Knockout of the caspase Eight associated protein 2 gene improves recombinantprotein expression in HEK293 cells
September 25, 2020
Dynamics of serological responses to outlined recombinantproteins all through Schistosoma mansoni an an infection in mice sooner than and after the treatment with praziquantel
To do away with schistosomiasis, acceptable diagnostic assessments are required to observe its prevalence and transmission, significantly throughout the settings with low endemicity ensuing from the consecutive mass drug administration. Antibodies that react with each crude soluble schistosome egg antigens or soluble worm antigen preparations have been used to observe an an infection in low-prevalence areas.
Nonetheless, these detection methods can’t discriminate current and former infections and are cross-reactive with completely different parasites because of every antigens embody fairly just a few proteins and glycans from schistosomes, and regular preparations need maintenance of the life cycle of the schistosome.
To guage the potential utility of 9 recombinant Schistosoma mansoni proteins as single outlined antigens for serological evaluation, we monitored the kinetics of antibodies to each antigen all through S. mansoni an an infection in mice sooner than and after the treatment with praziquantel. C57BL/6 mice had been contaminated with 50 cercariae.
The levels of immunoglobulin G (IgG) raised in the direction of 5 recombinant antigens (RP26, sm31, sm32, GST, and LAP1) significantly elevated as early as 2-4 weeks after an an infection and shortly declined by 2 weeks after the treatment, whereas these raised in the direction of crude S. mansoni egg antigens or completely different antigens remained elevated prolonged after the treatment.
The IgG1 raised in the direction of RP26, sm31, and serpin decreased after the treatment with praziquantel, whereas the IgE raised in the direction of serpin declined strikingly after the treatment. This analysis clarifies the dynamics of the serological responses to recombinant S. mansoni proteins all through an an infection and after the treatment with praziquantel and identifies a variety of candidate antigens with potential utility throughout the monitoring and surveillance of schistosomiasis in the direction of the elimination of schistosomiasis.
Description: Quantitativesandwich ELISA kit for measuring Goat Osteocalcin (BGLAP) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Goat Osteocalcin(BGLAP) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Monkey Osteocalcin (BGLAP) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Monkey Osteocalcin(BGLAP) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: PMF1-BGLAP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 234 amino acids (1-211aa) and having a molecular mass of 26.2kDa.
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against BGLAP. Recognizes BGLAP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200
Description: A polyclonal antibody against BGLAP. Recognizes BGLAP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:5000, IHC:1:50-1:200
Description: A polyclonal antibody against BGLAP. Recognizes BGLAP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:5000, IHC:1:50-1:200
Description: A polyclonal antibody against BGLAP. Recognizes BGLAP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200
Description: A polyclonal antibody against BGLAP. Recognizes BGLAP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:5000, IHC:1:50-1:200
Description: Description of target: highly conserved protein associated with mineralized bone matrix; protein is secreted by calcified tissues and is regulated by vitamin D3 [RGD, Feb 2006];Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 6.3 pg/mL
Description: Description of target: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. ;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.078 pg/mL
Description: Description of target: highly conserved protein associated with mineralized bone matrix; protein is secreted by calcified tissues and is regulated by vitamin D3.;Species reactivity: Rat;Application: ELISA;Assay info: ;Sensitivity: < 6.3pg/mL
Description: Description of target: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 31.31 pg/mL
Description: A competitive ELISA for quantitative measurement of Rat Osteocalcin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Osteocalcin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Osteocalcin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product.
Description: This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product.
Description: This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product.
Description: This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product.
Should the Rat Osteocalcin (OC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Osteocalcin (OC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.
Should the Rat Osteocalcin (OC) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Osteocalcin (OC) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.
Description: A competitive ELISA for quantitative measurement of Rat Undercarboxylated Osteocalcin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Undercarboxylated Osteocalcin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Undercarboxylated Osteocalcin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Carboxylated Osteocalcin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Carboxylated Osteocalcin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Carboxylated Osteocalcin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Osteocalcin (OC) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Osteocalcin (OC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Osteocalcin (OC) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Osteocalcin (OC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Osteocalcin (OC) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Osteocalcin (OC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Osteocalcin (OC) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Osteocalcin (OC) in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Known also as Osteocalcin elisa. Alternative names of the recognized antigen: BGLAP
OT
BGP
Bone Gla Protein
Bone Gamma-Carboxyglutamate Protein
Gamma-carboxyglutamic acid-containing protein
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Osteocalcin (OC) in samples from serum, plasma, tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Knockout of the caspase Eight associated protein 2 gene improves recombinantprotein expression in HEK293 cells by the use of up-regulation of the cyclin-dependent kinase inhibitor 2A gene
Cell strains utilized in bioproduction are routinely engineered to boost their manufacturing effectivity. Fairly just a few strategies, corresponding to random mutagenesis, RNA interference screens, and transcriptome analyses have been employed to ascertain environment friendly engineering targets.
A genome-wide siRNA show display screen beforehand acknowledged the CASP8AP2 gene as a potential engineering aim for improved expression of recombinant protein throughout the HEK293 cell line. Proper right here, we validate the CASP8AP2 gene as an engineering aim in HEK293 cells by knocking it out using CRISPR/Cas9 genome modifying and assessing the influence of its knockout on recombinant protein expression, cell growth, cell viability, and whole gene expression. HEK293 cells lacking CASP8AP2 confirmed a 7-fold enhance particularly expression of recombinant luciferase and a 2.5-fold enhance particularly expression of recombinant SEAP, with out significantly affecting cell progress and viability.
Transcriptome analysis revealed that de-regulation of the cell cycle, significantly the upregulation of the cyclin dependent kinase inhibitor 2A (CDKN2A) gene, contributed to the advance in recombinant protein expression in CASP8AP2 poor cells. The outcomes validate the CASP8AP2 gene is a viable engineering aim for improved recombinant protein expression throughout the HEK293 cell line. This textual content is protected by copyright. All rights reserved.
Enchancment of a constitutive and an auto-inducible high-yield expression system for recombinantprotein manufacturing throughout the microalga Nannochloropsis oceanica
Photoautotrophic microalgae provide an superior potential as novel hosts for atmosphere pleasant recombinant protein manufacturing. Nannochloropsis oceanica produces an awfully extreme content material materials of polyunsaturated fatty acids, and its sturdy progress traits, revealed genome sequence and atmosphere pleasant nuclear transformation make N. oceanica a promising candidate for biotechnological functions.
To find out a sturdy and versatile system for recombinant protein manufacturing, we cloned six endogenous, doubtlessly constitutive or inducible promoters from N. oceanica stress CCMP1779 and investigated their vitality using monomeric Venus as reporter gene.
Microscopic pre-screening of explicit particular person transformants revealed that the promoters of elongation problem (EF), tubulin (TUB) and nitrate reductase (NR) enabled extreme reporter gene expression. Comparative quantitative analyses of transformant populations by stream cytometry and qRT-PCR demonstrated the perfect Venus expression from the EF promoter and the NR promoter if extended by an N-terminal 14-amino acid chief sequence.
The kinetics of reporter gene expression had been analysed all through photobioreactor cultivation, attaining Venus yields of 0.3% (for EF) and 4.9% (for NR::LS) of full soluble protein. Since inducible expression applications permit the manufacturing of toxic proteins, we developed an auto-induction medium for the NR promoter transformants.
By switching the N provide from ammonium to nitrate in the presence of low ammonium concentrations, the place to start of Venus induction may probably be fine-tuned and shifted within the course of exponential progress half whereas sustaining extreme recombinant protein yields.
Taken collectively, we reveal {{that a}} model recombinant protein could possibly be produced robustly and at very extreme ranges in N. oceanica not solely beneath constitutive however as well as beneath auto-inducible cultivation conditions.
KEY POINTS:
• Nannochloropsis oceanica might operate host for recombinant protein manufacturing.
• Comparative promoter vitality analyses had been carried out for twelve completely completely different constructs.
• Sturdy high-yield recombinant protein manufacturing was achieved beneath constitutive conditions.
• The nitrate reductase promoter enabled protein manufacturing beneath auto-induction conditions.
Should the Human Apolipoprotein E (APOE) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Apolipoprotein E (APOE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Human Apolipoprotein E (APOE) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Apolipoprotein E (APOE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Mouse Apolipoprotein E (APOE) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Apolipoprotein E (APOE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Mouse Apolipoprotein E (APOE) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Apolipoprotein E (APOE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Porcine Apolipoprotein E (APOE) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Apolipoprotein E (APOE) in samples from serum, plasma or other biological fluids.
Should the Porcine Apolipoprotein E (APOE) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Apolipoprotein E (APOE) in samples from serum, plasma or other biological fluids.
Should the Rat Apolipoprotein E (APOE) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Apolipoprotein E (APOE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Rat Apolipoprotein E (APOE) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Apolipoprotein E (APOE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Gentaur's ApoE ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit ApoE. Standards or samples are added to the micro ELISA plate wells and combined with t
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rabbit Apolipoprotein E (APOE) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit Apolipoprotein E (APOE) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rabbit Apolipoprotein E (APOE) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit Apolipoprotein E (APOE) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rabbit Apolipoprotein E (APOE) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit Apolipoprotein E (APOE) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rabbit Apolipoprotein E (APOE) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit Apolipoprotein E (APOE) in serum, plasma and other biological fluids.
Known also as Apolipoprotein E elisa. Alternative names of the recognized antigen: Apo-E
AD2
Apoprotein
Alzheimer Disease 2(E4-Associated, Late Onset
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Rabbit Apolipoprotein E (APOE) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.
Gentaur's ApoE CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoE . Standards or samples are added to the micro CLIA plate wells and combined with the sp
Gentaur's ApoE ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoE. Standards or samples are added to the micro ELISA plate wells and combined with th
Gentaur's ApoE CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ApoE . Standards or samples are added to the micro CLIA plate wells and combined with the
Gentaur's ApoE ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ApoE. Standards or samples are added to the micro ELISA plate wells and combined with th
Gentaur's ApoE ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoE. Standards or samples are added to the micro ELISA plate wells and combined with the
A monoclonal antibody specific to Apolipoprotein E (APOE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apolipoprotein E (APOE) and unlabeled Apolipoprotein E (APOE) (Standards or samples)
Description: A competitive Inhibition ELISA kit for detection of Apolipoprotein E from Rabbit in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
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