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fragment of Rv3194c positioned on mycobacterial cell flooring successfully prevents adhesion of recombinant Mycobacterium smegmatis, and ensures a model new

Microscale Perfusion-based Cultivation for Pichia pastoris Clone Screening Enables Accelerated and Optimized RecombinantProtein Production Processes

Pichia pastoris has emerged before now years as a promising host for recombinant protein and biopharmaceutical manufacturing. Inside the establishment of extreme cell density fed-batch biomanufacturing, screening half and early bioprocess progress (based totally on microplates and shake flasks) nonetheless characterize a bottleneck attributable to high-cost and time-consuming procedures along with low experiment complexity.

 Inside the present work, we implement a screening protocol developed for P. pastoris clone alternative in a multiplexed microfluidic machine with 15 μL cultivation chambers ready to operate in perfusion mode and monitor dissolved oxygen content material materials throughout the custom in a non-invasive means.

The setup allowed us to establish carbon-limited conditions and think about stress responses to completely completely different enter variables. Outcomes from micro-scale perfusion cultures had been then in distinction with 1L fed-batch fermentation.

The simplest producer by the use of titer and productiveness was shortly acknowledged after 12 hours from inoculation and the outcomes confirmed by lab-scale fermentation.

Moreover, the physiological analyses of the strains beneath completely completely different conditions steered how further sophisticated experimental conditions had been achievable whatever the comparatively easy, straight-forward, and cost-effective experimental setup.

Implementation and standardization of these micro-scale protocols might in the reduction of the demand for lab-scale bioreactor cultivations thus accelerating the occasion of protein manufacturing processes. This textual content is protected by copyright. All rights reserved.

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aabioetica

Recombinant Chicken Homeobox protein engrailed-1 (EN1)

MBS948673-01mgEColi 0.1mg(E-Coli)
EUR 635

Recombinant Chicken Homeobox protein engrailed-1 (EN1)

MBS948673-5x01mgEColi 5x0.1mg(E-Coli)
EUR 2630

Chicken Homeobox protein engrailed-1 (EN1)

1-CSB-EP007659CH
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  • 50ug
Description: Recombinant Chicken Homeobox protein engrailed-1(EN1) expressed in E.coli

Chicken Homeobox protein engrailed- 1, EN1 ELISA KIT

ELI-48109c 96tests
EUR 796

Chicken Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9350982-INQUIRE INQUIRE Ask for price

Chicken Homeobox protein engrailed-1, EN1 ELISA Kit

MBS9318877-INQUIRE INQUIRE Ask for price

Recombinant Mouse Homeobox protein engrailed-1 (En1)

MBS967320-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1275

Recombinant Mouse Homeobox protein engrailed-1 (En1)

MBS967320-002mgEColi 0.02mg(E-Coli)
EUR 945

Recombinant Mouse Homeobox protein engrailed-1 (En1)

MBS967320-002mgYeast 0.02mg(Yeast)
EUR 1065

Recombinant Mouse Homeobox protein engrailed-1 (En1)

MBS967320-01mgEColi 0.1mg(E-Coli)
EUR 1140

Recombinant Mouse Homeobox protein engrailed-1 (En1)

MBS967320-01mgYeast 0.1mg(Yeast)
EUR 1215

Recombinant Human Homeobox protein engrailed-1 (EN1)

MBS963313-INQUIRE INQUIRE Ask for price

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-1mg

QP5983-ec-1mg 1mg
EUR 2763.6

Recombinant Human Homeobox protein engrailed-1 (EN1), partial

MBS964664-002mgBaculovirus 0.02mg(Baculovirus)
EUR 990

Recombinant Human Homeobox protein engrailed-1 (EN1), partial

MBS964664-002mgEColi 0.02mg(E-Coli)
EUR 555

Recombinant Human Homeobox protein engrailed-1 (EN1), partial

MBS964664-002mgYeast 0.02mg(Yeast)
EUR 745

Recombinant Human Homeobox protein engrailed-1 (EN1), partial

MBS964664-01mgEColi 0.1mg(E-Coli)
EUR 660

Recombinant Human Homeobox protein engrailed-1 (EN1), partial

MBS964664-01mgYeast 0.1mg(Yeast)
EUR 870

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-100ug

QP5983-ec-100ug 100ug
EUR 848.4

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-10ug

QP5983-ec-10ug 10ug
EUR 391.2

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-200ug

QP5983-ec-200ug 200ug
EUR 1338

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-500ug

QP5983-ec-500ug 500ug
EUR 1816.8

Recombinant Chicken Homeobox protein engrailed-1 Protein, His-B2M, E.coli-50ug

QP5983-ec-50ug 50ug
EUR 522

Chicken Homeobox Protein Engrailed-2 (EN2) ELISA Kit

abx518615-96tests 96 tests
EUR 1093.2

Chicken Homeobox protein engrailed-2,EN2 ELISA KIT

ELI-05953c 96tests
EUR 796

Chicken Homeobox protein engrailed-2, EN2 ELISA Kit

MBS9343084-INQUIRE INQUIRE Ask for price

Chicken EN2 / Homeobox protein engrailed-2 ELISA Kit

E1851c 96T
EUR 900

Chicken Homeobox protein engrailed-2 ELISA Kit

MBS289843-10x96StripWells 10x96-Strip-Wells
EUR 4630

Chicken Homeobox protein engrailed-2 ELISA Kit

MBS289843-48StripWells 48-Strip-Wells
EUR 440

Chicken Homeobox protein engrailed-2 ELISA Kit

MBS289843-5x96StripWells 5x96-Strip-Wells
EUR 2480

Chicken Homeobox protein engrailed-2 ELISA Kit

MBS289843-96StripWells 96-Strip-Wells
EUR 580

Homeobox Protein Engrailed-1 (EN1) Antibody

abx033297-400ul 400 ul
EUR 627.6

Homeobox Protein Engrailed-1 (EN1) Antibody

abx033297-80l 80 µl
EUR 343.2

Homeobox Protein Engrailed-1 (EN1) Antibody

20-abx215150
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Homeobox Protein Engrailed-1 (EN1) Antibody

20-abx300471
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Homeobox Protein Engrailed-1 (EN1) Antibody

abx033297-100g 100 µg
EUR 281.25

Homeobox Protein Engrailed-1 (EN1) Antibody

abx300471-100g 100 µg
EUR 362.5

Homeobox Protein Engrailed-1 (EN1) Antibody

abx300471-20g 20 µg
EUR 162.5

Homeobox Protein Engrailed-1 (EN1) Antibody

abx300471-50g 50 µg
EUR 250

Chicken Engrailed Homeobox Protein 2 ELISA kit

E01A91538 96T
EUR 700
Description: ELISA

Chicken Engrailed Homeobox Protein 2 ELISA Kit

MBS038008-INQUIRE INQUIRE Ask for price

Chicken Engrailed Homeobox Protein 2 ELISA Kit

MBS743330-10x96StripWells 10x96-Strip-Wells
EUR 5685

Chicken Engrailed Homeobox Protein 2 ELISA Kit

MBS743330-48StripWells 48-Strip-Wells
EUR 485

Chicken Engrailed Homeobox Protein 2 ELISA Kit

MBS743330-5x96StripWells 5x96-Strip-Wells
EUR 3020

Chicken Engrailed Homeobox Protein 2 ELISA Kit

MBS743330-96StripWells 96-Strip-Wells
EUR 690

Chicken Engrailed Homeobox Protein 2 ELISA Kit

MBS747724-10x96StripWells 10x96-Strip-Wells
EUR 5685

Chicken Engrailed Homeobox Protein 2 ELISA Kit

MBS747724-48StripWells 48-Strip-Wells
EUR 485

Chicken Engrailed Homeobox Protein 2 ELISA Kit

MBS747724-5x96StripWells 5x96-Strip-Wells
EUR 3020

Chicken Engrailed Homeobox Protein 2 ELISA Kit

MBS747724-96StripWells 96-Strip-Wells
EUR 690

Homeobox Protein Engrailed-1 (EN1) Antibody (HRP)

20-abx300472
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  • 100 ug
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  • 200 ug
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  • 50 ug

Homeobox Protein Engrailed-1 (EN1) Antibody (HRP)

abx300472-100g 100 µg
EUR 362.5

Homeobox Protein Engrailed-1 (EN1) Antibody (HRP)

abx300472-20g 20 µg
EUR 162.5

Homeobox Protein Engrailed-1 (EN1) Antibody (HRP)

abx300472-50g 50 µg
EUR 250

Homeobox Protein Engrailed-1 (EN1) Antibody (FITC)

20-abx300473
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Homeobox Protein Engrailed-1 (EN1) Antibody (FITC)

abx300473-100g 100 µg
EUR 362.5

Homeobox Protein Engrailed-1 (EN1) Antibody (FITC)

abx300473-20g 20 µg
EUR 162.5

Homeobox Protein Engrailed-1 (EN1) Antibody (FITC)

abx300473-50g 50 µg
EUR 250

Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin)

20-abx300474
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  • 100 ug
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Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin)

abx300474-100g 100 µg
EUR 362.5

Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin)

abx300474-20g 20 µg
EUR 162.5

Homeobox Protein Engrailed-1 (EN1) Antibody (Biotin)

abx300474-50g 50 µg
EUR 250

Cat Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9360620-INQUIRE INQUIRE Ask for price

Rat Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9355366-10x96StripWells 10x96-Strip-Wells
EUR 6725

Rat Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9355366-48StripWells 48-Strip-Wells
EUR 550

Rat Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9355366-5x96StripWells 5x96-Strip-Wells
EUR 3420

Rat Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9355366-96StripWells 96-Strip-Wells
EUR 765

Mouse Homeobox protein engrailed- 1, En1 ELISA KIT

ELI-27118m 96tests
EUR 736

Human Homeobox protein engrailed- 1, EN1 ELISA KIT

ELI-48646h 96tests
EUR 696

Duck Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9367960-INQUIRE INQUIRE Ask for price

Goat Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9346657-INQUIRE INQUIRE Ask for price

Cavy Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9353886-INQUIRE INQUIRE Ask for price

Fish Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9360973-INQUIRE INQUIRE Ask for price

Human Homeobox protein engrailed-1 (EN1) ELISA Kit

abx527211-96tests 96 tests
EUR 687.5

Mouse Homeobox protein engrailed-1 (EN1) ELISA Kit

abx527212-96tests 96 tests
EUR 687.5

Goose Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9363367-INQUIRE INQUIRE Ask for price

Horse Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9370113-10x96StripWells 10x96-Strip-Wells
EUR 6725

Horse Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9370113-48StripWells 48-Strip-Wells
EUR 550

Horse Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9370113-5x96StripWells 5x96-Strip-Wells
EUR 3420

Horse Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9370113-96StripWells 96-Strip-Wells
EUR 765

Mouse Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9370190-10x96StripWells 10x96-Strip-Wells
EUR 6725

Mouse Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9370190-48StripWells 48-Strip-Wells
EUR 550

Mouse Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9370190-5x96StripWells 5x96-Strip-Wells
EUR 3420

Mouse Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9370190-96StripWells 96-Strip-Wells
EUR 765

Camel Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9355823-10x96StripWells 10x96-Strip-Wells
EUR 6725

Camel Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9355823-48StripWells 48-Strip-Wells
EUR 550

Camel Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9355823-5x96StripWells 5x96-Strip-Wells
EUR 3420

Camel Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9355823-96StripWells 96-Strip-Wells
EUR 765

Human Homeobox protein engrailed-1, EN1 ELISA Kit

MBS9333441-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human Homeobox protein engrailed-1, EN1 ELISA Kit

MBS9333441-48StripWells 48-Strip-Wells
EUR 550

Human Homeobox protein engrailed-1, EN1 ELISA Kit

MBS9333441-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human Homeobox protein engrailed-1, EN1 ELISA Kit

MBS9333441-96StripWells 96-Strip-Wells
EUR 765

Mouse Homeobox protein engrailed-1, EN1 ELISA Kit

MBS9332208-10x96StripWells 10x96-Strip-Wells
EUR 6725

Mouse Homeobox protein engrailed-1, EN1 ELISA Kit

MBS9332208-48StripWells 48-Strip-Wells
EUR 550

Mouse Homeobox protein engrailed-1, EN1 ELISA Kit

MBS9332208-5x96StripWells 5x96-Strip-Wells
EUR 3420

Mouse Homeobox protein engrailed-1, EN1 ELISA Kit

MBS9332208-96StripWells 96-Strip-Wells
EUR 765

Plant Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9373662-INQUIRE INQUIRE Ask for price

Sheep Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9344918-10x96StripWells 10x96-Strip-Wells
EUR 6725

Sheep Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9344918-48StripWells 48-Strip-Wells
EUR 550

Sheep Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9344918-5x96StripWells 5x96-Strip-Wells
EUR 3420

Sheep Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9344918-96StripWells 96-Strip-Wells
EUR 765

Donkey Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9357848-INQUIRE INQUIRE Ask for price

Canine Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9358432-10x96StripWells 10x96-Strip-Wells
EUR 6725

Canine Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9358432-48StripWells 48-Strip-Wells
EUR 550

Canine Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9358432-5x96StripWells 5x96-Strip-Wells
EUR 3420

Canine Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9358432-96StripWells 96-Strip-Wells
EUR 765

Rabbit Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9365805-INQUIRE INQUIRE Ask for price

Monkey Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9366332-10x96StripWells 10x96-Strip-Wells
EUR 6725

Monkey Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9366332-48StripWells 48-Strip-Wells
EUR 550

Monkey Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9366332-5x96StripWells 5x96-Strip-Wells
EUR 3420

Monkey Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9366332-96StripWells 96-Strip-Wells
EUR 765

Bovine Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9369454-10x96StripWells 10x96-Strip-Wells
EUR 6725

Bovine Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9369454-48StripWells 48-Strip-Wells
EUR 550

Bovine Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9369454-5x96StripWells 5x96-Strip-Wells
EUR 3420

Bovine Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9369454-96StripWells 96-Strip-Wells
EUR 765

Pigeon Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9346412-INQUIRE INQUIRE Ask for price

EN1 (engrailed Homeobox 1) (PE)

MBS6186677-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (PE)

MBS6186677-5x01mL 5x0.1mL
EUR 3800

EN1 (engrailed Homeobox 1) (AP)

MBS6162544-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (AP)

MBS6162544-5x01mL 5x0.1mL
EUR 3800

EN1 (engrailed Homeobox 1) (AP)

MBS6162545-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (AP)

MBS6162545-5x01mL 5x0.1mL
EUR 3800

EN1 (engrailed Homeobox 1) (AP)

MBS6164627-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (AP)

MBS6164627-5x01mL 5x0.1mL
EUR 3800

EN1 (engrailed Homeobox 1) (PE)

MBS6184594-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (PE)

MBS6184594-5x01mL 5x0.1mL
EUR 3800

EN1 (engrailed Homeobox 1) (PE)

MBS6184595-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (PE)

MBS6184595-5x01mL 5x0.1mL
EUR 3800

Porcine Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9362947-10x96StripWells 10x96-Strip-Wells
EUR 6725

Porcine Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9362947-48StripWells 48-Strip-Wells
EUR 550

Porcine Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9362947-5x96StripWells 5x96-Strip-Wells
EUR 3420

Porcine Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9362947-96StripWells 96-Strip-Wells
EUR 765

Hamster Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9344932-10x96StripWells 10x96-Strip-Wells
EUR 6725

Hamster Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9344932-48StripWells 48-Strip-Wells
EUR 550

Hamster Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9344932-5x96StripWells 5x96-Strip-Wells
EUR 3420

Hamster Homeobox Protein Engrailed-1 (EN1) ELISA Kit

MBS9344932-96StripWells 96-Strip-Wells
EUR 765

EN1 (engrailed Homeobox 1) (HRP)

MBS6182267-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (HRP)

MBS6182267-5x01mL 5x0.1mL
EUR 3800

EN1 (engrailed Homeobox 1) (HRP)

MBS6180184-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (HRP)

MBS6180184-5x01mL 5x0.1mL
EUR 3800

EN1 (engrailed Homeobox 1) (HRP)

MBS6180185-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (HRP)

MBS6180185-5x01mL 5x0.1mL
EUR 3800

EN1 (engrailed Homeobox 1) (APC)

MBS6169037-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (APC)

MBS6169037-5x01mL 5x0.1mL
EUR 3800

EN1 (engrailed Homeobox 1) (APC)

MBS6166954-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (APC)

MBS6166954-5x01mL 5x0.1mL
EUR 3800

EN1 (engrailed Homeobox 1) (APC)

MBS6166955-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (APC)

MBS6166955-5x01mL 5x0.1mL
EUR 3800

EN1 (engrailed Homeobox 1) (FITC)

MBS6177857-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (FITC)

MBS6177857-5x01mL 5x0.1mL
EUR 3800

EN1 (engrailed Homeobox 1) (FITC)

MBS6175774-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (FITC)

MBS6175774-5x01mL 5x0.1mL
EUR 3800

EN1 (engrailed Homeobox 1) (FITC)

MBS6175775-01mL 0.1(mL
EUR 875

EN1 (engrailed Homeobox 1) (FITC)

MBS6175775-5x01mL 5x0.1mL
EUR 3800

EN1 (Homeobox Protein Engrailed-1, Homeobox Protein En-1, Hu-En-1) (AP)

MBS6131118-01mL 0.1(mL
EUR 875

EN1 (Homeobox Protein Engrailed-1, Homeobox Protein En-1, Hu-En-1) (AP)

MBS6131118-5x01mL 5x0.1mL
EUR 3800

EN1 (Homeobox Protein Engrailed-1, Homeobox Protein En-1, Hu-En-1) (PE)

MBS6157633-01mL 0.1(mL
EUR 875

EN1 (Homeobox Protein Engrailed-1, Homeobox Protein En-1, Hu-En-1) (PE)

MBS6157633-5x01mL 5x0.1mL
EUR 3800

EN1 (Homeobox Protein Engrailed-1, Homeobox Protein En-1, Hu-En-1) APC

MBS6136421-01mL 0.1(mL
EUR 875

EN1 (Homeobox Protein Engrailed-1, Homeobox Protein En-1, Hu-En-1) APC

MBS6136421-5x01mL 5x0.1mL
EUR 3800

EN1 (Homeobox Protein Engrailed-1, Homeobox Protein En-1, Hu-En-1) (HRP)

MBS6152330-01mL 0.1(mL
EUR 875

A protein fragment of Rv3194c positioned on mycobacterial cell flooring successfully prevents adhesion of recombinant Mycobacterium smegmatis, and ensures a model new anti-adhesive drug

Adhesins are virulence components expressed on the surfaces of pathogenic micro organism that mediate pathogen-host interactions, a vital step throughout the an an infection course of.

Proper right here, we current that the Mycobacterium tuberculosis protease Rv3194c capabilities not solely as an enzyme nevertheless as an adhesin. The heterologous Rv3194c protein was purified from Escherichia coli and was confirmed to bind to hyaluronic acid (HA).

The HA-binding website was acknowledged as a 20 amino acid peptide between residues 91 and 110 (P91-110). Rv3194c certain to A549 alveolar basal epithelial cells and the interaction was abolished by the addition of hyaluronidase or P91-110.

Experimental an an infection in vitro revealed that Rv3194c participates throughout the attachment of recombinant Mycobacterium smegmatis (Rv3194c/MS) to A549 cells, and P91-110 treatment of A549 cells largely inhibited the Rv3194c/MS-A549 cell interaction.

To supply in vivo proof, we constructed a reporter stress of M. smegmatis that expressed a by-product of the firefly luciferase that is shifted to pink (FFlucRT) along with Rv3194c (Rv3194c + FFlucRT/MS) to infect mice and monitor the event of the sickness.

In mice, Rv3194c dramatically enhanced M. smegmatis persistence and induced lesions throughout the lungs. In addition to, treatment of intratracheal Rv3194c + FFlucRT/MS- contaminated mice with P91-110 significantly suppressed the growth of Rv3194c + FFlucRT/MS in vivo and diminished pathological injury introduced on by an an infection of the lung with Rv3194c + FFlucRT/MS.

Taken collectively, these outcomes reveal that Rv3194c capabilities as an HA-binding adhesin and that P91-110 may need the potential for treating and stopping mycobacterial an an infection.

Lysine acylation using conjugating enzymes for site-specific modification and ubiquitination of recombinantproteins

Enzymes are extremely efficient devices for protein labelling attributable to their specificity and delicate response conditions. Many protocols, nonetheless, are restricted to modifications at protein termini, depend upon non-peptidic metabolites or require large recognition domains. Proper right here we report a chemoenzymatic methodology, which we title lysine acylation using conjugating enzymes (LACE), to site-specifically modify folded proteins at inside lysine residues. LACE depends upon a minimal genetically encoded tag (Four residues) acknowledged by the E2 small ubiquitin-like modifier-conjugating enzyme Ubc9, and peptide or protein thioesters.

Collectively, this methodology obviates the need for E1 and E3 enzymes, enabling isopeptide formation with merely Ubc9 in a programmable methodology. We reveal the utility of LACE by the site-specific attachment of biochemical probes, one-pot dual-labelling collectively with sortase, and the conjugation of wild-type ubiquitin and ISG15 to recombinant proteins.

Recombinant Aspergillus giganteus Antifungal protein (afp)

RPC20359-100ug 100ug
EUR 721.6

Recombinant Aspergillus giganteus Antifungal protein (afp)

RPC20359-1mg 1mg
EUR 2884.7

Recombinant Aspergillus giganteus Antifungal protein (afp)

RPC20359-20ug 20ug
EUR 448.1

Recombinant Penicillium chrysogenum Antifungal protein (afp)

MBS1048409-002mgBaculovirus 0.02mg(Baculovirus)
EUR 985

Recombinant Penicillium chrysogenum Antifungal protein (afp)

MBS1048409-002mgEColi 0.02mg(E-Coli)
EUR 545

Recombinant Penicillium chrysogenum Antifungal protein (afp)

MBS1048409-002mgYeast 0.02mg(Yeast)
EUR 735

Recombinant Penicillium chrysogenum Antifungal protein (afp)

MBS1048409-01mgEColi 0.1mg(E-Coli)
EUR 650

Recombinant Penicillium chrysogenum Antifungal protein (afp)

MBS1048409-01mgYeast 0.1mg(Yeast)
EUR 860

Recombinant Penicillium chrysogenum Antifungal protein (afp)

MBS1153080-002mgBaculovirus 0.02mg(Baculovirus)
EUR 985

Recombinant Penicillium chrysogenum Antifungal protein (afp)

MBS1153080-002mgEColi 0.02mg(E-Coli)
EUR 545

Recombinant Penicillium chrysogenum Antifungal protein (afp)

MBS1153080-002mgYeast 0.02mg(Yeast)
EUR 735

Recombinant Penicillium chrysogenum Antifungal protein (afp)

MBS1153080-01mgEColi 0.1mg(E-Coli)
EUR 650

Recombinant Penicillium chrysogenum Antifungal protein (afp)

MBS1153080-01mgYeast 0.1mg(Yeast)
EUR 860

Antifungal protein (afp)

1-CSB-EP325933APN
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Antifungal protein(afp) expressed in E.coli

Antifungal Protein (AFP) Antibody

20-abx333616
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Antifungal Protein (AFP) Antibody

abx333616-100g 100 µg
EUR 362.5

Antifungal Protein (AFP) Antibody

abx333616-20g 20 µg
EUR 162.5

Antifungal Protein (AFP) Antibody

abx333616-50g 50 µg
EUR 250

Antifungal Protein (AFP) Antibody (HRP)

20-abx333617
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Antifungal Protein (AFP) Antibody (HRP)

abx333617-100g 100 µg
EUR 362.5

Antifungal Protein (AFP) Antibody (HRP)

abx333617-20g 20 µg
EUR 162.5

Antifungal Protein (AFP) Antibody (HRP)

abx333617-50g 50 µg
EUR 250

Antifungal Protein (AFP) Antibody (FITC)

20-abx333618
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Antifungal Protein (AFP) Antibody (FITC)

abx333618-100g 100 µg
EUR 362.5

Antifungal Protein (AFP) Antibody (FITC)

abx333618-20g 20 µg
EUR 162.5

Antifungal Protein (AFP) Antibody (FITC)

abx333618-50g 50 µg
EUR 250

Antifungal Protein (AFP) Antibody (Biotin)

20-abx333619
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Antifungal Protein (AFP) Antibody (Biotin)

abx333619-100g 100 µg
EUR 362.5

Antifungal Protein (AFP) Antibody (Biotin)

abx333619-20g 20 µg
EUR 162.5

Antifungal Protein (AFP) Antibody (Biotin)

abx333619-50g 50 µg
EUR 250

Recombinant Diospyros texana Antifungal protein

MBS1134700-005mgBaculovirus 0.05mg(Baculovirus)
EUR 890

Recombinant Diospyros texana Antifungal protein

MBS1134700-005mgEColi 0.05mg(E-Coli)
EUR 510

Recombinant Diospyros texana Antifungal protein

MBS1134700-005mgYeast 0.05mg(Yeast)
EUR 710

Recombinant Diospyros texana Antifungal protein

MBS1134700-02mgEColi 0.2mg(E-Coli)
EUR 670

Recombinant Diospyros texana Antifungal protein

MBS1134700-05mgEColi 0.5mg(E-Coli)
EUR 715

Recombinant Cullen corylifolia Antifungal protein

MBS1242351-005mgBaculovirus 0.05mg(Baculovirus)
EUR 880

Recombinant Cullen corylifolia Antifungal protein

MBS1242351-005mgEColi 0.05mg(E-Coli)
EUR 500

Recombinant Cullen corylifolia Antifungal protein

MBS1242351-005mgYeast 0.05mg(Yeast)
EUR 700

Recombinant Cullen corylifolia Antifungal protein

MBS1242351-02mgEColi 0.2mg(E-Coli)
EUR 655

Recombinant Cullen corylifolia Antifungal protein

MBS1242351-05mgEColi 0.5mg(E-Coli)
EUR 695

Recombinant Hordeum vulgare Antifungal protein S

MBS1014779-002mgBaculovirus 0.02mg(Baculovirus)
EUR 965

Recombinant Hordeum vulgare Antifungal protein S

MBS1014779-002mgEColi 0.02mg(E-Coli)
EUR 520

Recombinant Hordeum vulgare Antifungal protein S

MBS1014779-002mgYeast 0.02mg(Yeast)
EUR 715

Recombinant Hordeum vulgare Antifungal protein S

MBS1014779-01mgEColi 0.1mg(E-Coli)
EUR 615

Recombinant Hordeum vulgare Antifungal protein S

MBS1014779-01mgYeast 0.1mg(Yeast)
EUR 835

Recombinant Musca domestica Antifungal protein 1

MBS1153926-005mgBaculovirus 0.05mg(Baculovirus)
EUR 890

Recombinant Musca domestica Antifungal protein 1

MBS1153926-005mgEColi 0.05mg(E-Coli)
EUR 510

Recombinant Musca domestica Antifungal protein 1

MBS1153926-005mgYeast 0.05mg(Yeast)
EUR 710

Recombinant Musca domestica Antifungal protein 1

MBS1153926-02mgEColi 0.2mg(E-Coli)
EUR 675

Recombinant Musca domestica Antifungal protein 1

MBS1153926-05mgEColi 0.5mg(E-Coli)
EUR 715

Recombinant Hordeum vulgare Antifungal protein R

MBS1218281-002mgBaculovirus 0.02mg(Baculovirus)
EUR 970

Recombinant Hordeum vulgare Antifungal protein R

MBS1218281-002mgEColi 0.02mg(E-Coli)
EUR 530

Recombinant Hordeum vulgare Antifungal protein R

MBS1218281-002mgYeast 0.02mg(Yeast)
EUR 720

Recombinant Hordeum vulgare Antifungal protein R

MBS1218281-01mgEColi 0.1mg(E-Coli)
EUR 625

Recombinant Hordeum vulgare Antifungal protein R

MBS1218281-01mgYeast 0.1mg(Yeast)
EUR 845

Recombinant Malva parviflora Antifungal protein 4

MBS1168035-002mgBaculovirus 0.02mg(Baculovirus)
EUR 965

Recombinant Malva parviflora Antifungal protein 4

MBS1168035-002mgEColi 0.02mg(E-Coli)
EUR 520

Recombinant Malva parviflora Antifungal protein 4

MBS1168035-002mgYeast 0.02mg(Yeast)
EUR 715

Recombinant Malva parviflora Antifungal protein 4

MBS1168035-01mgEColi 0.1mg(E-Coli)
EUR 615

Recombinant Malva parviflora Antifungal protein 4

MBS1168035-01mgYeast 0.1mg(Yeast)
EUR 835

Recombinant Malva parviflora Antifungal protein 3

MBS1180068-005mgBaculovirus 0.05mg(Baculovirus)
EUR 875

Recombinant Malva parviflora Antifungal protein 3

MBS1180068-005mgEColi 0.05mg(E-Coli)
EUR 495

Recombinant Malva parviflora Antifungal protein 3

MBS1180068-005mgYeast 0.05mg(Yeast)
EUR 695

Recombinant Malva parviflora Antifungal protein 3

MBS1180068-02mgEColi 0.2mg(E-Coli)
EUR 645

Recombinant Malva parviflora Antifungal protein 3

MBS1180068-05mgEColi 0.5mg(E-Coli)
EUR 705

Recombinant Passiflora alata Antifungal protein 1

MBS1148141-005mgBaculovirus 0.05mg(Baculovirus)
EUR 890

Recombinant Passiflora alata Antifungal protein 1

MBS1148141-005mgEColi 0.05mg(E-Coli)
EUR 510

Recombinant Passiflora alata Antifungal protein 1

MBS1148141-005mgYeast 0.05mg(Yeast)
EUR 710

Recombinant Passiflora alata Antifungal protein 1

MBS1148141-02mgEColi 0.2mg(E-Coli)
EUR 675

Recombinant Passiflora alata Antifungal protein 1

MBS1148141-05mgEColi 0.5mg(E-Coli)
EUR 715

Recombinant Malva parviflora Antifungal protein 5

MBS1105543-002mgBaculovirus 0.02mg(Baculovirus)
EUR 965

Recombinant Malva parviflora Antifungal protein 5

MBS1105543-002mgEColi 0.02mg(E-Coli)
EUR 520

Recombinant Malva parviflora Antifungal protein 5

MBS1105543-002mgYeast 0.02mg(Yeast)
EUR 720

Recombinant Malva parviflora Antifungal protein 5

MBS1105543-01mgEColi 0.1mg(E-Coli)
EUR 620

Recombinant Malva parviflora Antifungal protein 5

MBS1105543-01mgYeast 0.1mg(Yeast)
EUR 840

Recombinant Arachis hypogaea Antifungal protein 1

MBS1235254-005mgBaculovirus 0.05mg(Baculovirus)
EUR 875

Recombinant Arachis hypogaea Antifungal protein 1

MBS1235254-005mgEColi 0.05mg(E-Coli)
EUR 495

Recombinant Arachis hypogaea Antifungal protein 1

MBS1235254-005mgYeast 0.05mg(Yeast)
EUR 695

Recombinant Arachis hypogaea Antifungal protein 1

MBS1235254-02mgEColi 0.2mg(E-Coli)
EUR 645

Recombinant Arachis hypogaea Antifungal protein 1

MBS1235254-05mgEColi 0.5mg(E-Coli)
EUR 705

Recombinant Solanum tuberosum Antifungal protein J

MBS1165381-005mgBaculovirus 0.05mg(Baculovirus)
EUR 880

Recombinant Solanum tuberosum Antifungal protein J

MBS1165381-005mgEColi 0.05mg(E-Coli)
EUR 500

Recombinant Solanum tuberosum Antifungal protein J

MBS1165381-005mgYeast 0.05mg(Yeast)
EUR 700

Recombinant Solanum tuberosum Antifungal protein J

MBS1165381-02mgEColi 0.2mg(E-Coli)
EUR 655

Recombinant Solanum tuberosum Antifungal protein J

MBS1165381-05mgEColi 0.5mg(E-Coli)
EUR 695

Recombinant Passiflora edulis Antifungal protein 1

MBS1170471-005mgBaculovirus 0.05mg(Baculovirus)
EUR 885

Recombinant Passiflora edulis Antifungal protein 1

MBS1170471-005mgEColi 0.05mg(E-Coli)
EUR 505

Recombinant Passiflora edulis Antifungal protein 1

MBS1170471-005mgYeast 0.05mg(Yeast)
EUR 705

Recombinant Passiflora edulis Antifungal protein 1

MBS1170471-02mgEColi 0.2mg(E-Coli)
EUR 665

Recombinant Passiflora edulis Antifungal protein 1

MBS1170471-05mgEColi 0.5mg(E-Coli)
EUR 705

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