Immunization with a RecombinantProtein of Trichinella britovi 14-3-Three Triggers an Immune Response nevertheless
Establishment of a two-step purification scheme for tag-free recombinant Taiwan native norovirus P and VP1 proteins
The protruding (P) space of the important thing capsid protein VP1 of norovirus (NoV) is the important issue for immune recognition and host receptor binding. The heterologous P protein expressed by Pichia pastoris self-assembles into P particles. Nonetheless, tag-free NoV protein purification schemes have hardly been reported because of low isoelectric degree of NoV proteins, which ends up in extraordinarily aggressive binding between the aim protein and yeast host cell proteins at alkaline pH.
On this analysis, a two-step purification scheme based totally on flooring histidines and the fee on the NoV GII.4 stress P protein was developed. Using HisTrap and ion commerce chromatography, the P protein was straight purified, with a restoration of 28.1% and purity of 82.1%. Equally, the NoV capsid protein VP1 was moreover purified using HisTrap and gel filtration chromatography based totally on native flooring histidines and self-assembly potential, with 20% restoration and over 90% purity.
Dynamic delicate scattering and transmission electron microscopy analyses of the purified NoV P revealed that most of these small P particles had been triangle-, square- and ring-shaped, with a diameter of roughly 14 nm, and that the purified NoV VP1 self-assembles into particles with a diameter of roughly 47 nm. Every the purified NoV P and VP1 particles retained human histo-blood group antigen-binding potential, as evidenced by a saliva-binding assay.
Human Glypican-1 (GPC1) Protein |
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abx060282-50g | Abbexa | 50 µg | Ask for price |
Human Glypican-1 (GPC1) Protein |
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abx694143-10nmol | Abbexa | 10 nmol | EUR 400 |
Human Glypican-1 (GPC1) Protein |
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abx694143-5nmol | Abbexa | 5 nmol | EUR 300 |
Human Glypican-6 (GPC6) |
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1-CSB-YP009708HU | Cusabio |
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Description: Recombinant Human Glypican-6(GPC6) expressed in Yeast |
Human Glypican 1 (GPC1) Protein |
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20-abx066889 | Abbexa |
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Human Glypican 3 (GPC3) Protein |
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20-abx066892 | Abbexa |
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Human Glypican 4 (GPC4) Protein |
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20-abx066893 | Abbexa |
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Human Glypican 3 (GPC3) Protein |
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abx060155-100ug | Abbexa | 100 ug | EUR 1362 |
Human Glypican 3 (GPC3) Protein |
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abx060263-1mg | Abbexa | 1 mg | EUR 2532 |
Human Glypican 2 (GPC2) Protein |
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20-abx653616 | Abbexa |
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Human Glypican 3 (GPC3) Protein |
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abx060155-100g | Abbexa | 100 µg | Ask for price |
Human Glypican 3 (GPC3) Protein |
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abx060155-10g | Abbexa | 10 µg | EUR 1037.5 |
Human Glypican 3 (GPC3) Protein |
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abx060155-50g | Abbexa | 50 µg | EUR 2987.5 |
Human Glypican 3 (GPC3) Protein |
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abx060263-100g | Abbexa | 100 µg | Ask for price |
Human Glypican 3 (GPC3) Protein |
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abx060263-10g | Abbexa | 10 µg | EUR 1975 |
Human Glypican 3 (GPC3) Protein |
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abx060263-50g | Abbexa | 50 µg | Ask for price |
Human Glypican 1 (GPC1) Protein |
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abx066889-100g | Abbexa | 100 µg | EUR 425 |
Human Glypican 1 (GPC1) Protein |
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abx066889-10g | Abbexa | 10 µg | EUR 187.5 |
Human Glypican 1 (GPC1) Protein |
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abx066889-50g | Abbexa | 50 µg | EUR 300 |
Human Glypican 3 (GPC3) Protein |
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abx066892-100g | Abbexa | 100 µg | EUR 512.5 |
Human Glypican 3 (GPC3) Protein |
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abx066892-10g | Abbexa | 10 µg | EUR 212.5 |
Human Glypican 3 (GPC3) Protein |
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abx066892-50g | Abbexa | 50 µg | EUR 362.5 |
Human Glypican 4 (GPC4) Protein |
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abx066893-100g | Abbexa | 100 µg | EUR 625 |
Human Glypican 4 (GPC4) Protein |
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abx066893-10g | Abbexa | 10 µg | EUR 237.5 |
Human Glypican 4 (GPC4) Protein |
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abx066893-50g | Abbexa | 50 µg | EUR 425 |
Human Glypican 2 (GPC2) Protein |
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abx653616-100g | Abbexa | 100 µg | EUR 1800 |
Human Glypican 3 (GPC3) Protein |
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abx680130-10g | Abbexa | 10 µg | EUR 225 |
Human Glypican 3 (GPC3) Protein |
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abx680130-50g | Abbexa | 50 µg | EUR 325 |
Human Glypican 5 (GPC5) Protein |
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abx691793-100g | Abbexa | 100 µg | EUR 837.5 |
Human Glypican 5 (GPC5) Protein |
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abx691793-1mg | Abbexa | 1 mg | Ask for price |
Human Glypican 3 Protein (Gln 25-His 559) |
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VAng-1696Lsx-100g | Creative Biolabs | 100 µg | EUR 1116 |
Description: Human Glypican 3 (GPC3) is expressed in human 293 cells. (Uniprot ID: P51654-1) |
Human Glypican 3 Protein (Gln 25-His 559) |
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VAng-1696Lsx-1mg | Creative Biolabs | 1 mg | EUR 5998.8 |
Description: Human Glypican 3 (GPC3) is expressed in human 293 cells. (Uniprot ID: P51654-1) |
Recombinant Human Glypican 3 Protein, His Tag |
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E40KMP1201 | EnoGene | 20ug | EUR 495 |
Recombinant Human Glypican 1 Protein, His Tag |
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E40KMP1589 | EnoGene | 20ug | EUR 495 |
Human Glypican 2 Protein (Ser 24-Gly 554) [Fc] |
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VAng-1694Lsx-100g | Creative Biolabs | 100 µg | EUR 1215.6 |
Description: Human Glypican 2 (GPC2), Fc Tag, is expressed in human 293 cells. (Uniprot ID: Q8N158-1) |
Human Glypican 2 Protein (Ser 24-Gly 554) [Fc] |
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VAng-1694Lsx-1mg | Creative Biolabs | 1 mg | EUR 7682.4 |
Description: Human Glypican 2 (GPC2), Fc Tag, is expressed in human 293 cells. (Uniprot ID: Q8N158-1) |
Human Glypican-6,GPC-6 ELISA Kit |
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YLA3605HU-48T | Shanghai YL Biotech | 48T | Ask for price |
Human Glypican-6,GPC-6 ELISA Kit |
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YLA3605HU-96T | Shanghai YL Biotech | 96T | Ask for price |
Human Glypican 2 Protein (Ser 24-Gly 554) [His] |
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VAng-1695Lsx-100g | Creative Biolabs | 100 µg | EUR 1215.6 |
Description: Human Glypican 2 (GPC2), His Tag, is expressed in human 293 cells. (Uniprot ID: Q8N158-1) |
Human Glypican 2 Protein (Ser 24-Gly 554) [His] |
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VAng-1695Lsx-1mg | Creative Biolabs | 1 mg | EUR 7682.4 |
Description: Human Glypican 2 (GPC2), His Tag, is expressed in human 293 cells. (Uniprot ID: Q8N158-1) |
Human Glypican 1 Protein (Asp 24-Ser 530) [Fc] |
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VAng-1692Lsx-100g | Creative Biolabs | 100 µg | EUR 1215.6 |
Description: Human Glypican 1 (GPC1), Fc Tag, is expressed in human 293 cells. (Uniprot ID: P35052-1) |
Human Glypican 1 Protein (Asp 24-Ser 530) [Fc] |
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VAng-1692Lsx-1mg | Creative Biolabs | 1 mg | EUR 7682.4 |
Description: Human Glypican 1 (GPC1), Fc Tag, is expressed in human 293 cells. (Uniprot ID: P35052-1) |
Human Glypican 3 Protein (Gln 25-His 559) [Fc] |
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VAng-1697Lsx-100g | Creative Biolabs | 100 µg | EUR 1050 |
Description: Human Glypican 3 (GPC3), Fc tag, is expressed in human 293 cells. (Uniprot ID: NP_004475) |
Human Glypican 3 Protein (Gln 25-His 559) [Fc] |
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VAng-1697Lsx-1mg | Creative Biolabs | 1 mg | EUR 6592.8 |
Description: Human Glypican 3 (GPC3), Fc tag, is expressed in human 293 cells. (Uniprot ID: NP_004475) |
Human Glypican 1 Protein (Asp 24-Ser 530) [His] |
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VAng-1693Lsx-100g | Creative Biolabs | 100 µg | EUR 1215.6 |
Description: Human Glypican 1 (GPC1), His Tag, is expressed in human 293 cells. (Uniprot ID: P35052-1) |
Human Glypican 1 Protein (Asp 24-Ser 530) [His] |
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VAng-1693Lsx-1mg | Creative Biolabs | 1 mg | EUR 7682.4 |
Description: Human Glypican 1 (GPC1), His Tag, is expressed in human 293 cells. (Uniprot ID: P35052-1) |
Human Glypican 3 Protein (Gln 25-His 559) [His] |
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VAng-1699Lsx-100g | Creative Biolabs | 100 µg | EUR 1116 |
Description: Human Glypican 3 (GPC3), His tag, is expressed in human 293 cells. (Uniprot ID: P51654) |
Human Glypican 3 Protein (Gln 25-His 559) [His] |
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VAng-1699Lsx-1mg | Creative Biolabs | 1 mg | EUR 5998.8 |
Description: Human Glypican 3 (GPC3), His tag, is expressed in human 293 cells. (Uniprot ID: P51654) |
Human Glypican 6 ELISA kit |
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E01A4951 | BlueGene | 96T | EUR 700 |
Description: ELISA |
Human Glypican 1 / GPC1 Protein, Fc Tag |
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GP1-H5254 | ACROBIOSYSTEMS | 100ug | EUR 2749.9 |
Description: Human Glypican 1, Fc Tag (GP1-H5254) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 530 (Accession # P35052-1). |
GPC4 Glypican-4 Human Recombinant Protein |
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PROTO75487 | BosterBio | Regular: 10ug | EUR 380.4 |
Description: GPC4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids (401-529a.a) and having a molecular mass of 18kDa.GPC4 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
GPC3 Glypican-3 Human Recombinant Protein |
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PROTP51654 | BosterBio | Regular: 10ug | EUR 380.4 |
Description: GPC3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 544 amino acids (25-559a.a.) and having a molecular mass of 61.8kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). GPC3 is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques. |
Recombinant Human Glypican-6 (GPC6) |
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CSB-YP009708HU | Cusabio | 5689 mg | Ask for price |
Recombinant Human Glypican-6(GPC6) |
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AP72579 | SAB | 1mg | EUR 2303 |
Recombinant Human Glypican-6 (GPC6) |
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CSB-EP009708HU | Cusabio | 1297 mg | Ask for price |
Human Glypican 1 / GPC1 Protein, His Tag |
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GP1-H52H9 | ACROBIOSYSTEMS | 100ug | EUR 2749.9 |
Description: Human Glypican 1, His Tag (GP1-H52H9) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 530 (Accession # P35052-1). |
Human Glypican 2 / GPC2 Protein, His Tag |
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GP2-H52H3 | ACROBIOSYSTEMS | 100ug | EUR 2749.9 |
Description: Human Glypican 2, His Tag (GP2-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gly 554 (Accession # Q8N158-1). |
Human Glypican-6, GPC-6 GENLISA ELISA |
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KBH3115 | Krishgen | 1 x 96 wells | EUR 286 |
Human Glypican-6 (GPC6) ELISA Kit |
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abx525923-96tests | Abbexa | 96 tests | EUR 687.5 |
Human Glypican 3 Protein (Gln 25-His 559) [His] [Avi] |
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VAng-1701Lsx-200g | Creative Biolabs | 200 µg | EUR 4612.8 |
Description: Biotinylated Human Glypican 3 (GPC3), His tag and Avi tag, is expressed in human 293 cells. (Uniprot ID: P51654-1) |
Human Glypican 3 Protein (Gln 25-His 559) [His] [Avi] |
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VAng-1701Lsx-25g | Creative Biolabs | 25 µg | EUR 1215.6 |
Description: Biotinylated Human Glypican 3 (GPC3), His tag and Avi tag, is expressed in human 293 cells. (Uniprot ID: P51654-1) |
Active Recombinant Human Glypican-3/GPC3 Protein |
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RP00138 | Abclonal | 10 μg | EUR 110.5 |
Active Recombinant Human Glypican-1/GPC1 Protein |
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RP00297 | Abclonal | 20 μg | EUR 175.5 |
Glypican-4, 511 a.a. Protein |
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20-abx262795 | Abbexa |
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Glypican-4, 511 a.a. Protein |
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abx262795-10mg | Abbexa | 10 mg | EUR 325 |
Glypican-4, 511 a.a. Protein |
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abx262795-25mg | Abbexa | 25 mg | EUR 6575 |
Glypican-4, 511 a.a. Protein |
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abx262795-5mg | Abbexa | 5 mg | EUR 225 |
Human glypican 6 (GPC6) ELISA kit |
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EKC33881-48T | Biomatik Corporation | 48T | EUR 535.99 |
Human glypican 6 (GPC6) ELISA kit |
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EKC33881-5x96T | Biomatik Corporation | 5x96T | EUR 3637.08 |
Human glypican 6 (GPC6) ELISA kit |
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EKC33881-96T | Biomatik Corporation | 96T | EUR 765.7 |
Human glypican 6(GPC6) Elisa Kit |
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EK712547 | AFG Bioscience LLC | 96 Wells | EUR 0.27 |
Human Glypican 6 (GPC6) ELISA Kit |
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AE41487HU-48Tests | Abebio | 48 Tests | EUR 325 |
Description: Human (Homo sapiens) |
Human Glypican 6 (GPC6) ELISA Kit |
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AE41487HU-96Tests | Abebio | 96 Tests | EUR 610 |
Description: Human (Homo sapiens) |
Human glypican 6 (GPC6) ELISA kit |
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CSB-EL009708HU-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
Description: Quantitativesandwich ELISA kit for measuring Human glypican 6 (GPC6) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human glypican 6 (GPC6) ELISA kit |
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1-CSB-EL009708HU | Cusabio |
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Description: Quantitativesandwich ELISA kit for measuring Human glypican 6 (GPC6) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Human Glypican 6(GPC6)ELISA Kit |
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QY-E02274 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Glypican 3 / GPC3 (359-559) Protein, His Tag |
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GP3-H52H5 | ACROBIOSYSTEMS | 1mg | EUR 374.5 |
Description: Human Glypican 3 (359-559) Protein, His Tag (GP3-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 359 - His 559 (Accession # P51654-1). |
Recombinant Human Glypican-1/GPC1 protein (His tag) |
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PDMH100051-100ug | Elabscience Biotech | 100ug | EUR 299 |
Description: Human |
Recombinant Human Glypican-1/GPC1 protein (His tag) |
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PDMH100051-20ug | Elabscience Biotech | 20ug | EUR 189 |
Description: Human |
Glypican 4 (GPC4) Protein |
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20-abx262759 | Abbexa |
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Glypican 4 (GPC4) Protein |
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abx262759-10mg | Abbexa | 10 mg | EUR 325 |
Glypican 4 (GPC4) Protein |
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abx262759-25mg | Abbexa | 25 mg | EUR 6575 |
Glypican 4 (GPC4) Protein |
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abx262759-5mg | Abbexa | 5 mg | EUR 225 |
Biotinylated Recombinant Human Glypican-3/GPC3 Protein |
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RP02371 | Abclonal | 1000μg | EUR 1423.5 |
GPC6 (untagged)-Human glypican 6 (GPC6) |
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SC116571 | Origene Technologies GmbH | 10 µg | Ask for price |
Human Glypican 3 Protein (Gln 25-His 559) [Fc] (FITC-Labeled) |
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VAng-1698Lsx-200g | Creative Biolabs | 200 µg | EUR 4184.4 |
Description: FITC-Labeled Human Glypican 3 (GPC3), Fc Tag, is expressed in human 293 cells. (Uniprot ID: P51654) |
Human Glypican 3 Protein (Gln 25-His 559) [Fc] (FITC-Labeled) |
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VAng-1698Lsx-25g | Creative Biolabs | 25 µg | EUR 1017.6 |
Description: FITC-Labeled Human Glypican 3 (GPC3), Fc Tag, is expressed in human 293 cells. (Uniprot ID: P51654) |
Human Glypican 3 Protein (Gln 25-His 559) [His] (FITC-Labeled) |
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VAng-1700Lsx-200g | Creative Biolabs | 200 µg | EUR 4184.4 |
Description: FITC-Labeled Human Glypican 3 (GPC3), His tag, is expressed in human 293 cells. (Uniprot ID: P51654-1) |
Human Glypican 3 Protein (Gln 25-His 559) [His] (FITC-Labeled) |
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VAng-1700Lsx-25g | Creative Biolabs | 25 µg | EUR 1017.6 |
Description: FITC-Labeled Human Glypican 3 (GPC3), His tag, is expressed in human 293 cells. (Uniprot ID: P51654-1) |
Recombinant Human Glypican 1/GPC1 Protein, His Tag |
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E40KMH1170 | EnoGene | 20ug | EUR 495 |
Recombinant Human Glypican 1/GPC1 Protein, His Tag |
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E40KMH1171 | EnoGene | 20ug | EUR 495 |
Recombinant Human Glypican 5/GPC5 Protein, His Tag |
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E40KMH1172 | EnoGene | 20ug | EUR 495 |
Human Glypican 3 Protein (Gln 25-His 559) [Llama IgG2b Fc Tag] |
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VAng-1702Lsx-100g | Creative Biolabs | 100 µg | EUR 1215.6 |
Description: Human Glypican 3 (GPC3), Llama IgG2b Fc Tag, is expressed in human 293 cells. (Uniprot ID: P51654-1) |
Human Glypican 3 Protein (Gln 25-His 559) [Llama IgG2b Fc Tag] |
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VAng-1702Lsx-1mg | Creative Biolabs | 1 mg | EUR 7682.4 |
Description: Human Glypican 3 (GPC3), Llama IgG2b Fc Tag, is expressed in human 293 cells. (Uniprot ID: P51654-1) |
Human Glypican 2 / GPC2 Protein, Fc Tag (SPR verified) |
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GP2-H5255 | ACROBIOSYSTEMS | 100ug | EUR 2749.9 |
Description: Human Glypican 2, Fc Tag (GP2-H5255) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Ser 553 (Accession # Q8N158-1). |
Human Glypican 3 / GPC3 (S495A, S509A) Protein, His Tag |
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GP3-H52H8 | ACROBIOSYSTEMS | 100ug | EUR 2749.9 |
Description: Human Glypican 3 (S495A, S509A), His Tag (GP3-H52H8) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1 (S495A, S509A)). |
Human Glypican 3 / GPC3 Protein, Fc Tag, low endotoxin |
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GP3-H5258 | ACROBIOSYSTEMS | 1mg | EUR 321 |
Description: Human Glypican 3, Fc Tag, low endotoxin (GP3-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1). |
Human Glypican 3 / GPC3 Protein, His Tag, premium grade |
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GP3-H52H4 | ACROBIOSYSTEMS | 100ug | EUR 2140 |
Description: Human Glypican 3, His Tag (GP3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1). |
FITC-Labeled Human Glypican 3 / GPC3 Protein, Fc Tag |
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GP3-HF258 | ACROBIOSYSTEMS | 25ug | EUR 2471.7 |
Description: FITC-Labeled Human Glypican 3, Fc Tag (Cat. No. GP3-HF258) is expressed from human HEK293 cells. It contains AA Gln 25 - His 559 (Accession # P51654-1). It is the FITC labeled form of Human Glypican 3, Fc Tag (Cat. No. GP3-H5258). |
Human Glypican 4 / GPC4 Protein, His Tag (MALS verified) |
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GP4-H52H3 | ACROBIOSYSTEMS | 100ug | EUR 2814.1 |
Description: Human Glypican 4, His Tag (GP4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Ser 529 (Accession # O75487-1). |
Rat Glypican 4 (GPC4) Protein |
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20-abx066895 | Abbexa |
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Rat Glypican 5 (GPC5) Protein |
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20-abx650459 | Abbexa |
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Rat Glypican 3 (GPC3) Protein |
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20-abx653617 | Abbexa |
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Rat Glypican 4 (GPC4) Protein |
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abx066895-100g | Abbexa | 100 µg | EUR 637.5 |
Rat Glypican 4 (GPC4) Protein |
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abx066895-10g | Abbexa | 10 µg | EUR 237.5 |
Rat Glypican 4 (GPC4) Protein |
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abx066895-50g | Abbexa | 50 µg | EUR 437.5 |
Rat Glypican 3 (GPC3) Protein |
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abx653617-50g | Abbexa | 50 µg | EUR 2112.5 |
Rat Glypican 5 (GPC5) Protein |
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abx650459-100g | Abbexa | 100 µg | EUR 450 |
Rat Glypican 5 (GPC5) Protein |
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abx650459-10g | Abbexa | 10 µg | EUR 187.5 |
Rat Glypican 5 (GPC5) Protein |
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abx650459-50g | Abbexa | 50 µg | EUR 325 |
Immunization with a RecombinantProtein of Trichinella britovi 14-3-Three Triggers an Immune Response nevertheless No Security in Mice
14-3-Three proteins are present in all eukaryotic organisms and are ubiquitously expressed in a broad fluctuate of tissues and cellular compartments. They’re regulatory adapter proteins that play key roles in a variety of signaling pathways, and have been proposed as applicable targets for the administration and detection of certain parasites.
- Trichinella britovi is a widely-distributed parasitic nematode, transmitted by the use of ingestion of meat merchandise containing invasive larvae. The present analysis describes the cloning and expression of Tb14-3-3, and investigates the immunological and defending potential of the recombinant protein. Immunization of mice with rTb14-3-Three triggered an IgG response, and essential variations, throughout the profiles of secreted cytokines observed in vitro, between experimental groups.
- Nonetheless, neither explicit antibodies, nor elevated secretion of IFNγ, IL-4, and IL-10 cytokines, conferred increased security in the direction of an an infection. No low cost in larval burden was observed all through restoration at 48 dpi. Furthermore, rTb14-3-Three was not acknowledged by sera from the contaminated administration mice, other than one, suggesting some mismatch between native and recombinant Tb14-3-Three antigenic web sites. Attributable to this reality, sooner than 14-3-Three could possibly be considered a potential software program for Trichinella detection and vaccination, further evaluation regarding its aim proteins, and exact explicit carry out, is required.
Inhibition of E. coli host RNA Polymerase Permits Setting pleasant Extracellular RecombinantProtein Manufacturing by Enhancing Outer Membrane Leakiness
Recombinant proteins in Escherichia coli are usually expressed contained within the cell. With the rising curiosity in regular cultivation, secretion of product to the medium is simply not solely a revenue nevertheless a necessity in future bioprocessing. On this analysis, we current that induced decoupling of progress and heterologous gene expression throughout the E. coli X-press stress, a stress derived from BL21(DE3), facilitates extracellular recombinant protein manufacturing. We investigated the influence of the strategy parameters temperature and explicit glucose consumption cost (qS ) on progress, productiveness, lysis and leakiness, to go looking out the parameter space allowing extracellular protein manufacturing.
- Two model proteins had been used, Protein A and a VHH single-domain antibody, and effectivity was compared with the industrial regular stress BL21(DE3). We current that inducible progress repression throughout the X-press stress vastly mitigates the influence of metabolic burden beneath completely completely different course of conditions.
- Furthermore, temperature and qS had been used to control productiveness and leakiness. Inside the X-press stress, extracellular Protein A and VHH titer reached as a lot as 349 mg/g and 19.6 mg/g, respectively, comprising as a lot as 90% of the total soluble product, whereas preserving cell lysis at a minimal. Our findings reveal that the X-press stress constitutes a helpful host for extracellular manufacturing of recombinant proteinwith E. coli. This textual content is protected by copyright. All rights reserved.
SV40 large T antigen NLS acetate |
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TP1830L-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: SV40 large T antigen NLS acetate |
SV40 large T antigen NLS acetate |
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TP1830L-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: SV40 large T antigen NLS acetate |
Recombinant Large T Antigen (LT) |
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RPU56223-100ug | Biomatik Corporation | 100ug | EUR 649 |
Recombinant Large T Antigen (LT) |
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RPU56223-1mg | Biomatik Corporation | 1mg | EUR 2558.4 |
Recombinant Large T Antigen (LT) |
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RPU56223-50ug | Biomatik Corporation | 50ug | EUR 393.6 |
Recombinant Large T Antigen (LT) |
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RPB142Ge01 | Cloud-Clone | 10ug | EUR 164 |
Recombinant Large T Antigen (LT) |
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RPB142Ge02 | Cloud-Clone | 10ug | EUR 168 |
Recombinant Large T Antigen (LT) |
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RPB142Ge03 | Cloud-Clone | 10ug | EUR 165 |
Recombinant HDV Large delta antigen |
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CSB-MP313702HFN(A4) | Cusabio | 7658 mg | Ask for price |
Sv40 Large T-antigen (CMV, Zeo) Lentivirus |
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LVP016-Zeo | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 245 |
Description: Lentivirus expresses SV40 large T-antigen under suCMV promoter, containing Zeocin selection. |
Sv40 Large T-antigen (EF1a, Zeo) Lentivirus |
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LVP557-Zeo | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 245 |
Description: Lentivirus expresses SV40 large T-antigen under EF1a promoter, containing Zeocin selection. |
Sv40 Large T-antigen (CMV, Hygro) Lentivirus |
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LVP016-Hygro | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 245 |
Description: Lentivirus expresses SV40 large T-antigen under suCMV promoter, containing Hygromycin selection. |
Sv40 Large T-antigen (EF1a, Hygro) Lentivirus |
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LVP557-Hygro | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 245 |
Description: Lentivirus expresses SV40 large T-antigen under EF1a promoter, containing Hygromycin selection. |
Simian Virus 40 large T (SV40 large T) ELISA Kit |
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abx055954-100g | Abbexa | 100 µg | Ask for price |
Simian Virus 40 large T (SV40 large T) ELISA Kit |
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abx055954-200g | Abbexa | 200 µg | Ask for price |
Simian Virus 40 large T (SV40 large T) ELISA Kit |
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abx055954-50g | Abbexa | 50 µg | EUR 493.75 |
SV40 Large T-antigen (CMV, no selection) lentivirus |
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LVP016 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 245 |
Description: lentiviral particles express SV40 large T-antigen under suCMV promoter, do not contain any selection marker. |
Echovirus Antigen, Recombinant |
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REC31776-100 | The Native Antigen Company | 0.1 | EUR 276.84 |
Description: Proprietary recombinant antigen expressed in E. coli and purified by chromatography. Individually pooled antigens shown to react with QC serum panel (multiple negative, borderline and positive sera) within defined reactivity range in Coxsackie-/Echovirus |
Echovirus Antigen, Recombinant |
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REC31776-500 | The Native Antigen Company | 0.5 | EUR 1043.56 |
Description: Proprietary recombinant antigen expressed in E. coli and purified by chromatography. Individually pooled antigens shown to react with QC serum panel (multiple negative, borderline and positive sera) within defined reactivity range in Coxsackie-/Echovirus |
SV40 large T-antigen (Bsd), CMV lentiviral particles |
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LVP016-Bsd | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing Blasticidin marker. |
SV40 large T-antigen (Neo), CMV lentiviral particles |
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LVP016-Neo | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing Neomycin marker. |
SV40 Large T-antigen (EF1a, no selection) lentivirus |
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LVP557 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 245 |
Description: lentiviral particles express SV40 large T-antigen under EF1a promoter, do not contain any selection marker. |
SV40 large T-antigen (Bsd), EF1a lentiviral particles |
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LVP557-Bsd | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing Blasticidin marker. |
SV40 large T-antigen (Neo), EF1a lentiviral particles |
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LVP557-Neo | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing Neomycin marker. |
SV40 large T-antigen (Puro), CMV lentiviral particles |
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LVP016-Puro | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing puromycin marker. |
Enterovirus Antigen, Recombinant |
|||
REC31772-100 | The Native Antigen Company | 0.1 | EUR 276.84 |
Description: Proprietary recombinant antigen expressed in E. coli and purified by chromatography. Individually pooled antigens shown to react with QC serum panel (multiple negative, borderline and positive sera) within defined reactivity range in Coxsackie-/Echovirus IgA, IgG and IgM ELISA. |
Enterovirus Antigen, Recombinant |
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REC31772-500 | The Native Antigen Company | 0.5 | EUR 1043.56 |
Description: Proprietary recombinant antigen expressed in E. coli and purified by chromatography. Individually pooled antigens shown to react with QC serum panel (multiple negative, borderline and positive sera) within defined reactivity range in Coxsackie-/Echovirus IgA, IgG and IgM ELISA. |
SV40 large T-antigen (Puro), EF1a lentiviral particles |
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LVP557-Puro | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing puromycin marker. |
Sv40 Large T-antigen (CMV, Zeo) Lentivirus in PBS |
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LVP016-Zeo-PBS | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
Description: concentrated Lentivirus expresses SV40 large T-antigen under suCMV promoter, containing Zeocin selection. |
Sv40 Large T-antigen (EF1a, Zeo) Lentivirus in PBS |
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LVP557-Zeo-PBS | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
Description: concentrated Lentivirus expresses SV40 large T-antigen under EF1a promoter, containing Zeocin selection. |
Sv40 Large T-antigen (CMV, Hygro) Lentivirus in PBS |
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LVP016-Hygro-PBS | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
Description: concentrated Lentivirus expresses SV40 large T-antigen under suCMV promoter, containing Hygromycin selection. |
Sv40 Large T-antigen (EF1a, Hygro) Lentivirus in PBS |
|||
LVP557-Hygro-PBS | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
Description: concentrated Lentivirus expresses SV40 large T-antigen under EF1a promoter, containing Hygromycin selection. |
Recombinant Hepatitis delta virus genotype I Large delta antigen |
|||
CSB-YP313702HFN | Cusabio | 6144 mg | Ask for price |
Recombinant Hepatitis delta virus genotype I Large delta antigen |
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AP77311 | SAB | each | Ask for price |
Recombinant Hepatitis delta virus genotype I Large delta antigen |
|||
AP78034 | SAB | each | Ask for price |
Recombinant Hepatitis delta virus genotype I Large delta antigen |
|||
CSB-BP313702HFN | Cusabio | 140 mg | Ask for price |
Recombinant Hepatitis delta virus genotype I Large delta antigen |
|||
CSB-BP313702HFN(A4) | Cusabio | 7409 mg | Ask for price |
Recombinant Hepatitis delta virus genotype I Large delta antigen |
|||
RPC27233-100ug | Biomatik Corporation | 100ug | EUR 1544.5 |
Recombinant Hepatitis delta virus genotype I Large delta antigen |
|||
RPC27233-1mg | Biomatik Corporation | 1mg | EUR 3933.6 |
Recombinant Hepatitis delta virus genotype I Large delta antigen |
|||
RPC27233-20ug | Biomatik Corporation | 20ug | EUR 580.8 |
Recombinant Hepatitis delta virus genotype I Large delta antigen |
|||
RPC20668-100ug | Biomatik Corporation | 100ug | EUR 1463 |
Recombinant Hepatitis delta virus genotype I Large delta antigen |
|||
RPC20668-20ug | Biomatik Corporation | 20ug | EUR 580.8 |
Ross River Virus Recombinant Antigen |
|||
REC31969-100 | The Native Antigen Company | 0.1 | EUR 757.8 |
Description: Recombinant Ross River virus antigen expressed in HEK293 cells as virus-like particles and purified by sucrose density gradients and precipitation. Virus-like particles were then solubilized in PBS containing 0.2% SDS to prevent aggregation of particles. The preparation is not particulate. Antigens in the preparation include Envelope proteins 1 and 2 and Nucleoprotein. |
Ross River Virus Recombinant Antigen |
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REC31969-500 | The Native Antigen Company | 0.5 | EUR 2844.92 |
Description: Recombinant Ross River virus antigen expressed in HEK293 cells as virus-like particles and purified by sucrose density gradients and precipitation. Virus-like particles were then solubilized in PBS containing 0.2% SDS to prevent aggregation of particles. The preparation is not particulate. Antigens in the preparation include Envelope proteins 1 and 2 and Nucleoprotein. |
SV40 large T-antigen (GFP-Bsd), CMV lentiviral particles |
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LVP557-GB | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing GFP-Blasticidin fusion dual marker. |
SV40 large T-antigen (GFP-Bsd), CMV lentiviral particles |
|||
LVP016-GB | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing GFP-Blasticidin fusion dual marker. |
SV40 large T-antigen (RFP-Bsd), CMV lentiviral particles |
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LVP016-RB | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing RFP-Blasticidin fusion dual marker. |
SV40 large T-antigen (RFP-Bsd), EF1a lentiviral particles |
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LVP557-RB | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing RFP-Blasticidin fusion dual marker. |
SV40 large T-antigen (GFP-Puro), CMV lentiviral particles |
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LVP016-GP | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing GFP-puromycin fusion dual marker. |
SV40 large T-antigen (RFP-Puro), CMV lentiviral particles |
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LVP016-RP | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing RFP-puromycin fusion dual marker. |
SV40 large T-antigen (GFP-Puro), EF1a lentiviral particles |
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LVP557-GP | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing GFP-puromycin fusion dual marker. |
SV40 large T-antigen (RFP-Puro), EF1a lentiviral particles |
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LVP557-RP | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing RFP-puromycin fusion dual marker. |
SV40 Large T-antigen (CMV, no selection) lentivirus in PBS |
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LVP016-PBS | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
Description: Concentrated lentivirus express SV40 large T-antigen under suCMV promoter, do not contain any selection marker, provided in PBS solution |
SV40 large T-antigen (Bsd) in PBS, CMV lentiviral particles |
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LVP016-Bsd-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing Blasticidin marker, provided in PBS solution. |
SV40 large T-antigen (Neo) in PBS, CMV lentiviral particles |
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LVP016-Neo-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing Neomycin marker, provided in PBS solution. |
SV40 Large T-antigen (EF1a, no selection) lentivirus in PBS |
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LVP557-PBS | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
Description: Concentrated lentivirus express SV40 large T-antigen under EF1a promoter, do not contain any selection marker, provided in PBS solution |
SV40 large T-antigen (Bsd) in PBS, EF1a lentiviral particles |
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LVP557-Bsd-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing Blasticidin marker, provided in PBS solution. |
SV40 large T-antigen (Neo) in PBS, EF1a lentiviral particles |
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LVP557-Neo-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing Neomycin marker, provided in PBS solution. |
SV40 large T-antigen (Puro) in PBS, CMV lentiviral particles |
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LVP016-Puro-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing puromycin marker, provided in PBS solution. |
SV40 large T-antigen (Puro) in PBS, EF1a lentiviral particles |
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LVP557-Puro-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing puromycin marker, provided in PBS solution. |
Mouse Anti-SV40 Large T Antigen Monoclonal antibody, clone I2 |
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DMAB-CS23064 | Creative Diagnostics | 200 μg | EUR 720 |
Description: Mouse |
SV40 T Antigen antibody |
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10R-2451 | Fitzgerald | 250 uL | EUR 550.8 |
Description: Mouse monoclonal SV40 Large T Antigen antibody |
SV40 T Antigen antibody |
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10R-S122a | Fitzgerald | 100 ug | EUR 950.4 |
Description: Mouse monoclonal SV40 T Antigen antibody |
SV40 T Antigen antibody |
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10R-S128a | Fitzgerald | 200 ug | EUR 734 |
Description: Mouse monoclonal SV40 T Antigen antibody |
SV40 large T-antigen (GFP-Bsd) in PBS, CMV lentiviral particles |
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LVP557-GB-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing GFP-Blasticidin fusion dual marker, provided in PBS solution. |
SV40 large T-antigen (GFP-Bsd) in PBS, CMV lentiviral particles |
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LVP016-GB-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing GFP-Blasticidin fusion dual marker, provided in PBS solution. |
SV40 large T-antigen (RFP-Bsd) in PBS, CMV lentiviral particles |
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LVP016-RB-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing RFP-Blasticidin fusion dual marker, provided in PBS solution. |
SV40 large T-antigen (RFP-Bsd) in PBS, EF1a lentiviral particles |
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LVP557-RB-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing RFP-Blasticidin fusion dual marker, provided in PBS solution. |
SV40 large T-antigen (GFP-Puro) in PBS, CMV lentiviral particles |
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LVP016-GP-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing GFP-puromycin fusion dual marker, provided in PBS solution. |
SV40 large T-antigen (RFP-Puro) in PBS, CMV lentiviral particles |
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LVP016-RP-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under suCMV promoter, containing RFP-puromycin fusion dual marker, provided in PBS solution. |
SV40 large T-antigen (GFP-Puro) in PBS, EF1a lentiviral particles |
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LVP557-GP-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing GFP-puromycin fusion dual marker, provided in PBS solution. |
SV40 large T-antigen (RFP-Puro) in PBS, EF1a lentiviral particles |
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LVP557-RP-PBS | GenTarget | 5x107 IFU/ml x 200ul | EUR 852 |
Description: Pre-made lentiviral particles expressing SV40 large T-antigen under EF1a promoter, containing RFP-puromycin fusion dual marker, provided in PBS solution. |
Large T Antigen (LT) Protein |
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20-abx654150 | Abbexa |
|
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Large T Antigen (LT) Protein |
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abx654150-10g | Abbexa | 10 µg | EUR 537.5 |
Large T Antigen (LT) Protein |
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abx654150-2g | Abbexa | 2 µg | EUR 375 |
Large T Antigen (LT) Antibody |
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20-abx177313 | Abbexa |
|
|
Large T Antigen (LT) Antibody |
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abx177313-96tests | Abbexa | 96 tests | EUR 287.5 |
SV40 T Antigen Rabbit mAb |
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E2R27328 | EnoGene | 100ul | EUR 255 |
Description: Available in various conjugation types. |
SV40 T Antigen Rabbit mAb |
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57396 | SAB | 100ul | EUR 399 |
SV40 small T antigen, Antibody |
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GWB-AF327B | GenWay Biotech | 0.1 mg | Ask for price |
Large T Antigen (LT) Polyclonal Antibody |
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CAU31357-100ul | Biomatik Corporation | 100ul | EUR 267.8 |
Large T Antigen (LT) Polyclonal Antibody |
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CAU31357-200ul | Biomatik Corporation | 200ul | EUR 334.3 |
SV40 T antigen (ab-1), Antibody |
|||
GWB-6116B9 | GenWay Biotech | 0.2 mg | Ask for price |
TSPyV Large T antigen Expression Lentivirus |
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LVP1385 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
Description: Lentivirus express Large T antigen of TSPyV (Trichodysplasia spinulosa-associated polyomavirus), containing RFP-Blasticidin dual selection. |
MCPyV Large T antigen Expression Lentivirus |
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LVP1386 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
Description: Lentivirus express Large T antigen of MCPyV (Merkel cell polyomavirus), containing RFP-Blasticidin dual selection. |
SV40 T Antigen Cell Immortalization Kit |
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CILV01 | ALSTEM | 1 Kit | EUR 1094.8 |
Polyclonal Antibody to Large T Antigen (LT) |
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PAB142Ge01 | Cloud-Clone | 100ul | EUR 279 |
SV40 T Antigen Rabbit Monoclonal Antibody |
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E10G22593 | EnoGene | 100 μl | EUR 275 |
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
Large T antigen - rhesus polyomavirus 560-568 |
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A1078-1 | ApexBio | 1 mg | EUR 40 |
Description: Large T antigen |
Large T antigen - rhesus polyomavirus 560-568 |
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A1078-10 | ApexBio | 10 mg | EUR 200 |
Description: Large T antigen |