Cultivation for Pichia pastoris Clone Screening Enables Accelerated and Optimized RecombinantProtein Production Processes
Pichia pastoris has emerged before now years as a promising host for recombinant protein and biopharmaceutical manufacturing. Inside the establishment of extreme cell density fed-batch biomanufacturing, screening half and early bioprocess progress (based totally on microplates and shake flasks) nonetheless characterize a bottleneck attributable to high-cost and time-consuming procedures along with low experiment complexity.
Inside the present work, we implement a screening protocol developed for P. pastoris clone alternative in a multiplexed microfluidic machine with 15 μL cultivation chambers ready to operate in perfusion mode and monitor dissolved oxygen content material materials throughout the custom in a non-invasive means.
The setup allowed us to establish carbon-limited conditions and think about stress responses to completely completely different enter variables. Outcomes from micro-scale perfusion cultures had been then in distinction with 1L fed-batch fermentation.
The simplest producer by the use of titer and productiveness was shortly acknowledged after 12 hours from inoculation and the outcomes confirmed by lab-scale fermentation.
Moreover, the physiological analyses of the strains beneath completely completely different conditions steered how further sophisticated experimental conditions had been achievable whatever the comparatively easy, straight-forward, and cost-effective experimental setup.
Implementation and standardization of these micro-scale protocols might in the reduction of the demand for lab-scale bioreactor cultivations thus accelerating the occasion of protein manufacturing processes. This textual content is protected by copyright. All rights reserved.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EN1 (Engrailed 1) (N-term). This antibody is tested and proven to work in the following applications:
Chicken Homeobox Protein Engrailed-2 (EN2) ELISA Kit
Description: A Monoclonal antibody against Human EN1 / Engrailed (clone 1F5). The antibodies are raised in Mouse and are from clone 1F5. This antibody is applicable in WB and IHC-P, E
Description: A Monoclonal antibody against Human EN1 / Engrailed (clone 3H2). The antibodies are raised in Mouse and are from clone 3H2. This antibody is applicable in WB and IHC-P, E
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Guinea pig Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Guinea pig Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Guinea pig Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Homeobox protein engrailed-2 in samples from serum, plasma, tissue homogenates and other biological fluids.
Should the Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates or other biological fluids.
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit
Should the Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates or other biological fluids.
Description: Quantitativesandwich ELISA kit for measuring Human Homeobox protein engrailed-2 (EN2) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Homeobox protein engrailed-2(EN2) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Human Engrailed Homeobox Protein 2 ELISA Kit (EN2)
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Engrailed Homeobox Protein 2 (EN2) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Engrailed Homeobox Protein 2 (EN2) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Engrailed Homeobox Protein 2 (EN2) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Engrailed Homeobox Protein 2 (EN2) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids.
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit
Known also as Engrailed Homeobox Protein 2 elisa. Alternative names of the recognized antigen: AUTS1
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: CD40 Ligand (CD40LG) is a transmembrane protein that is a member of the TNF superfamily. In addition to existing in a membrane bound form, CD40LG can also be released as a soluble molecule (sCD40LG), which has been shown to have biological functions.CD40 interacts with CD40 ligand (aka CD40LG, TNFSF5 or CD154), which is predominantly expressed on activated CD4+ T-cells. Chicken CD40 Ligand Recombinant Protein is purified CD40LG produced in yeast.
ELISA kit for Human EN2 (Engrailed Homeobox Protein 2)
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Engrailed Homeobox Protein 2 (EN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to
Description: A sandwich ELISA kit for detection of Engrailed Homeobox Protein 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: IL-10 is an anti-inflammatory cytokine and a member of the IL-10 family of cytokines, which have indispensable functions in many infectious and inflammatory diseases. Chicken IL-10 Recombinant Protein is purified interleukin-10 produced in yeast.
Description: Interleukin-18 (IL-18) is a proinflammatory cytokine that belongs to the IL-1 superfamily. IL-18 works together with IL-12 to induce cell-mediated immunity following infection, and IL-18 is also able to induce severe inflammatory reactions, which suggests its role in certain inflammatory disorders. Chicken IL-18 Recombinant Protein is purified interleukin-18 produced in yeast.
Description: IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. Chicken IL-4 Recombinant Protein is purified interleukin-4 produced in yeast.
Description: Interleukin 16 (IL-16) is a pleiotropic cytokine that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4, and it has effects on mixed lymphocyte reaction and inhibition of HIV viral replication. Chicken IL-16 Recombinant Protein is purified interleukin-16 produced in yeast.
Description: IL-17F induces the production of many other cytokines, chemokines, and prostaglandins from many cell types (fibroblasts, endothelial cells, epithelial cells, keratinocytes and macrophages). IL-17F has been shown to have a pro-inflammatory role in asthma. Chicken IL-17F Recombinant Protein is purified interleukin-17F produced in yeast.
Description: The IL-12 p40 subunit, or interleukin-12 beta subunit, is the 40 kD cytokine receptor-like subunit of the heterodimeric cytokines IL-12 and IL-23. IL-12 is involved in the differentiation of naive T cells into Th1 cells and the growth and function of T cells. Both IL-23 and IL-12 can activate the transcription activator STAT4 and stimulate the production of interferon-gamma (IFNG). Chicken IL-12 p40 Recombinant Protein is purified interleukin-12 p40 produced in yeast.
Description: POU5F1 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 360 amino acids and having a molecular mass of 38.6kDa.;The POU5F1 is fused to a C-terminal poly-arginine tag and purified by proprietary chromatographic techniques.
A protein fragment of Rv3194c positioned on mycobacterial cell flooring successfully prevents adhesion of recombinant Mycobacterium smegmatis, and ensures a model new anti-adhesive drug
Adhesins are virulence components expressed on the surfaces of pathogenic micro organism that mediate pathogen-host interactions, a vital step throughout the an an infection course of.
Proper right here, we current that the Mycobacterium tuberculosis protease Rv3194c capabilities not solely as an enzyme nevertheless as an adhesin. The heterologous Rv3194c protein was purified from Escherichia coli and was confirmed to bind to hyaluronic acid (HA).
The HA-binding website was acknowledged as a 20 amino acid peptide between residues 91 and 110 (P91-110). Rv3194c certain to A549 alveolar basal epithelial cells and the interaction was abolished by the addition of hyaluronidase or P91-110.
Experimental an an infection in vitro revealed that Rv3194c participates throughout the attachment of recombinant Mycobacterium smegmatis (Rv3194c/MS) to A549 cells, and P91-110 treatment of A549 cells largely inhibited the Rv3194c/MS-A549 cell interaction.
To supply in vivo proof, we constructed a reporter stress of M. smegmatis that expressed a by-product of the firefly luciferase that is shifted to pink (FFlucRT) along with Rv3194c (Rv3194c + FFlucRT/MS) to infect mice and monitor the event of the sickness.
In mice, Rv3194c dramatically enhanced M. smegmatis persistence and induced lesions throughout the lungs. In addition to, treatment of intratracheal Rv3194c + FFlucRT/MS- contaminated mice with P91-110 significantly suppressed the growth of Rv3194c + FFlucRT/MS in vivo and diminished pathological injury introduced on by an an infection of the lung with Rv3194c + FFlucRT/MS.
Taken collectively, these outcomes reveal that Rv3194c capabilities as an HA-binding adhesin and that P91-110 may need the potential for treating and stopping mycobacterial an an infection.
Lysine acylation using conjugating enzymes for site-specific modification and ubiquitination of recombinantproteins
Enzymes are extremely efficient devices for protein labelling attributable to their specificity and delicate response conditions. Many protocols, nonetheless, are restricted to modifications at protein termini, depend upon non-peptidic metabolites or require large recognition domains. Proper right here we report a chemoenzymatic methodology, which we title lysine acylation using conjugating enzymes (LACE), to site-specifically modify folded proteins at inside lysine residues. LACE depends upon a minimal genetically encoded tag (Four residues) acknowledged by the E2 small ubiquitin-like modifier-conjugating enzyme Ubc9, and peptide or protein thioesters.
Collectively, this methodology obviates the need for E1 and E3 enzymes, enabling isopeptide formation with merely Ubc9 in a programmable methodology. We reveal the utility of LACE by the site-specific attachment of biochemical probes, one-pot dual-labelling collectively with sortase, and the conjugation of wild-type ubiquitin and ISG15 to recombinant proteins.
Should the Bovine Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Alpha-Fetoprotein (aFP) in samples from serum, plasma or other biological fluids.
Should the Bovine Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Alpha-Fetoprotein (aFP) in samples from serum, plasma or other biological fluids.
Should the Canine Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Alpha-Fetoprotein (aFP) in samples from serum, plasma or other biological fluids.
Should the Canine Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Alpha-Fetoprotein (aFP) in samples from serum, plasma or other biological fluids.
Should the Equine Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Equine Alpha-Fetoprotein (aFP) in samples from serum, plasma, tissue homogenates or other biological fluids.
Should the Equine Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Equine Alpha-Fetoprotein (aFP) in samples from serum, plasma, tissue homogenates or other biological fluids.
Should the Human Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Alpha-Fetoprotein (aFP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Human Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Alpha-Fetoprotein (aFP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Mouse Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Alpha-Fetoprotein (aFP) in samples from serum, plasma, tissue homogenates or other biological fluids.
Should the Mouse Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Alpha-Fetoprotein (aFP) in samples from serum, plasma, tissue homogenates or other biological fluids.
Should the Porcine Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Alpha-Fetoprotein (aFP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Porcine Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Alpha-Fetoprotein (aFP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Rat Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Alpha-Fetoprotein (aFP) in samples from serum, plasma, tissue homogenates or other biological fluids.
Should the Rat Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Alpha-Fetoprotein (aFP) in samples from serum, plasma, tissue homogenates or other biological fluids.
Should the Monkey Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Monkey Alpha-Fetoprotein (aFP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Monkey Alpha-Fetoprotein (aFP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Monkey Alpha-Fetoprotein (aFP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: The substance Filipin III is a polyene macrolide antibiotic and antifungal. It is from streptomyces filipinensis and has a purity of >98%. The pure substance is white or slightly yellow powder which is May be dissolved in methanol (10 mg/mL), ethanol (2 mg/mL) or DMSO (3 mg/mL).
Filipin III (Polyene macrolide antibiotic and antifungal)
Description: The substance Filipin III is a polyene macrolide antibiotic and antifungal. It is from streptomyces filipinensis and has a purity of >98%. The pure substance is white or slightly yellow powder which is May be dissolved in methanol (10 mg/mL), ethanol (2 mg/mL) or DMSO (3 mg/mL).
Description: AFP produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 597 amino acids (19-609a.a.) and having a molecular mass of 67.3kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa)._x000D_ _x000D_ AFP is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.