Immunization with a RecombinantProtein of Trichinella britovi 14-3-Three Triggers an Immune Response nevertheless
September 25, 2020
Establishment of a two-step purification scheme for tag-free recombinant Taiwan native norovirus P and VP1 proteins
The protruding (P) space of the important thing capsid protein VP1 of norovirus (NoV) is the important issue for immune recognition and host receptor binding. The heterologous P protein expressed by Pichia pastoris self-assembles into P particles. Nonetheless, tag-free NoV protein purification schemes have hardly been reported because of low isoelectric degree of NoV proteins, which ends up in extraordinarily aggressive binding between the aim protein and yeast host cell proteins at alkaline pH.
On this analysis, a two-step purification scheme based totally on flooring histidines and the fee on the NoV GII.4 stress P protein was developed. Using HisTrap and ion commerce chromatography, the P protein was straight purified, with a restoration of 28.1% and purity of 82.1%. Equally, the NoV capsid protein VP1 was moreover purified using HisTrap and gel filtration chromatography based totally on native flooring histidines and self-assembly potential, with 20% restoration and over 90% purity.
Dynamic delicate scattering and transmission electron microscopy analyses of the purified NoV P revealed that most of these small P particles had been triangle-, square- and ring-shaped, with a diameter of roughly 14 nm, and that the purified NoV VP1 self-assembles into particles with a diameter of roughly 47 nm. Every the purified NoV P and VP1 particles retained human histo-blood group antigen-binding potential, as evidenced by a saliva-binding assay.
Description: Quantitativesandwich ELISA kit for measuring Human glypican 6 (GPC6) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human glypican 6 (GPC6) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Myostatin is a TGF-β family member that acts as an inhibitor of skeletal muscle growth. This muscle-specific cytokine interacts with Activin type I and type II receptors, and suppresses myoblast proliferation by arresting cell-cycle in the G1 phase. Suppression of myostatin activity facilitates muscle formation and may be useful in reducing and/or preventing adiposity and type-2 diabetes. Myostatin activity can be blocked by the Activin-binding protein Follistatin, and by the propeptide of Myostatin. Recombinant Human myostatin is a 25.0 kDa protein consisting of two identical 109 amino acid polypeptides linked by a single disulfide bond.
Description: HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues.*Manufactured using (BTI-Tn-5B1-4) cells under license from Boyce Thompson Institute for Plant Research, Inc.
Description: PlGF-3 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PlGF-3 is expressed exclusively in the placenta. It signals through the VEGFR-1/FLT1 receptor and stimulates endothelial cell proliferation and migration. PlGF-3 lacks heparin binding affinity. Recombinant human PlGF-3 is a 45.7 kDa disulfide-linked homodimeric protein of two 203 amino acid polypeptide chains.
Description: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.
Description: IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. Recombinant human IL-7 is a 17.4 kDa protein containing 153 amino acid residues.
BCL2 Human, B-Cell Lymphoma Protein 2 Alpha Human Recombinant Protein, His Tag
Description: BCL2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing amino acids 1-211 and having a molecular mass of 25.4 kDa. The BCL2 is fused to a 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Description: IL-1 beta (IL-1β) is a member of the interleukin 1 family of cytokines. The IL-1 beta cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. Human IL-1 beta Recombinant Protein is purified interleukin-1 beta cytokine produced in yeast.
VEGF Human, Vascular Endothelial Growth Factor Human Recombinant Protein, His
Description: Vascular Endothelial Growth Factor Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain (aa 207-371) containing a total of 185 amino acids and having a molecular mass of 21.3 kDa (corresponding to Isoform L-VEGF165 UniProt acc#P15692-11). ;The VEGF is fused to a 20 a.a His-tag at N-terminus and is purified by proprietary chromatographic techniques.
Description: SDF-1α and β are stromal derived CXC chemokines, and signal through the CXCR4 receptor. SDF-1α and β chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV suppressive activity in cells expressing the CXCR4 receptor. Recombinant human SDF-1β is an 8.5 kDa protein containing 72 amino acid residues.
Description: FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues.
Description: Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi's sarcoma cells, OSH can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi's sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptor in hepatoma cells. OSM share several structural and functional characteristics with LIF, IL-6, and CNTF. Human OSM is active on murine cells. Recombinant human Oncostatin M is a 25.7 kDa protein, containing 227 amino acid residues.
Description: The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. The physiological role of TGF-β3 is still unknown but its expression pattern suggests a role in the regulation of certain development processes. Recombinant human TGF-β3 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.
SNCA Alpha Synuclein 96-140 Human Recombinant Protein
Description: A-Synuclein 96-140 Human Recombinant which is a deletion mutant of the a-synuclein amino acids 96-140, produced in E.Coli is a single, non-glycosylated polypeptide chain of 46 amino acids having a molecular mass of 5.2kDa, with an additional Met attached at the N-terminus. The Recombinant Human a-Synuclein 96-140 is purified by proprietary chromatographic techniques.
Description: GPC3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 544 amino acids (25-559a.a.) and having a molecular mass of 61.8kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). GPC3 is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Description: GPC4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids (401-529a.a) and having a molecular mass of 18kDa.GPC4 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
FLT1 Human, Vascular Endothelial Growth Factor receptor-1 Human Recombinant Protein, His Tag
Description: FLT1 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 298 amino acids fragment (31-328) corresponding to the IgG like domains 1-3 from the mature soluble FLT1 protein, having a total molecular mass of 43kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. ;The FLT1 His is purified by proprietary chromatographic techniques.
Recombinant Human TGF-Beta 1 (HEK 293 derived) Protein
Description: The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Human TGF-β1 is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond.
Description: SPA-Cys long Recombinant produced in E.Coli is a single non-glycosylated polypeptide chain with a Cys on C-terminus. SPA-Cys is comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 423 amino acids and having a molecular mass of 46.7kDa containing little or no carbohydrate.
Description: CXCL9 (MIG) is a T-cell chemoattractant. Induced by IFN-gamma (IFN-γ), the ELR-negative chemokine CXCL9 (MIG) elicits its effects by binding to the cell surface chemokine receptor CXCR3. Swine CXCL9 Recombinant Protein is purified CXCL9 (MIG) produced in yeast.
Description: The ELR-negative CXC chemokine CXCL10 (IP-10) has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. Ovine CXCL10 Recombinant Protein is purified CXCL10 (IP-10) produced in yeast.
Description: The small chemotactic cytokine CCL4 (MIP-1 beta) is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is involved in several inflammatory and autoimmune diseases including viral infection such as HIV-1/AIDS. Rat CCL4 Recombinant Protein is purified chemokine ligand 4 (CCL4, MIP-1 beta) produced in yeast.
Description: A competitive ELISA for quantitative measurement of Human Glypican 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Glypican 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Glypican 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Glypican 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Glypican 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Glypican 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Glypican 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Glypican 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Glypican 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Glypican 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Glypican 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Glypican 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. GM-CSF stimulates stem cells to produce granulocytes and monocytes and is glycosylated in its mature form.
Immunization with a RecombinantProtein of Trichinella britovi 14-3-Three Triggers an Immune Response nevertheless No Security in Mice
14-3-Three proteins are present in all eukaryotic organisms and are ubiquitously expressed in a broad fluctuate of tissues and cellular compartments. They’re regulatory adapter proteins that play key roles in a variety of signaling pathways, and have been proposed as applicable targets for the administration and detection of certain parasites.
Trichinella britovi is a widely-distributed parasitic nematode, transmitted by the use of ingestion of meat merchandise containing invasive larvae. The present analysis describes the cloning and expression of Tb14-3-3, and investigates the immunological and defending potential of the recombinant protein. Immunization of mice with rTb14-3-Three triggered an IgG response, and essential variations, throughout the profiles of secreted cytokines observed in vitro, between experimental groups.
Nonetheless, neither explicit antibodies, nor elevated secretion of IFNγ, IL-4, and IL-10 cytokines, conferred increased security in the direction of an an infection. No low cost in larval burden was observed all through restoration at 48 dpi. Furthermore, rTb14-3-Three was not acknowledged by sera from the contaminated administration mice, other than one, suggesting some mismatch between native and recombinant Tb14-3-Three antigenic web sites. Attributable to this reality, sooner than 14-3-Three could possibly be considered a potential software program for Trichinella detection and vaccination, further evaluation regarding its aim proteins, and exact explicit carry out, is required.
Inhibition of E. coli host RNA Polymerase Permits Setting pleasant Extracellular RecombinantProtein Manufacturing by Enhancing Outer Membrane Leakiness
Recombinant proteins in Escherichia coli are usually expressed contained within the cell. With the rising curiosity in regular cultivation, secretion of product to the medium is simply not solely a revenue nevertheless a necessity in future bioprocessing. On this analysis, we current that induced decoupling of progress and heterologous gene expression throughout the E. coli X-press stress, a stress derived from BL21(DE3), facilitates extracellular recombinant protein manufacturing. We investigated the influence of the strategy parameters temperature and explicit glucose consumption cost (qS ) on progress, productiveness, lysis and leakiness, to go looking out the parameter space allowing extracellular protein manufacturing.
Two model proteins had been used, Protein A and a VHH single-domain antibody, and effectivity was compared with the industrial regular stress BL21(DE3). We current that inducible progress repression throughout the X-press stress vastly mitigates the influence of metabolic burden beneath completely completely different course of conditions.
Furthermore, temperature and qS had been used to control productiveness and leakiness. Inside the X-press stress, extracellular Protein A and VHH titer reached as a lot as 349 mg/g and 19.6 mg/g, respectively, comprising as a lot as 90% of the total soluble product, whereas preserving cell lysis at a minimal. Our findings reveal that the X-press stress constitutes a helpful host for extracellular manufacturing of recombinant proteinwith E. coli. This textual content is protected by copyright. All rights reserved.
SV40 large T-antigen (Neo), EF1a lentiviral particles
Description: Large T antigen - rhesus polyomavirus 560-568 has a peptide sequence of Ser-Glu-Phe-Leu-Leu-Glu-Lys-Arg-Ile.T antigen is required for viral DNA replication, transcription, and virion assembly.
Description: Large T antigen - rhesus polyomavirus 560-568 has a peptide sequence of Ser-Glu-Phe-Leu-Leu-Glu-Lys-Arg-Ile.T antigen is required for viral DNA replication, transcription, and virion assembly.
Description: Large T antigen - rhesus polyomavirus 560-568 has a peptide sequence of Ser-Glu-Phe-Leu-Leu-Glu-Lys-Arg-Ile.T antigen is required for viral DNA replication, transcription, and virion assembly.
Description: Large T antigen - rhesus polyomavirus 560-568 has a peptide sequence of Ser-Glu-Phe-Leu-Leu-Glu-Lys-Arg-Ile.T antigen is required for viral DNA replication, transcription, and virion assembly.
Should the Testosterone (T) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Testosterone (T) in samples from serum, plasma, urine or other biological fluids.
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SV40 large T-antigen (Neo), EF1a lentiviral particles , EF1a lentiviral particles)
SV40 large T-antigen (Puro), EF1a lentiviral particles , CMV lentiviral particles)
SV40 large T-antigen (GFP-Bsd), CMV lentiviral particles , CMV lentiviral particles)
SV40 large T-antigen (GFP-Puro), CMV lentiviral particles , CMV lentiviral particles)
SV40 large T-antigen (RFP-Bsd), CMV lentiviral particles , CMV lentiviral particles)
SV40 large T-antigen (RFP-Puro), CMV lentiviral particles , EF1a lentiviral particles)
SV40 large T-antigen (GFP-Puro), EF1a lentiviral particles , EF1a lentiviral particles)
SV40 large T-antigen (RFP-Bsd), EF1a lentiviral particles , EF1a lentiviral particles)
SV40 large T-antigen (RFP-Puro), EF1a lentiviral particles 
SV40 T Antigen antibody  in PBS, CMV lentiviral particles)
SV40 large T-antigen (Bsd) in PBS, CMV lentiviral particles  in PBS, CMV lentiviral particles)
SV40 large T-antigen (Neo) in PBS, CMV lentiviral particles  in PBS, CMV lentiviral particles)
SV40 large T-antigen (Puro) in PBS, CMV lentiviral particles  in PBS, EF1a lentiviral particles)
SV40 large T-antigen (Bsd) in PBS, EF1a lentiviral particles  in PBS, EF1a lentiviral particles)
SV40 large T-antigen (Neo) in PBS, EF1a lentiviral particles  in PBS, EF1a lentiviral particles)
SV40 large T-antigen (Puro) in PBS, EF1a lentiviral particles  in PBS, CMV lentiviral particles)
SV40 large T-antigen (GFP-Bsd) in PBS, CMV lentiviral particles  in PBS, CMV lentiviral particles)
SV40 large T-antigen (GFP-Puro) in PBS, CMV lentiviral particles  in PBS, CMV lentiviral particles)
SV40 large T-antigen (RFP-Bsd) in PBS, CMV lentiviral particles  in PBS, CMV lentiviral particles)
SV40 large T-antigen (RFP-Puro) in PBS, CMV lentiviral particles  in PBS, EF1a lentiviral particles)
SV40 large T-antigen (GFP-Puro) in PBS, EF1a lentiviral particles  in PBS, EF1a lentiviral particles)
SV40 large T-antigen (RFP-Bsd) in PBS, EF1a lentiviral particles  in PBS, EF1a lentiviral particles)
SV40 large T-antigen (RFP-Puro) in PBS, EF1a lentiviral particles  Protein)
Large T Antigen (LT) Protein  Antibody)
Large T Antigen (LT) Antibody 
Large T antigen - rhesus polyomavirus 560-568 
Immortalized Hamster Hepatocytes-SV40 T+t 
Immortalized Monkey Hepatocytes-SV40 T+t 
pSG5 Large T Plasmid 
T, Pallidum recombinant antigen p15 
T, Pallidum recombinant antigen p17 
T, Pallidum recombinant antigen p45 
T, pallidum recombinant antigen TmpA 
T. Pallidum Antigen 
T-Pro Stacking Buffer 
T-Pro MycoClean spray 
SV40 NLS 
Recombinant human T-lymphocyte activation antigen CD80 
T. pallidum Tp0453 Antigen 
T-Pro EZ Gel Solution 8% 
T-Pro EZ Gel Solution 10% 
T-Pro EZ Gel Solution 12% 
T-Pro EZ Gel Solution 15% 
T-Pro Separating or Resolving Buffer 
T-Pro BCA Protein Assay kit )
T-Pro Transfer Blotting buffer (10X) 
T-Pro Western Blot Stripping Reagent )
T-Pro Aqua EZ Clean (M) )
T-Pro Aqua EZ Clean (S) 
T-Pro EZ stain Gel solution 
T-Pro P-Fect Transfection Reagent )
T-Pro Plasmid Mini Kit (100) )
T-Pro Plasmid Mini Kit (250) )
T-Pro Plasmid Midi Kit (20) )
T-Pro Plasmid Maxi Kit (10) 
Lenti-SV40 Lentivirus 
Adeno-SV40 Adenovirus 
SV40 NLS Peptide 
pGL4.13[luc2- SV40] 
pRL- SV40 Plasmid 
Recombinant Human Cytotoxic T-Lymphocyte Associated Antigen-4 )
Recombinant Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) 
T-Pro BCA Protein Assay Reagent A 
T-Pro BCA Protein Assay Reagent B )
T-Pro Bradford Protein Assay kit(1X) )
T-Pro Fast Blocking Buffer (in PBS) )
T-Pro Fast Blocking Buffer (in TBS) )
T-Pro Genomic DNA Midi Kit (20) )
T-Pro Genomic DNA Mini Kit (100)  ELISA Kit)
Testosterone (T) ELISA Kit