fragment of Rv3194c positioned on mycobacterial cell flooring successfully prevents adhesion of recombinant Mycobacterium smegmatis, and ensures a model new
Microscale Perfusion-based Cultivation for Pichia pastoris Clone Screening Enables Accelerated and Optimized RecombinantProtein Production Processes
Pichia pastoris has emerged before now years as a promising host for recombinant protein and biopharmaceutical manufacturing. Inside the establishment of extreme cell density fed-batch biomanufacturing, screening half and early bioprocess progress (based totally on microplates and shake flasks) nonetheless characterize a bottleneck attributable to high-cost and time-consuming procedures along with low experiment complexity.
Inside the present work, we implement a screening protocol developed for P. pastoris clone alternative in a multiplexed microfluidic machine with 15 μL cultivation chambers ready to operate in perfusion mode and monitor dissolved oxygen content material materials throughout the custom in a non-invasive means.
The setup allowed us to establish carbon-limited conditions and think about stress responses to completely completely different enter variables. Outcomes from micro-scale perfusion cultures had been then in distinction with 1L fed-batch fermentation.
The simplest producer by the use of titer and productiveness was shortly acknowledged after 12 hours from inoculation and the outcomes confirmed by lab-scale fermentation.
Moreover, the physiological analyses of the strains beneath completely completely different conditions steered how further sophisticated experimental conditions had been achievable whatever the comparatively easy, straight-forward, and cost-effective experimental setup.
Implementation and standardization of these micro-scale protocols might in the reduction of the demand for lab-scale bioreactor cultivations thus accelerating the occasion of protein manufacturing processes. This textual content is protected by copyright. All rights reserved.
Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-10ug |
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QP5983-ec-10ug | EnQuireBio | 10ug | EUR 391.2 |
Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-200ug |
|||
QP5983-ec-200ug | EnQuireBio | 200ug | EUR 1338 |
Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-500ug |
|||
QP5983-ec-500ug | EnQuireBio | 500ug | EUR 1816.8 |
Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-50ug |
|||
QP5983-ec-50ug | EnQuireBio | 50ug | EUR 522 |
Chicken Homeobox protein engrailed- 2, EN2 ELISA KIT |
|||
ELI-05953c | Lifescience Market | 96 Tests | EUR 1113.6 |
Chicken Homeobox Protein Engrailed-2 (EN2) ELISA Kit |
|||
abx518615-96tests | Abbexa | 96 tests | EUR 1093.2 |
Chicken EN2 / Homeobox protein engrailed-2 ELISA Kit |
|||
E1851c | EIAab | 96T | EUR 900 |
Recombinant Engrailed Homeobox Protein 2 (EN2) |
|||
RPU55393-100ug | Biomatik Corporation | 100ug | EUR 468.6 |
Recombinant Engrailed Homeobox Protein 2 (EN2) |
|||
RPU55393-1mg | Biomatik Corporation | 1mg | EUR 2074.8 |
Recombinant Engrailed Homeobox Protein 2 (EN2) |
|||
RPU55393-50ug | Biomatik Corporation | 50ug | EUR 376.2 |
Recombinant Engrailed Homeobox Protein 2 (EN2) |
|||
4-RPJ982Hu01 | Cloud-Clone |
|
|
Description: Recombinant Human Engrailed Homeobox Protein 2 expressed in: E.coli |
Homeobox Protein Engrailed-1 (EN1) Antibody |
|||
abx033297-400ul | Abbexa | 400 ul | EUR 627.6 |
Homeobox Protein Engrailed-1 (EN1) Antibody |
|||
abx033297-80l | Abbexa | 80 µl | EUR 343.2 |
Homeobox Protein Engrailed-1 (EN1) Antibody |
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20-abx215150 | Abbexa |
|
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Homeobox Protein Engrailed-1 (EN1) Antibody |
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20-abx300471 | Abbexa |
|
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Homeobox Protein Engrailed-1 (EN1) Antibody |
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abx033297-100g | Abbexa | 100 µg | EUR 281.25 |
Homeobox Protein Engrailed-1 (EN1) Antibody |
|||
abx300471-100g | Abbexa | 100 µg | EUR 362.5 |
Homeobox Protein Engrailed-1 (EN1) Antibody |
|||
abx300471-20g | Abbexa | 20 µg | EUR 162.5 |
Homeobox Protein Engrailed-1 (EN1) Antibody |
|||
abx300471-50g | Abbexa | 50 µg | EUR 250 |
Chicken Engrailed Homeobox Protein 2 ELISA kit |
|||
E01A91538 | BlueGene | 96T | EUR 700 |
Description: ELISA |
Homeobox Protein Engrailed-1 (EN1) Antibody (HRP) |
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20-abx300472 | Abbexa |
|
|
Homeobox Protein Engrailed-1 (EN1) Antibody (HRP) |
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abx300472-100g | Abbexa | 100 µg | EUR 362.5 |
Homeobox Protein Engrailed-1 (EN1) Antibody (HRP) |
|||
abx300472-20g | Abbexa | 20 µg | EUR 162.5 |
Homeobox Protein Engrailed-1 (EN1) Antibody (HRP) |
|||
abx300472-50g | Abbexa | 50 µg | EUR 250 |
Homeobox Protein Engrailed-1 (EN1) Antibody (FITC) |
|||
20-abx300473 | Abbexa |
|
|
Homeobox Protein Engrailed-1 (EN1) Antibody (FITC) |
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abx300473-100g | Abbexa | 100 µg | EUR 362.5 |
Homeobox Protein Engrailed-1 (EN1) Antibody (FITC) |
|||
abx300473-20g | Abbexa | 20 µg | EUR 162.5 |
Homeobox Protein Engrailed-1 (EN1) Antibody (FITC) |
|||
abx300473-50g | Abbexa | 50 µg | EUR 250 |
Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin) |
|||
20-abx300474 | Abbexa |
|
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Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin) |
|||
abx300474-100g | Abbexa | 100 µg | EUR 362.5 |
Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin) |
|||
abx300474-20g | Abbexa | 20 µg | EUR 162.5 |
Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin) |
|||
abx300474-50g | Abbexa | 50 µg | EUR 250 |
Mouse Homeobox protein engrailed- 1, En1 ELISA KIT |
|||
ELI-27118m | Lifescience Market | 96 Tests | EUR 1038 |
Human Homeobox protein engrailed- 1, EN1 ELISA KIT |
|||
ELI-48646h | Lifescience Market | 96 Tests | EUR 988.8 |
Human Homeobox protein engrailed-1 (EN1) ELISA Kit |
|||
abx527211-96tests | Abbexa | 96 tests | EUR 687.5 |
Mouse Homeobox protein engrailed-1 (EN1) ELISA Kit |
|||
abx527212-96tests | Abbexa | 96 tests | EUR 687.5 |
EN1 (untagged)-Human engrailed homeobox 1 (EN1) |
|||
SC303016 | Origene Technologies GmbH | 10 µg | Ask for price |
EN1 (GFP-tagged) - Human engrailed homeobox 1 (EN1) |
|||
RG224659 | Origene Technologies GmbH | 10 µg | Ask for price |
EN1 (Myc-DDK-tagged)-Human engrailed homeobox 1 (EN1) |
|||
RC224659 | Origene Technologies GmbH | 10 µg | Ask for price |
Homeobox protein engrailed-2 |
|||
AP79211 | SAB | 1mg | EUR 2640 |
Homeobox protein engrailed-2 |
|||
AP83697 | SAB | 1mg | EUR 2640 |
Homeobox protein engrailed-2 |
|||
AP84048 | SAB | 1mg | EUR 2640 |
Homeobox Protein Engrailed-2 (EN2) Antibody |
|||
abx033410-400ul | Abbexa | 400 ul | EUR 627.6 |
Homeobox Protein Engrailed-2 (EN2) Antibody |
|||
abx033410-80l | Abbexa | 80 µl | EUR 343.2 |
Homeobox Protein Engrailed-2 (EN2) Antibody |
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abx033411-400ul | Abbexa | 400 ul | EUR 627.6 |
Homeobox Protein Engrailed-2 (EN2) Antibody |
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abx033411-80l | Abbexa | 80 µl | EUR 343.2 |
Homeobox Protein Engrailed-2 (EN2) Antibody |
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20-abx302580 | Abbexa |
|
|
Homeobox Protein Engrailed-2 (EN2) Antibody |
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abx302580-100g | Abbexa | 100 µg | EUR 362.5 |
Homeobox Protein Engrailed-2 (EN2) Antibody |
|||
abx302580-20g | Abbexa | 20 µg | EUR 162.5 |
Homeobox Protein Engrailed-2 (EN2) Antibody |
|||
abx302580-50g | Abbexa | 50 µg | EUR 250 |
Homeobox Protein Engrailed-2 (EN2) Antibody |
|||
abx033410-100g | Abbexa | 100 µg | EUR 250 |
Homeobox Protein Engrailed-2 (EN2) Antibody |
|||
abx033411-100g | Abbexa | 100 µg | EUR 281.25 |
Homeobox Protein Engrailed-2 (EN2) Antibody (HRP) |
|||
20-abx315705 | Abbexa |
|
|
Homeobox Protein Engrailed-2 (EN2) Antibody (HRP) |
|||
abx315705-100g | Abbexa | 100 µg | EUR 362.5 |
Homeobox Protein Engrailed-2 (EN2) Antibody (HRP) |
|||
abx315705-20g | Abbexa | 20 µg | EUR 162.5 |
Homeobox Protein Engrailed-2 (EN2) Antibody (HRP) |
|||
abx315705-50g | Abbexa | 50 µg | EUR 250 |
Engrailed Homeobox Protein 2 (EN2) Antibody |
|||
20-abx172243 | Abbexa |
|
|
Engrailed Homeobox Protein 2 (EN2) Antibody |
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20-abx176276 | Abbexa |
|
|
Engrailed Homeobox Protein 2 (EN2) Antibody |
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20-abx176277 | Abbexa |
|
|
Engrailed Homeobox Protein 2 (EN2) Antibody |
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abx172243-1ml | Abbexa | 1 ml | EUR 787.5 |
Engrailed Homeobox Protein 2 (EN2) Antibody |
|||
abx176277-1096tests | Abbexa | 10 × 96 tests | EUR 825 |
Engrailed Homeobox Protein 2 (EN2) Antibody |
|||
abx176277-596tests | Abbexa | 5 × 96 tests | EUR 362.5 |
Engrailed Homeobox Protein 2 (EN2) Antibody |
|||
abx176277-96tests | Abbexa | 96 tests | EUR 287.5 |
Homeobox Protein Engrailed-2 (EN2) Antibody (FITC) |
|||
20-abx315706 | Abbexa |
|
|
Homeobox Protein Engrailed-2 (EN2) Antibody (FITC) |
|||
abx315706-100g | Abbexa | 100 µg | EUR 362.5 |
Homeobox Protein Engrailed-2 (EN2) Antibody (FITC) |
|||
abx315706-20g | Abbexa | 20 µg | EUR 162.5 |
Homeobox Protein Engrailed-2 (EN2) Antibody (FITC) |
|||
abx315706-50g | Abbexa | 50 µg | EUR 250 |
Human Engrailed Homeobox Protein 2 (EN2) Protein |
|||
20-abx652255 | Abbexa |
|
|
Human Engrailed Homeobox Protein 2 (EN2) Protein |
|||
abx652255-5mg | Abbexa | 5 mg | EUR 362.5 |
Human Homeobox protein engrailed- 2, EN2 ELISA KIT |
|||
ELI-05951h | Lifescience Market | 96 Tests | EUR 988.8 |
Mouse Homeobox protein engrailed- 2, En2 ELISA KIT |
|||
ELI-05952m | Lifescience Market | 96 Tests | EUR 1038 |
Homeobox Protein Engrailed-2 (EN2) Antibody (Biotin) |
|||
20-abx315707 | Abbexa |
|
|
Homeobox Protein Engrailed-2 (EN2) Antibody (Biotin) |
|||
abx315707-100g | Abbexa | 100 µg | EUR 362.5 |
Homeobox Protein Engrailed-2 (EN2) Antibody (Biotin) |
|||
abx315707-20g | Abbexa | 20 µg | EUR 162.5 |
Homeobox Protein Engrailed-2 (EN2) Antibody (Biotin) |
|||
abx315707-50g | Abbexa | 50 µg | EUR 250 |
Human Homeobox Protein Engrailed-2 (EN2) ELISA Kit |
|||
abx251286-96tests | Abbexa | 96 tests | EUR 801.6 |
Mouse Homeobox Protein Engrailed-2 (EN2) ELISA Kit |
|||
abx518617-96tests | Abbexa | 96 tests | EUR 801.6 |
Human Homeobox protein engrailed-2(EN2) ELISA kit |
|||
EKC41536-48T | Biomatik Corporation | 48T | EUR 535.99 |
Human Homeobox protein engrailed-2(EN2) ELISA kit |
|||
EKC41536-5x96T | Biomatik Corporation | 5x96T | EUR 3637.08 |
Human Homeobox protein engrailed-2(EN2) ELISA kit |
|||
EKC41536-96T | Biomatik Corporation | 96T | EUR 765.7 |
Human Homeobox protein engrailed-2(EN2) Elisa Kit |
|||
EK712182 | AFG Bioscience LLC | 96 Wells | EUR 0.24 |
Human Homeobox protein engrailed-2(EN2) ELISA kit |
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CSB-EL007660HU-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
Description: Quantitativesandwich ELISA kit for measuring Human Homeobox protein engrailed-2 (EN2) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human Homeobox protein engrailed-2(EN2) ELISA kit |
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1-CSB-EL007660HU | Cusabio |
|
|
Description: Quantitativesandwich ELISA kit for measuring Human Homeobox protein engrailed-2(EN2) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Mouse Homeobox protein engrailed-2 (EN2) ELISA Kit |
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RK08809 | Abclonal | 96T | EUR 280 |
Human Homeobox protein engrailed-2 (EN2) ELISA Kit |
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RK01311 | Abclonal | 96 Tests | EUR 280 |
Engrailed Homeobox 2 Protein (OVA) |
|||
20-abx165529 | Abbexa |
|
|
Human EN2(Homeobox protein engrailed-2) ELISA Kit |
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EH1964 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
Human EN2/ Homeobox protein engrailed-2 ELISA Kit |
|||
E0787Hu | Sunlong | 1 Kit | EUR 515 |
Human EN2(Homeobox protein engrailed-2) ELISA Kit |
|||
EKF57407-48T | Biomatik Corporation | 48T | EUR 396.9 |
Human EN2(Homeobox protein engrailed-2) ELISA Kit |
|||
EKF57407-5x96T | Biomatik Corporation | 5x96T | EUR 2693.25 |
Human EN2(Homeobox protein engrailed-2) ELISA Kit |
|||
EKF57407-96T | Biomatik Corporation | 96T | EUR 567 |
Human EN2 / Homeobox protein engrailed-2 ELISA Kit |
|||
E1851h | EIAab | 96T | EUR 736 |
Mouse En2 / Homeobox protein engrailed-2 ELISA Kit |
|||
E1851m | EIAab | 96T | EUR 776 |
Engrailed Homeobox Protein 2 (EN2) Antibody Pair |
|||
20-abx370677 | Abbexa |
|
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Engrailed Homeobox Protein 2 (EN2) Antibody Pair |
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abx370677-100g | Abbexa | 100 µg | EUR 2362.5 |
Engrailed Homeobox Protein 2 (EN2) Antibody Pair |
|||
abx370677-50g | Abbexa | 50 µg | EUR 1462.5 |
Human Homeobox Protein engrailed-2, EN2 GENLISA ELISA |
|||
KBH5427 | Krishgen | 1 x 96 wells | EUR 286 |
OVA Conjugated Engrailed Homeobox Protein 2 (EN2) |
|||
CPJ982Hu21 | Cloud-Clone | 10ug | EUR 140 |
OVA conjugated Engrailed Homeobox Protein 2 (EN2) |
|||
4-CPJ982Hu21 | Cloud-Clone |
|
|
Description: Recombinant Human Engrailed Homeobox Protein 2 expressed in: chemical synthesis |
OVA Conjugated Engrailed Homeobox Protein 2 (EN2) |
|||
RPU50907-100ug | Biomatik Corporation | 100ug | EUR 431.2 |
OVA Conjugated Engrailed Homeobox Protein 2 (EN2) |
|||
RPU50907-1mg | Biomatik Corporation | 1mg | EUR 1911 |
OVA Conjugated Engrailed Homeobox Protein 2 (EN2) |
|||
RPU50907-50ug | Biomatik Corporation | 50ug | EUR 336 |
Human Engrailed Homeobox Protein 2 (EN2) CLIA Kit |
|||
20-abx495807 | Abbexa |
|
|
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
20-abx151405 | Abbexa |
|
|
Human Engrailed Homeobox Protein 2 (EN2)ELISA Kit |
|||
201-12-2671 | SunredBio | 96 tests | EUR 528 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
abx571248-96tests | Abbexa | 96 tests | EUR 990 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
DLR-EN2-Hu | DL Develop | 96T | EUR 463 |
Description: tissue homogenates or other biological fluids. |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
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DLR-EN2-Hu-48T | DL Develop | 48T | EUR 620.4 |
Description: A sandwich quantitative ELISA assay kit for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates or other biological fluids. |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
DLR-EN2-Hu-96T | DL Develop | 96T | EUR 807.6 |
Description: A sandwich quantitative ELISA assay kit for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates or other biological fluids. |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
EKN45006-48T | Biomatik Corporation | 48T | EUR 378.7 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
EKN45006-5x96T | Biomatik Corporation | 5x96T | EUR 2569.75 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
EKN45006-96T | Biomatik Corporation | 96T | EUR 541 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
EKL57008-5x96T | Biomatik Corporation | 5x96T | EUR 3793.35 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
EKL57008-96T | Biomatik Corporation | 96T | EUR 798.6 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
EK13816 | SAB | 96Т | EUR 768 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
EKU03895-48T | Biomatik Corporation | 48T | EUR 555.66 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
EKU03895-5x96T | Biomatik Corporation | 5x96T | EUR 3770.55 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
EKU03895-96T | Biomatik Corporation | 96T | EUR 793.8 |
Human EN2(Engrailed Homeobox Protein 2) ELISA Kit |
|||
ELK3841-48T | ELK Biotech | 48T | Ask for price |
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EN2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Human EN2(Engrailed Homeobox Protein 2) ELISA Kit |
|||
ELK3841-96T | ELK Biotech | 96T | Ask for price |
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EN2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
DL-EN2-Hu | DL Develop | 96T | EUR 441 |
Description: tissue homogenates or other biological fluids. |
Human Engrailed Homeobox Protein 2(EN2)ELISA Kit |
|||
QY-E04943 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
RDR-EN2-Hu-48T | Reddot Biotech | 48T | EUR 475.34 |
Description: tissue homogenates and other biological fluids. |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
RDR-EN2-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 652.8 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
RDR-EN2-Hu-96T | Reddot Biotech | 96T | EUR 679.04 |
Description: tissue homogenates and other biological fluids. |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
RDR-EN2-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 907.2 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
RD-EN2-Hu-48T | Reddot Biotech | 48T | EUR 452.7 |
Description: tissue homogenates and other biological fluids. |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
RD-EN2-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 625.2 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
RD-EN2-Hu-96T | Reddot Biotech | 96T | EUR 646.7 |
Description: tissue homogenates and other biological fluids. |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
RD-EN2-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 867.6 |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
SEJ982Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 5677.8 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids. |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
SEJ982Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 572.76 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids. |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
SEJ982Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 766.8 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids. |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
SEJ982Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3090.6 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Engrailed Homeobox Protein 2 (EN2) in tissue homogenates and other biological fluids. |
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit |
|||
4-SEJ982Hu | Cloud-Clone |
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Engrailed Homeobox Protein 2 (EN2) in samples from tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. |
Human Engrailed Homeobox Protein 2 (EN2)ELISA Kit |
|||
YLA3312HU-48T | Shanghai YL Biotech | 48T | Ask for price |
Human Engrailed Homeobox Protein 2 (EN2)ELISA Kit |
|||
YLA3312HU-96T | Shanghai YL Biotech | 96T | Ask for price |
Lenti ORF clone of Human engrailed homeobox 1 (EN1), mGFP tagged |
|||
RC224659L4 | Origene Technologies GmbH | 10 µg | Ask for price |
Engrailed Homeobox Protein 2 (EN2) Polyclonal Antibody |
|||
CAU31880-100ul | Biomatik Corporation | 100ul | EUR 258.2 |
Engrailed Homeobox Protein 2 (EN2) Polyclonal Antibody |
|||
CAU31880-200ul | Biomatik Corporation | 200ul | EUR 322.6 |
Human Engrailed Homeobox Protein 2, EN2 GENLISA ELISA |
|||
KBH2670 | Krishgen | 1 x 96 wells | EUR 286 |
ELISA kit for Human EN2 (Engrailed Homeobox Protein 2) |
|||
ELK3841 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Description: A sandwich ELISA kit for detection of Engrailed Homeobox Protein 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Human Homeobox protein engrailed-2 ELISA Kit |
|||
EK4019 | SAB | 96 tests | EUR 599 |
Lenti ORF particles, EN1 (mGFP-tagged) - Human engrailed homeobox 1 (EN1), 200ul, >10^7 TU/mL |
|||
RC224659L4V | Origene Technologies GmbH | 200 µl | Ask for price |
Rat Engrailed Homeobox Protein 2 ELISA kit |
|||
E02E0151-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Engrailed Homeobox Protein 2 ELISA kit |
|||
E02E0151-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Engrailed Homeobox Protein 2 ELISA kit |
|||
E02E0151-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Rat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Engrailed Homeobox Protein 2 ELISA kit |
|||
E07E0151-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Engrailed Homeobox Protein 2 ELISA kit |
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E07E0151-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Engrailed Homeobox Protein 2 ELISA kit |
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E07E0151-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Porcine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Engrailed Homeobox Protein 2 ELISA kit |
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E08E0151-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Engrailed Homeobox Protein 2 ELISA kit |
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E08E0151-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Engrailed Homeobox Protein 2 ELISA kit |
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E08E0151-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Canine Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Engrailed Homeobox Protein 2 ELISA kit |
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E01A13042 | BlueGene | 96T | EUR 700 |
Description: ELISA |
Goat Engrailed Homeobox Protein 2 ELISA kit |
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E01A47951 | BlueGene | 96T | EUR 700 |
Description: ELISA |
Goat Engrailed Homeobox Protein 2 ELISA kit |
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E06E0151-192T | BlueGene | 192 tests | EUR 1524 |
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Engrailed Homeobox Protein 2 ELISA kit |
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E06E0151-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Engrailed Homeobox Protein 2 ELISA kit |
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E06E0151-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A sandwich ELISA for quantitative measurement of Goat Engrailed Homeobox Protein 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Lenti ORF clone of Human engrailed homeobox 1 (EN1), Myc-DDK-tagged |
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RC224659L3 | Origene Technologies GmbH | 10 µg | Ask for price |
Mouse Engrailed Homeobox Protein 2 ELISA kit |
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E01A21782 | BlueGene | 96T | EUR 700 |
Description: ELISA |
A protein fragment of Rv3194c positioned on mycobacterial cell flooring successfully prevents adhesion of recombinant Mycobacterium smegmatis, and ensures a model new anti-adhesive drug
Adhesins are virulence components expressed on the surfaces of pathogenic micro organism that mediate pathogen-host interactions, a vital step throughout the an an infection course of.
Proper right here, we current that the Mycobacterium tuberculosis protease Rv3194c capabilities not solely as an enzyme nevertheless as an adhesin. The heterologous Rv3194c protein was purified from Escherichia coli and was confirmed to bind to hyaluronic acid (HA).
The HA-binding website was acknowledged as a 20 amino acid peptide between residues 91 and 110 (P91-110). Rv3194c certain to A549 alveolar basal epithelial cells and the interaction was abolished by the addition of hyaluronidase or P91-110.
Experimental an an infection in vitro revealed that Rv3194c participates throughout the attachment of recombinant Mycobacterium smegmatis (Rv3194c/MS) to A549 cells, and P91-110 treatment of A549 cells largely inhibited the Rv3194c/MS-A549 cell interaction.
To supply in vivo proof, we constructed a reporter stress of M. smegmatis that expressed a by-product of the firefly luciferase that is shifted to pink (FFlucRT) along with Rv3194c (Rv3194c + FFlucRT/MS) to infect mice and monitor the event of the sickness.
In mice, Rv3194c dramatically enhanced M. smegmatis persistence and induced lesions throughout the lungs. In addition to, treatment of intratracheal Rv3194c + FFlucRT/MS- contaminated mice with P91-110 significantly suppressed the growth of Rv3194c + FFlucRT/MS in vivo and diminished pathological injury introduced on by an an infection of the lung with Rv3194c + FFlucRT/MS.
Taken collectively, these outcomes reveal that Rv3194c capabilities as an HA-binding adhesin and that P91-110 may need the potential for treating and stopping mycobacterial an an infection.
Lysine acylation using conjugating enzymes for site-specific modification and ubiquitination of recombinantproteins
Enzymes are extremely efficient devices for protein labelling attributable to their specificity and delicate response conditions. Many protocols, nonetheless, are restricted to modifications at protein termini, depend upon non-peptidic metabolites or require large recognition domains. Proper right here we report a chemoenzymatic methodology, which we title lysine acylation using conjugating enzymes (LACE), to site-specifically modify folded proteins at inside lysine residues. LACE depends upon a minimal genetically encoded tag (Four residues) acknowledged by the E2 small ubiquitin-like modifier-conjugating enzyme Ubc9, and peptide or protein thioesters.
Collectively, this methodology obviates the need for E1 and E3 enzymes, enabling isopeptide formation with merely Ubc9 in a programmable methodology. We reveal the utility of LACE by the site-specific attachment of biochemical probes, one-pot dual-labelling collectively with sortase, and the conjugation of wild-type ubiquitin and ISG15 to recombinant proteins.
Antifungal Protein (AFP) Antibody (HRP) |
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20-abx333617 | Abbexa |
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Antifungal Protein (AFP) Antibody (HRP) |
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abx333617-100g | Abbexa | 100 µg | EUR 362.5 |
Antifungal Protein (AFP) Antibody (HRP) |
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abx333617-20g | Abbexa | 20 µg | EUR 162.5 |
Antifungal Protein (AFP) Antibody (HRP) |
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abx333617-50g | Abbexa | 50 µg | EUR 250 |
Antifungal Protein (AFP) Antibody (FITC) |
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20-abx333618 | Abbexa |
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Antifungal Protein (AFP) Antibody (FITC) |
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abx333618-100g | Abbexa | 100 µg | EUR 362.5 |
Antifungal Protein (AFP) Antibody (FITC) |
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abx333618-20g | Abbexa | 20 µg | EUR 162.5 |
Antifungal Protein (AFP) Antibody (FITC) |
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abx333618-50g | Abbexa | 50 µg | EUR 250 |
Antifungal Protein (AFP) Antibody (Biotin) |
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20-abx333619 | Abbexa |
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Antifungal Protein (AFP) Antibody (Biotin) |
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abx333619-100g | Abbexa | 100 µg | EUR 362.5 |
Antifungal Protein (AFP) Antibody (Biotin) |
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abx333619-20g | Abbexa | 20 µg | EUR 162.5 |
Antifungal Protein (AFP) Antibody (Biotin) |
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abx333619-50g | Abbexa | 50 µg | EUR 250 |
Antifungal agent 1 |
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HY-102025 | MedChemExpress | 50mg | EUR 2664 |
Antifungal agent 20 |
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T40124-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 20 |
Antifungal agent 20 |
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T40124-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 20 |
Antifungal agent 20 |
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T40124-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 20 |
Antifungal agent 20 |
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T40124-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 20 |
Antifungal agent 20 |
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T40124-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 20 |
Antifungal agent 15 |
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T40262-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 15 |
Antifungal agent 15 |
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T40262-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 15 |
Antifungal agent 15 |
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T40262-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 15 |
Antifungal agent 15 |
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T40262-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 15 |
Antifungal agent 15 |
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T40262-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 15 |
Antifungal agent 18 |
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T40208-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 18 |
Antifungal agent 18 |
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T40208-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 18 |
Antifungal agent 18 |
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T40208-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 18 |
Antifungal agent 18 |
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T40208-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 18 |
Antifungal agent 18 |
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T40208-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 18 |
Antifungal agent 14 |
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T40329-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 14 |
Antifungal agent 14 |
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T40329-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 14 |
Antifungal agent 14 |
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T40329-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 14 |
Antifungal agent 14 |
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T40329-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 14 |
Antifungal agent 14 |
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T40329-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 14 |
Antifungal agent 1 |
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T10333-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 1 |
Antifungal agent 1 |
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T10333-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 1 |
Antifungal agent 1 |
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T10333-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 1 |
Antifungal agent 1 |
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T10333-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 1 |
Antifungal agent 1 |
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T10333-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 1 |
Antifungal agent 2 |
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T10334-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 2 |
Antifungal agent 2 |
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T10334-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 2 |
Antifungal agent 2 |
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T10334-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 2 |
Antifungal agent 2 |
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T10334-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 2 |
Antifungal agent 2 |
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T10334-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 2 |
Antifungal agent 25 |
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T64235-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 25 |
Antifungal agent 25 |
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T64235-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 25 |
Antifungal agent 25 |
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T64235-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 25 |
Antifungal agent 25 |
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T64235-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 25 |
Antifungal agent 25 |
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T64235-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 25 |
Antifungal agent 38 |
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T60236-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 38 |
Antifungal agent 38 |
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T60236-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 38 |
Antifungal agent 38 |
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T60236-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 38 |
Antifungal agent 38 |
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T60236-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 38 |
Antifungal agent 38 |
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T60236-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 38 |
Antifungal agent 36 |
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T60320-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 36 |
Antifungal agent 36 |
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T60320-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 36 |
Antifungal agent 36 |
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T60320-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 36 |
Antifungal agent 36 |
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T60320-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 36 |
Antifungal agent 36 |
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T60320-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 36 |
Antifungal agent 28 |
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T62098-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 28 |
Antifungal agent 28 |
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T62098-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 28 |
Antifungal agent 28 |
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T62098-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 28 |
Antifungal agent 28 |
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T62098-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 28 |
Antifungal agent 28 |
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T62098-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 28 |
Antifungal agent 24 |
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T62159-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 24 |
Antifungal agent 24 |
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T62159-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 24 |
Antifungal agent 24 |
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T62159-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 24 |
Antifungal agent 24 |
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T62159-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 24 |
Antifungal agent 24 |
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T62159-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 24 |
Antifungal agent 33 |
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T62211-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 33 |
Antifungal agent 33 |
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T62211-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 33 |
Antifungal agent 33 |
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T62211-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 33 |
Antifungal agent 33 |
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T62211-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 33 |
Antifungal agent 33 |
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T62211-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 33 |
Antifungal agent 17 |
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T62280-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 17 |
Antifungal agent 17 |
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T62280-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 17 |
Antifungal agent 17 |
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T62280-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 17 |
Antifungal agent 17 |
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T62280-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 17 |
Antifungal agent 17 |
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T62280-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 17 |
Antifungal agent 16 |
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T63157-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 16 |
Antifungal agent 16 |
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T63157-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 16 |
Antifungal agent 16 |
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T63157-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 16 |
Antifungal agent 16 |
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T63157-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 16 |
Antifungal agent 16 |
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T63157-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 16 |
Antifungal agent 40 |
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T63285-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 40 |
Antifungal agent 40 |
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T63285-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 40 |
Antifungal agent 40 |
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T63285-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Antifungal agent 40 |
Antifungal agent 40 |
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T63285-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Antifungal agent 40 |
Antifungal agent 40 |
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T63285-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Antifungal agent 40 |
Antifungal agent 31 |
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T63314-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Antifungal agent 31 |
Antifungal agent 31 |
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T63314-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Antifungal agent 31 |